EC 1.14.13.2: 4-hydroxybenzoate 3-monooxygenase
OxidoreductasesOpen in Browse
CatLog read 32 records for EC 1.14.13.2 from 4 papers, for 4 organisms. Names in these papers: 4-hydroxybenzoate hydroxylase (PobA), p-hydroxybenzoate hydroxylase PHBH_Ro1CP, p-hydroxybenzoate hydroxylase PHBH_Cn1, PobA (para-hydroxybenzoate-3-hydroxylase) and 2 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 34 records for EC 1.14.13.2 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.14.13.2 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Pyridine Nucleotide Coenzyme Specificity of p-Hydroxybenzoate Hydroxylase and Related Flavoprotein Monooxygenases | Cupriavidus necator, Rhodococcus opacus | 14 |
| A Growth-Based, High-Throughput Selection Platform Enables Remodeling of 4-Hydroxybenzoate Hydroxylase Active Site | Pseudomonas aeruginosa | 10 |
| Debottlenecking 4-hydroxybenzoate hydroxylation in Pseudomonas putida KT2440 improves muconate productivity from p-coumarate | Pseudomonas putida | 5 |
| Understanding the Molecular Mechanism Underlying the High Catalytic Activity of p-Hydroxybenzoate Hydroxylase Mutants for Producing Gallic Acid | Pseudomonas aeruginosa | 3 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| 4-hydroxybenzoate hydroxylase (PobA) | Pseudomonas aeruginosa | 4-hydroxybenzoic acid | 7.6 | 0.048 | 160 | PMID 34295569 | Open record: 4-hydroxybenzoate hydroxylase (PobA), 4-hydroxybenzoic acid | |
| 4-hydroxybenzoate hydroxylase (PobA) | Pseudomonas aeruginosa | NADPH | 7.8 | 0.25 | 31 | PMID 34295569 | Open record: 4-hydroxybenzoate hydroxylase (PobA), NADPH | |
| 4-hydroxybenzoate hydroxylase (PobA)variant L199R/T294C/Y385M | Pseudomonas aeruginosa | 3,4-dihydroxybenzoic acid | 3 | 0.048 | 62 | PMID 34295569 | Open record: 4-hydroxybenzoate hydroxylase (PobA) variant L199R/T294C/Y385M, 3,4-dihydroxybenzoic acid | |
| 4-hydroxybenzoate hydroxylase (PobA)variant L199R/T294C/Y385M | Pseudomonas aeruginosa | 4-hydroxybenzoic acid | 1.9 | 0.024 | 79 | PMID 34295569 | Open record: 4-hydroxybenzoate hydroxylase (PobA) variant L199R/T294C/Y385M, 4-hydroxybenzoic acid | |
| 4-hydroxybenzoate hydroxylase (PobA)variant L199R/T294C/Y385M | Pseudomonas aeruginosa | NADPHin vitro assay (cosubstrate 4-HBA); cosubstrate 4-HBA | 2.1 | 0.047 | 44 | PMID 34295569 | Open record: 4-hydroxybenzoate hydroxylase (PobA) variant L199R/T294C/Y385M, NADPH | |
| 4-hydroxybenzoate hydroxylase (PobA)variant L199R/T294C/Y385M | Pseudomonas aeruginosa | NADPHin vitro assay (cosubstrate 3,4-DHBA); cosubstrate 3,4-DHBA | 2.5 | 0.076 | 33 | PMID 34295569 | Open record: 4-hydroxybenzoate hydroxylase (PobA) variant L199R/T294C/Y385M, NADPH | |
| 4-hydroxybenzoate hydroxylase (PobA)variant V47I/L199N/T294A/Y385I | Pseudomonas aeruginosa | 3,4-dihydroxybenzoic acid | 4.1 | 0.12 | 33 | PMID 34295569 | Open record: 4-hydroxybenzoate hydroxylase (PobA) variant V47I/L199N/T294A/Y385I, 3,4-dihydroxybenzoic acid | |
