Values CatLog read from this paper
Debottlenecking 4-hydroxybenzoate hydroxylation in Pseudomonas putida KT2440 improves muconate productivity from p-coumarate
PMID 34982998doi:10.1016/j.ymben.2021.12.010Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 15 records (45 values) from this paper: PraI (para-hydroxybenzoate-3-hydroxylase), PobA (para-hydroxybenzoate-3-hydroxylase) from Paenibacillus sp. JJ-1b, Pseudomonas putida.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| PobA (para-hydroxybenzoate-3-hydroxylase) | Pseudomonas putida | 4-hydroxybenzoatespectrophotometric detection (NAD(P)H loss at 340 nm) | 54 | 0.018 | 3 × 10³ | pH 8, 25 °C | Open record: PobA (para-hydroxybenzoate-3-hydroxylase), 4-hydroxybenzoate |
| PobA (para-hydroxybenzoate-3-hydroxylase) | Pseudomonas putida | 4-hydroxybenzoateoxygraph detection (O2 depletion, Clark-type electrode) | 40 | 0.021 | 1.9 × 10³ | pH 8, 25 °C | Open record: PobA (para-hydroxybenzoate-3-hydroxylase), 4-hydroxybenzoate |
| PobA (para-hydroxybenzoate-3-hydroxylase) | Pseudomonas putida | dioxygen | 53 | 0.07 | 760 | pH 8, 25 °C | Open record: PobA (para-hydroxybenzoate-3-hydroxylase), dioxygen |
| PobA (para-hydroxybenzoate-3-hydroxylase) | Pseudomonas putida | NADPHspectrophotometric detection (NAD(P)H loss at 340 nm) | 50 | 0.043 | 1.2 × 10³ | pH 8, 25 °C | Open record: PobA (para-hydroxybenzoate-3-hydroxylase), NADPH |
| PobA (para-hydroxybenzoate-3-hydroxylase) | Pseudomonas putida | NADPHoxygraph detection (O2 depletion, Clark-type electrode) | 47 | 0.066 | 710 | pH 8, 25 °C | Open record: PobA (para-hydroxybenzoate-3-hydroxylase), NADPH |
| PraI (para-hydroxybenzoate-3-hydroxylase) | Paenibacillus sp. JJ-1b | 4-hydroxybenzoatespectrophotometric detection (NAD(P)H loss at 340 nm); NADPH | 27 | 0.016 | 1.7 × 10³ | pH 8, 25 °C | Open record: PraI (para-hydroxybenzoate-3-hydroxylase), 4-hydroxybenzoate |
| PraI (para-hydroxybenzoate-3-hydroxylase) | Paenibacillus sp. JJ-1b | 4-hydroxybenzoateoxygraph detection (O2 depletion, Clark-type electrode) | 23 | 0.019 | 1.9 × 10³ | pH 8, 25 °C | Open record: PraI (para-hydroxybenzoate-3-hydroxylase), 4-hydroxybenzoate |
| PraI (para-hydroxybenzoate-3-hydroxylase) | Paenibacillus sp. JJ-1b | 4-hydroxybenzoatespectrophotometric detection (NAD(P)H loss at 340 nm); NADH | 26 | 0.018 | 1.4 × 10³ | pH 8, 25 °C | Open record: PraI (para-hydroxybenzoate-3-hydroxylase), 4-hydroxybenzoate |
| PraI (para-hydroxybenzoate-3-hydroxylase) | Paenibacillus sp. JJ-1b | 4-hydroxybenzoateoxygraph detection (O2 depletion, Clark-type electrode) | 21 | 0.016 | 1.3 × 10³ | pH 8, 25 °C | Open record: PraI (para-hydroxybenzoate-3-hydroxylase), 4-hydroxybenzoate |
| PraI (para-hydroxybenzoate-3-hydroxylase) | Paenibacillus sp. JJ-1b | dioxygenreducing equivalent NADPH; NADPH | 25 | 0.068 | 370 | pH 8, 25 °C | Open record: PraI (para-hydroxybenzoate-3-hydroxylase), dioxygen |
| PraI (para-hydroxybenzoate-3-hydroxylase) | Paenibacillus sp. JJ-1b | dioxygenreducing equivalent NADH; NADH | 22 | 0.054 | 400 | pH 8, 25 °C | Open record: PraI (para-hydroxybenzoate-3-hydroxylase), dioxygen |
| PraI (para-hydroxybenzoate-3-hydroxylase) | Paenibacillus sp. JJ-1b | NADHspectrophotometric detection (NAD(P)H loss at 340 nm) | 25 | 0.049 | 510 | pH 8, 25 °C | Open record: PraI (para-hydroxybenzoate-3-hydroxylase), NADH |
| PraI (para-hydroxybenzoate-3-hydroxylase) | Paenibacillus sp. JJ-1b | NADHoxygraph detection (O2 depletion, Clark-type electrode) | 22 | 0.046 | 480 | pH 8, 25 °C | Open record: PraI (para-hydroxybenzoate-3-hydroxylase), NADH |
| PraI (para-hydroxybenzoate-3-hydroxylase) | Paenibacillus sp. JJ-1b | NADPHspectrophotometric detection (NAD(P)H loss at 340 nm) | 27 | 0.027 | 1 × 10³ | pH 8, 25 °C | Open record: PraI (para-hydroxybenzoate-3-hydroxylase), NADPH |
| PraI (para-hydroxybenzoate-3-hydroxylase) | Paenibacillus sp. JJ-1b | NADPHoxygraph detection (O2 depletion, Clark-type electrode) | 23 | 0.033 | 690 | pH 8, 25 °C | Open record: PraI (para-hydroxybenzoate-3-hydroxylase), NADPH |