Values CatLog read from this paper

Enhanced activity and substrate tolerance of 7α-hydroxysteroid dehydrogenase by directed evolution for 7-ketolithocholic acid production

PMID 30734123doi:10.1007/s00253-019-09668-4Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 4 records (12 values) from this paper: 7α-hydroxysteroid dehydrogenase (CA 7α-HSDH) from Clostridium absonum.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.159

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
7α-hydroxysteroid dehydrogenase (CA 7α-HSDH)	Clostridium absonum	chenodeoxycholic acid	2.34	0.0661	35.4	pH 8, 25 °C	Open record: 7α-hydroxysteroid dehydrogenase (CA 7α-HSDH), chenodeoxycholic acid
7α-hydroxysteroid dehydrogenase (CA 7α-HSDH)	Clostridium absonum	nicotinamide adenine dinucleotide phosphate (oxidized form)	3.66	0.3116	11.8	pH 8, 25 °C	Open record: 7α-hydroxysteroid dehydrogenase (CA 7α-HSDH), nicotinamide adenine dinucleotide phosphate (oxidized form)
7α-hydroxysteroid dehydrogenase (CA 7α-HSDH)variant Q255L/C260S	Clostridium absonum	chenodeoxycholic acid	21	0.0601	349.4	pH 8, 25 °C	Open record: 7α-hydroxysteroid dehydrogenase (CA 7α-HSDH) variant Q255L/C260S, chenodeoxycholic acid
7α-hydroxysteroid dehydrogenase (CA 7α-HSDH)variant Q255L/C260S	Clostridium absonum	nicotinamide adenine dinucleotide phosphate (oxidized form)	18.45	0.1094	168.6	pH 8, 25 °C	Open record: 7α-hydroxysteroid dehydrogenase (CA 7α-HSDH) variant Q255L/C260S, nicotinamide adenine dinucleotide phosphate (oxidized form)