Values CatLog read from this paper
Metal-cation regulation of enzyme dynamics is a key factor influencing the activity of S-adenosyl-L-homocysteine hydrolase from Pseudomonas aeruginosa
PMID 30054521doi:10.1038/s41598-018-29535-yOpen in Browse
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CatLog read 7 records (21 values) from this paper: S-adenosyl-L-homocysteine hydrolase (PaSAHase) from Pseudomonas aeruginosa.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| S-adenosyl-L-homocysteine hydrolase (PaSAHase) | Pseudomonas aeruginosa | S-adenosyl-L-homocysteine100 mM Tris·HCl pH 7.5; no alkali metal cation | 0.0529 | 0.1011 | 0.523 | pH 7.5, 20 °C | Open record: S-adenosyl-L-homocysteine hydrolase (PaSAHase), S-adenosyl-L-homocysteine |
| S-adenosyl-L-homocysteine hydrolase (PaSAHase) | Pseudomonas aeruginosa | S-adenosyl-L-homocysteine100 mM LiCl; 25 mM HEPES·LiOH pH 7.5 | 0.0466 | 0.0638 | 0.731 | pH 7.5, 20 °C | Open record: S-adenosyl-L-homocysteine hydrolase (PaSAHase), S-adenosyl-L-homocysteine |
| S-adenosyl-L-homocysteine hydrolase (PaSAHase) | Pseudomonas aeruginosa | S-adenosyl-L-homocysteine100 mM NaCl; 25 mM HEPES·NaOH pH 7.5 | 0.2373 | 0.028 | 8.467 | pH 7.5, 20 °C | Open record: S-adenosyl-L-homocysteine hydrolase (PaSAHase), S-adenosyl-L-homocysteine |
| S-adenosyl-L-homocysteine hydrolase (PaSAHase) | Pseudomonas aeruginosa | S-adenosyl-L-homocysteine100 mM KCl; 25 mM HEPES·KOH pH 7.5 | 0.575 | 0.0079 | 72.50 | pH 7.5, 20 °C | Open record: S-adenosyl-L-homocysteine hydrolase (PaSAHase), S-adenosyl-L-homocysteine |
| S-adenosyl-L-homocysteine hydrolase (PaSAHase) | Pseudomonas aeruginosa | S-adenosyl-L-homocysteine100 mM RbCl; 25 mM HEPES·RbOH pH 7.5 | 0.0175 | 0.0049 | 3.568 | pH 7.5, 20 °C | Open record: S-adenosyl-L-homocysteine hydrolase (PaSAHase), S-adenosyl-L-homocysteine |
| S-adenosyl-L-homocysteine hydrolase (PaSAHase) | Pseudomonas aeruginosa | S-adenosyl-L-homocysteine100 mM CsCl; 25 mM HEPES·CsOH pH 7.5 | 0.2131 | 0.032 | 6.668 | pH 7.5, 20 °C | Open record: S-adenosyl-L-homocysteine hydrolase (PaSAHase), S-adenosyl-L-homocysteine |
| S-adenosyl-L-homocysteine hydrolase (PaSAHase)variant Q65A | Pseudomonas aeruginosa | S-adenosyl-L-homocysteine | 0.14 | 0.0262 | 5.322 | pH 7.5, 20 °C | Open record: S-adenosyl-L-homocysteine hydrolase (PaSAHase) variant Q65A, S-adenosyl-L-homocysteine |