Values CatLog read from this paper
Altering the Substrate Specificity of Acetyl-CoA Synthetase by Rational Mutagenesis of the Carboxylate Binding Pocket
PMID 31117358doi:10.1021/acssynbio.9b00008Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 83 records (232 values) from this paper: acetyl-CoA synthetase (atACS) from Arabidopsis thaliana.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 15 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| acetyl-CoA synthetase (atACS) | Arabidopsis thaliana | acetateTris-HCl extraction buffer preparation | 2.41 | 0.27 | 8.9 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS), acetate |
| acetyl-CoA synthetase (atACS) | Arabidopsis thaliana | acetateHEPES extraction buffer preparation | 5.7 | 0.12 | 48 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS), acetate |
| acetyl-CoA synthetase (atACS) | Arabidopsis thaliana | adenosine 5'-triphosphateTris-HCl extraction buffer preparation | 3.56 | 0.2 | 18 calc. | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS), adenosine 5'-triphosphate |
| acetyl-CoA synthetase (atACS) | Arabidopsis thaliana | adenosine 5'-triphosphateHEPES extraction buffer preparation | 16.04 | 0.05 | 320 calc. | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS), adenosine 5'-triphosphate |
| acetyl-CoA synthetase (atACS) | Arabidopsis thaliana | coenzyme ATris-HCl extraction buffer preparation | 3.47 | 0.15 | 23 calc. | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS), coenzyme A |
| acetyl-CoA synthetase (atACS) | Arabidopsis thaliana | coenzyme AHEPES extraction buffer preparation | 16.03 | 0.11 | 150 calc. | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS), coenzyme A |
| acetyl-CoA synthetase (atACS) | Arabidopsis thaliana | propionate | 0.66 | 4 | 0.16 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS), propionate |
| acetyl-CoA synthetase (atACS)variant I323A/V399A/W427G | Arabidopsis thaliana | 2-methylpropanoate | 3.2 | 110 | 0.03 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant I323A/V399A/W427G, 2-methylpropanoate |
| acetyl-CoA synthetase (atACS)variant I323A/V399A/W427G | Arabidopsis thaliana | 3-methylpentanoate | 1.9 | 1.5 | 1.22 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant I323A/V399A/W427G, 3-methylpentanoate |
| acetyl-CoA synthetase (atACS)variant I323A/V399A/W427G | Arabidopsis thaliana | 4-methylhexanoate | 1.5 | 1 | 1.6 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant I323A/V399A/W427G, 4-methylhexanoate |
| acetyl-CoA synthetase (atACS)variant I323A/V399A/W427G | Arabidopsis thaliana | 4-methylpentanoate | 6.7 | 4.3 | 1.57 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant I323A/V399A/W427G, 4-methylpentanoate |
| acetyl-CoA synthetase (atACS)variant I323A/V399A/W427G | Arabidopsis thaliana | adenosine 5'-triphosphate | 1.6 | 0.11 | 15 calc. | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant I323A/V399A/W427G, adenosine 5'-triphosphate |
| acetyl-CoA synthetase (atACS)variant I323A/V399A/W427G | Arabidopsis thaliana | coenzyme A | 1.45 | 0.04 | 36 calc. | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant I323A/V399A/W427G, coenzyme A |
| acetyl-CoA synthetase (atACS)variant I323A/V399A/W427G | Arabidopsis thaliana | heptanoate | 2.22 | 1.05 | 2.11 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant I323A/V399A/W427G, heptanoate |
| acetyl-CoA synthetase (atACS)variant I323A/V399A/W427G | Arabidopsis thaliana | hexanoate | 2.67 | 0.58 | 4.62 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant I323A/V399A/W427G, hexanoate |
| acetyl-CoA synthetase (atACS)variant I323A/V399A/W427G | Arabidopsis thaliana | octanoate | 6.1 | 4.1 | 1.47 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant I323A/V399A/W427G, octanoate |
| acetyl-CoA synthetase (atACS)variant I323A/V399A/W427G | Arabidopsis thaliana | pentanoate | 2.7 | 2.3 | 1.2 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant I323A/V399A/W427G, pentanoate |
| acetyl-CoA synthetase (atACS)variant I323A/V399A/W427G | Arabidopsis thaliana | propionate | 3.3 | 98 | 0.03 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant I323A/V399A/W427G, propionate |