| 4-hydroxybenzoate hydroxylase (PobA)variant V47I/L199N/T294A/Y385I | Pseudomonas aeruginosa | 4-hydroxybenzoic acid | 4 | 0.12 | 34 | PMID 34295569 | Open record: 4-hydroxybenzoate hydroxylase (PobA) variant V47I/L199N/T294A/Y385I, 4-hydroxybenzoic acid | |
| 4-hydroxybenzoate hydroxylase (PobA)variant V47I/L199N/T294A/Y385I | Pseudomonas aeruginosa | NADPHin vitro assay (cosubstrate 4-HBA); cosubstrate 4-HBA | 4.4 | 0.14 | 32 | PMID 34295569 | Open record: 4-hydroxybenzoate hydroxylase (PobA) variant V47I/L199N/T294A/Y385I, NADPH | |
| 4-hydroxybenzoate hydroxylase (PobA)variant V47I/L199N/T294A/Y385I | Pseudomonas aeruginosa | NADPHin vitro assay (cosubstrate 3,4-DHBA); cosubstrate 3,4-DHBA | 4.1 | 0.12 | 33 | PMID 34295569 | Open record: 4-hydroxybenzoate hydroxylase (PobA) variant V47I/L199N/T294A/Y385I, NADPH | |
| p-hydroxybenzoate hydroxylase (PHBH) | Pseudomonas aeruginosa | p-hydroxybenzoate | 8.9 | 0.039 | 230 | pH 7.5, 30 °C | PMID 31639299 | Open record: p-hydroxybenzoate hydroxylase (PHBH), p-hydroxybenzoate |
| p-hydroxybenzoate hydroxylase (PHBH)variant L199V/Y385F | Pseudomonas aeruginosa | 3,4-dihydroxybenzoate | 4.4 | 0.052 | 85 | pH 7.5, 30 °C | PMID 31639299 | Open record: p-hydroxybenzoate hydroxylase (PHBH) variant L199V/Y385F, 3,4-dihydroxybenzoate |
| p-hydroxybenzoate hydroxylase (PHBH)variant L199V/Y385F | Pseudomonas aeruginosa | p-hydroxybenzoate | 0.45 | 0.022 | 21 | pH 7.5, 30 °C | PMID 31639299 | Open record: p-hydroxybenzoate hydroxylase (PHBH) variant L199V/Y385F, p-hydroxybenzoate |
| p-hydroxybenzoate hydroxylase PHBH_Cn1 | Cupriavidus necator | 4-hydroxybenzoateconstant NADH 175 μM; UV/VIS: NAD(P)H consumption at 340 nm | 7.6 | 0.0202 | 380 calc. | pH 7.5, 30 °C | PMID 30631308 | Open record: p-hydroxybenzoate hydroxylase PHBH_Cn1, 4-hydroxybenzoate |
| p-hydroxybenzoate hydroxylase PHBH_Cn1 | Cupriavidus necator | 4-hydroxybenzoateconstant NADH 175 μM; cosubstrate NADH | 6.8 | 0.0193 | 350 calc. | pH 7.5, 30 °C | PMID 30631308 | Open record: p-hydroxybenzoate hydroxylase PHBH_Cn1, 4-hydroxybenzoate |
| p-hydroxybenzoate hydroxylase PHBH_Cn1 | Cupriavidus necator | 4-hydroxybenzoateconstant NADPH 175 μM; UV/VIS: NAD(P)H consumption at 340 nm | 6.5 | 0.0194 | 340 calc. | pH 7.5, 30 °C | PMID 30631308 | Open record: p-hydroxybenzoate hydroxylase PHBH_Cn1, 4-hydroxybenzoate |
| p-hydroxybenzoate hydroxylase PHBH_Cn1 | Cupriavidus necator | 4-hydroxybenzoateconstant NADPH 175 μM; cosubstrate NADPH | 4.6 | 0.0204 | 230 calc. | pH 7.5, 30 °C | PMID 30631308 | Open record: p-hydroxybenzoate hydroxylase PHBH_Cn1, 4-hydroxybenzoate |
| p-hydroxybenzoate hydroxylase PHBH_Cn1 | Cupriavidus necator | NADH | 9.4 | 0.0498 | 190 calc. | pH 7.5, 30 °C | PMID 30631308 | Open record: p-hydroxybenzoate hydroxylase PHBH_Cn1, NADH |
| p-hydroxybenzoate hydroxylase PHBH_Cn2 | Cupriavidus necator | 4-hydroxybenzoateUV/VIS: NAD(P)H consumption at 340 nm | 18 | 0.0264 | 680 calc. | pH 7.5, 30 °C | PMID 30631308 | Open record: p-hydroxybenzoate hydroxylase PHBH_Cn2, 4-hydroxybenzoate |
