Values CatLog read from this paper
Pyridine Nucleotide Coenzyme Specificity of p-Hydroxybenzoate Hydroxylase and Related Flavoprotein Monooxygenases
PMID 30631308doi:10.3389/fmicb.2018.03050Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (28 values) from this paper: p-hydroxybenzoate hydroxylase PHBH_Ro1CP, p-hydroxybenzoate hydroxylase PHBH_Cn1, p-hydroxybenzoate hydroxylase PHBH_Cn2 from Cupriavidus necator, Rhodococcus opacus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 14 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| p-hydroxybenzoate hydroxylase PHBH_Cn1 | Cupriavidus necator | 4-hydroxybenzoateconstant NADH 175 μM; UV/VIS: NAD(P)H consumption at 340 nm | 7.6 | 0.0202 | 380 calc. | pH 7.5, 30 °C | Open record: p-hydroxybenzoate hydroxylase PHBH_Cn1, 4-hydroxybenzoate |
| p-hydroxybenzoate hydroxylase PHBH_Cn1 | Cupriavidus necator | 4-hydroxybenzoateconstant NADH 175 μM; cosubstrate NADH | 6.8 | 0.0193 | 350 calc. | pH 7.5, 30 °C | Open record: p-hydroxybenzoate hydroxylase PHBH_Cn1, 4-hydroxybenzoate |
| p-hydroxybenzoate hydroxylase PHBH_Cn1 | Cupriavidus necator | 4-hydroxybenzoateconstant NADPH 175 μM; UV/VIS: NAD(P)H consumption at 340 nm | 6.5 | 0.0194 | 340 calc. | pH 7.5, 30 °C | Open record: p-hydroxybenzoate hydroxylase PHBH_Cn1, 4-hydroxybenzoate |
| p-hydroxybenzoate hydroxylase PHBH_Cn1 | Cupriavidus necator | 4-hydroxybenzoateconstant NADPH 175 μM; cosubstrate NADPH | 4.6 | 0.0204 | 230 calc. | pH 7.5, 30 °C | Open record: p-hydroxybenzoate hydroxylase PHBH_Cn1, 4-hydroxybenzoate |
| p-hydroxybenzoate hydroxylase PHBH_Cn1 | Cupriavidus necator | NADH | 9.4 | 0.0498 | 190 calc. | pH 7.5, 30 °C | Open record: p-hydroxybenzoate hydroxylase PHBH_Cn1, NADH |
| p-hydroxybenzoate hydroxylase PHBH_Cn2 | Cupriavidus necator | 4-hydroxybenzoateUV/VIS: NAD(P)H consumption at 340 nm | 18 | 0.0264 | 680 calc. | pH 7.5, 30 °C | Open record: p-hydroxybenzoate hydroxylase PHBH_Cn2, 4-hydroxybenzoate |
| p-hydroxybenzoate hydroxylase PHBH_Cn2 | Cupriavidus necator | 4-hydroxybenzoateHPLC: 3,4-dihydroxybenzoate product formation | 30.4 | 0.0306 | 993 calc. | pH 7.5, 30 °C | Open record: p-hydroxybenzoate hydroxylase PHBH_Cn2, 4-hydroxybenzoate |
| p-hydroxybenzoate hydroxylase PHBH_Cn2 | Cupriavidus necator | NADPH | 37 | 0.146 | 250 calc. | pH 7.5, 30 °C | Open record: p-hydroxybenzoate hydroxylase PHBH_Cn2, NADPH |
| p-hydroxybenzoate hydroxylase PHBH_Ro1CP | Rhodococcus opacus | 4-hydroxybenzoateconstant NADH 175 μM; UV/VIS: NAD(P)H consumption at 340 nm | 12.9 | 0.0304 | 424 calc. | pH 7.5, 30 °C | Open record: p-hydroxybenzoate hydroxylase PHBH_Ro1CP, 4-hydroxybenzoate |
| p-hydroxybenzoate hydroxylase PHBH_Ro1CP | Rhodococcus opacus | 4-hydroxybenzoateconstant NADH 175 μM; cosubstrate NADH | 16.8 | 0.0499 | 337 calc. | pH 7.5, 30 °C | Open record: p-hydroxybenzoate hydroxylase PHBH_Ro1CP, 4-hydroxybenzoate |
| p-hydroxybenzoate hydroxylase PHBH_Ro1CP | Rhodococcus opacus | 4-hydroxybenzoateconstant NADPH 175 μM; UV/VIS: NAD(P)H consumption at 340 nm | 8.7 | 0.035 | 250 calc. | pH 7.5, 30 °C | Open record: p-hydroxybenzoate hydroxylase PHBH_Ro1CP, 4-hydroxybenzoate |
| p-hydroxybenzoate hydroxylase PHBH_Ro1CP | Rhodococcus opacus | 4-hydroxybenzoateconstant NADPH 175 μM; cosubstrate NADPH | 9.6 | 0.042 | 230 calc. | pH 7.5, 30 °C | Open record: p-hydroxybenzoate hydroxylase PHBH_Ro1CP, 4-hydroxybenzoate |
| p-hydroxybenzoate hydroxylase PHBH_Ro1CP | Rhodococcus opacus | NADH | 15.3 | 0.0397 | 385 calc. | pH 7.5, 30 °C | Open record: p-hydroxybenzoate hydroxylase PHBH_Ro1CP, NADH |
| p-hydroxybenzoate hydroxylase PHBH_Ro1CP | Rhodococcus opacus | NADPH | 15 | 0.153 | 98 calc. | pH 7.5, 30 °C | Open record: p-hydroxybenzoate hydroxylase PHBH_Ro1CP, NADPH |