| acetyl-CoA synthetase (atACS)variant I323A/V399G/W427G | Arabidopsis thaliana | 3-methylpentanoate | 2.5 | 3.01 | 0.8 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant I323A/V399G/W427G, 3-methylpentanoate |
| acetyl-CoA synthetase (atACS)variant I323A/V399G/W427G | Arabidopsis thaliana | 4-methylpentanoate | 3 | 1.2 | 2.4 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant I323A/V399G/W427G, 4-methylpentanoate |
| acetyl-CoA synthetase (atACS)variant I323A/V399G/W427G | Arabidopsis thaliana | butyrate | 3.8 | 4.5 | 0.9 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant I323A/V399G/W427G, butyrate |
| acetyl-CoA synthetase (atACS)variant I323A/V399G/W427G | Arabidopsis thaliana | heptanoate | 5.4 | 5 | 1.1 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant I323A/V399G/W427G, heptanoate |
| acetyl-CoA synthetase (atACS)variant I323A/V399G/W427G | Arabidopsis thaliana | hexanoate | 4.8 | 7.8 | 0.62 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant I323A/V399G/W427G, hexanoate |
| acetyl-CoA synthetase (atACS)variant I323A/V399G/W427G | Arabidopsis thaliana | octanoate | 4.5 | 2.4 | 1.9 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant I323A/V399G/W427G, octanoate |
| acetyl-CoA synthetase (atACS)variant I323A/V399G/W427G | Arabidopsis thaliana | pentanoate | 2.2 | 2.1 | 1 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant I323A/V399G/W427G, pentanoate |
| acetyl-CoA synthetase (atACS)variant T324G | Arabidopsis thaliana | 2-methylpropanoate | 0.23 | 0.15 | 1.6 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324G, 2-methylpropanoate |
| acetyl-CoA synthetase (atACS)variant T324G | Arabidopsis thaliana | 3-methylpentanoate | 0.077 | 0.04 | 1.9 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324G, 3-methylpentanoate |
| acetyl-CoA synthetase (atACS)variant T324G | Arabidopsis thaliana | 4-methylpentanoate | 0.17 | 0.07 | 2.4 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324G, 4-methylpentanoate |
| acetyl-CoA synthetase (atACS)variant T324G | Arabidopsis thaliana | acetate | 1.91 | 0.18 | 10.4 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324G, acetate |
| acetyl-CoA synthetase (atACS)variant T324G | Arabidopsis thaliana | butyrate | 0.13 | 0.19 | 0.68 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324G, butyrate |
| acetyl-CoA synthetase (atACS)variant T324G | Arabidopsis thaliana | propionate | 1.78 | 3.2 | 0.56 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324G, propionate |
| acetyl-CoA synthetase (atACS)variant T324G/V399A/W427G | Arabidopsis thaliana | 2-methylpropanoate | 4 | 219 | 0.018 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324G/V399A/W427G, 2-methylpropanoate |
| acetyl-CoA synthetase (atACS)variant T324G/V399A/W427G | Arabidopsis thaliana | 3-methylpentanoate | 7.7 | 2.7 | 2.9 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324G/V399A/W427G, 3-methylpentanoate |
| acetyl-CoA synthetase (atACS)variant T324G/V399A/W427G | Arabidopsis thaliana | 4-methylhexanoateHEPES extraction buffer preparation | 5.9 | 0.25 | 24 calc. | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324G/V399A/W427G, 4-methylhexanoate |
| acetyl-CoA synthetase (atACS)variant T324G/V399A/W427G | Arabidopsis thaliana | 4-methylhexanoateN-terminally His-tagged recombinant full-length atACS | — | — | 2 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324G/V399A/W427G, 4-methylhexanoate |
| acetyl-CoA synthetase (atACS)variant T324G/V399A/W427G | Arabidopsis thaliana | 4-methylpentanoate | 6.6 | 0.22 | 29 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324G/V399A/W427G, 4-methylpentanoate |
| acetyl-CoA synthetase (atACS)variant T324G/V399A/W427G | Arabidopsis thaliana | acetate | 0.6 | 7.8 | 0.07 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324G/V399A/W427G, acetate |
| acetyl-CoA synthetase (atACS)variant T324G/V399A/W427G | Arabidopsis thaliana | adenosine 5'-triphosphate | 5.04 | 0.26 | 19 calc. | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324G/V399A/W427G, adenosine 5'-triphosphate |
| acetyl-CoA synthetase (atACS)variant T324G/V399A/W427G | Arabidopsis thaliana | butyrate | 6.7 | 20 | 0.33 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324G/V399A/W427G, butyrate |
| acetyl-CoA synthetase (atACS)variant T324G/V399A/W427G | Arabidopsis thaliana | coenzyme A | 5.07 | 0.14 | 36 calc. | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324G/V399A/W427G, coenzyme A |