| p-hydroxybenzoate hydroxylase PHBH_Cn2 | Cupriavidus necator | 4-hydroxybenzoateHPLC: 3,4-dihydroxybenzoate product formation | 30.4 | 0.0306 | 993 calc. | pH 7.5, 30 °C | PMID 30631308 | Open record: p-hydroxybenzoate hydroxylase PHBH_Cn2, 4-hydroxybenzoate |
| p-hydroxybenzoate hydroxylase PHBH_Cn2 | Cupriavidus necator | NADPH | 37 | 0.146 | 250 calc. | pH 7.5, 30 °C | PMID 30631308 | Open record: p-hydroxybenzoate hydroxylase PHBH_Cn2, NADPH |
| p-hydroxybenzoate hydroxylase PHBH_Ro1CP | Rhodococcus opacus | 4-hydroxybenzoateconstant NADH 175 μM; UV/VIS: NAD(P)H consumption at 340 nm | 12.9 | 0.0304 | 424 calc. | pH 7.5, 30 °C | PMID 30631308 | Open record: p-hydroxybenzoate hydroxylase PHBH_Ro1CP, 4-hydroxybenzoate |
| p-hydroxybenzoate hydroxylase PHBH_Ro1CP | Rhodococcus opacus | 4-hydroxybenzoateconstant NADH 175 μM; cosubstrate NADH | 16.8 | 0.0499 | 337 calc. | pH 7.5, 30 °C | PMID 30631308 | Open record: p-hydroxybenzoate hydroxylase PHBH_Ro1CP, 4-hydroxybenzoate |
| p-hydroxybenzoate hydroxylase PHBH_Ro1CP | Rhodococcus opacus | 4-hydroxybenzoateconstant NADPH 175 μM; UV/VIS: NAD(P)H consumption at 340 nm | 8.7 | 0.035 | 250 calc. | pH 7.5, 30 °C | PMID 30631308 | Open record: p-hydroxybenzoate hydroxylase PHBH_Ro1CP, 4-hydroxybenzoate |
| p-hydroxybenzoate hydroxylase PHBH_Ro1CP | Rhodococcus opacus | 4-hydroxybenzoateconstant NADPH 175 μM; cosubstrate NADPH | 9.6 | 0.042 | 230 calc. | pH 7.5, 30 °C | PMID 30631308 | Open record: p-hydroxybenzoate hydroxylase PHBH_Ro1CP, 4-hydroxybenzoate |
| p-hydroxybenzoate hydroxylase PHBH_Ro1CP | Rhodococcus opacus | NADH | 15.3 | 0.0397 | 385 calc. | pH 7.5, 30 °C | PMID 30631308 | Open record: p-hydroxybenzoate hydroxylase PHBH_Ro1CP, NADH |
| p-hydroxybenzoate hydroxylase PHBH_Ro1CP | Rhodococcus opacus | NADPH | 15 | 0.153 | 98 calc. | pH 7.5, 30 °C | PMID 30631308 | Open record: p-hydroxybenzoate hydroxylase PHBH_Ro1CP, NADPH |
| PobA (para-hydroxybenzoate-3-hydroxylase) | Pseudomonas putida | 4-hydroxybenzoatespectrophotometric detection (NAD(P)H loss at 340 nm) | 54 | 0.018 | 3 × 10³ | pH 8, 25 °C | PMID 34982998 | Open record: PobA (para-hydroxybenzoate-3-hydroxylase), 4-hydroxybenzoate |
| PobA (para-hydroxybenzoate-3-hydroxylase) | Pseudomonas putida | 4-hydroxybenzoateoxygraph detection (O2 depletion, Clark-type electrode) | 40 | 0.021 | 1.9 × 10³ | pH 8, 25 °C | PMID 34982998 | Open record: PobA (para-hydroxybenzoate-3-hydroxylase), 4-hydroxybenzoate |
| PobA (para-hydroxybenzoate-3-hydroxylase) | Pseudomonas putida | dioxygen | 53 | 0.07 | 760 | pH 8, 25 °C | PMID 34982998 | Open record: PobA (para-hydroxybenzoate-3-hydroxylase), dioxygen |
| PobA (para-hydroxybenzoate-3-hydroxylase) | Pseudomonas putida | NADPHspectrophotometric detection (NAD(P)H loss at 340 nm) | 50 | 0.043 | 1.2 × 10³ | pH 8, 25 °C | PMID 34982998 | Open record: PobA (para-hydroxybenzoate-3-hydroxylase), NADPH |
| PobA (para-hydroxybenzoate-3-hydroxylase) | Pseudomonas putida | NADPHoxygraph detection (O2 depletion, Clark-type electrode) | 47 | 0.066 | 710 | pH 8, 25 °C | PMID 34982998 | Open record: PobA (para-hydroxybenzoate-3-hydroxylase), NADPH |