| acetyl-CoA synthetase (atACS)variant T324G/V399A/W427G | Arabidopsis thaliana | heptanoate | 11 | 0.71 | 15 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324G/V399A/W427G, heptanoate |
| acetyl-CoA synthetase (atACS)variant T324G/V399A/W427G | Arabidopsis thaliana | hexanoate | 5.7 | 0.13 | 43 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324G/V399A/W427G, hexanoate |
| acetyl-CoA synthetase (atACS)variant T324G/V399A/W427G | Arabidopsis thaliana | octanoate | 5.2 | 2.1 | 2.4 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324G/V399A/W427G, octanoate |
| acetyl-CoA synthetase (atACS)variant T324G/V399A/W427G | Arabidopsis thaliana | pentanoate | 8.8 | 0.4 | 22 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324G/V399A/W427G, pentanoate |
| acetyl-CoA synthetase (atACS)variant T324G/V399A/W427G | Arabidopsis thaliana | propionate | 3.3 | 98 | 0.03 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324G/V399A/W427G, propionate |
| acetyl-CoA synthetase (atACS)variant T324S/V399A/W427G | Arabidopsis thaliana | 4-methylpentanoate | 8.2 | 1.2 | 6.7 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324S/V399A/W427G, 4-methylpentanoate |
| acetyl-CoA synthetase (atACS)variant T324S/V399A/W427G | Arabidopsis thaliana | butyrate | 19 | 16 | 1.2 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324S/V399A/W427G, butyrate |
| acetyl-CoA synthetase (atACS)variant T324S/V399A/W427G | Arabidopsis thaliana | pentanoate | 7.2 | 1.5 | 4.9 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324S/V399A/W427G, pentanoate |
| acetyl-CoA synthetase (atACS)variant T324V | Arabidopsis thaliana | 2-methylpropanoate | 0.33 | 2.1 | 0.15 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324V, 2-methylpropanoate |
| acetyl-CoA synthetase (atACS)variant T324V | Arabidopsis thaliana | 3-methylpentanoate | 3.9 | 125 | 0.03 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324V, 3-methylpentanoate |
| acetyl-CoA synthetase (atACS)variant T324V | Arabidopsis thaliana | acetate | 0.99 | 0.042 | 24 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324V, acetate |
| acetyl-CoA synthetase (atACS)variant T324V | Arabidopsis thaliana | propionate | 1.1 | 0.62 | 1.8 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant T324V, propionate |
| acetyl-CoA synthetase (atACS)variant V399A | Arabidopsis thaliana | acetate | 3.04 | 3.1 | 0.99 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant V399A, acetate |
| acetyl-CoA synthetase (atACS)variant V399A | Arabidopsis thaliana | adenosine 5'-triphosphate | 2.28 | 0.06 | 38 calc. | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant V399A, adenosine 5'-triphosphate |
| acetyl-CoA synthetase (atACS)variant V399A | Arabidopsis thaliana | coenzyme A | 3.2 | 0.09 | 36 calc. | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant V399A, coenzyme A |
| acetyl-CoA synthetase (atACS)variant V399A | Arabidopsis thaliana | propionate | 2.65 | 3.1 | 0.85 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant V399A, propionate |
| acetyl-CoA synthetase (atACS)variant V399A/W427G | Arabidopsis thaliana | acetate | 5.07 | 44 | 0.11 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant V399A/W427G, acetate |
| acetyl-CoA synthetase (atACS)variant V399A/W427G | Arabidopsis thaliana | adenosine 5'-triphosphate | 6.17 | 0.06 | 100 calc. | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant V399A/W427G, adenosine 5'-triphosphate |
| acetyl-CoA synthetase (atACS)variant V399A/W427G | Arabidopsis thaliana | butyrate | 14.87 | 1.5 | 9.9 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant V399A/W427G, butyrate |
| acetyl-CoA synthetase (atACS)variant V399A/W427G | Arabidopsis thaliana | coenzyme A | 8.04 | 0.09 | 89 calc. | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant V399A/W427G, coenzyme A |
| acetyl-CoA synthetase (atACS)variant V399A/W427G | Arabidopsis thaliana | heptanoate | 9.12 | 5.8 | 1.6 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant V399A/W427G, heptanoate |
| acetyl-CoA synthetase (atACS)variant V399A/W427G | Arabidopsis thaliana | hexanoate | 9.2 | 7.3 | 1.3 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant V399A/W427G, hexanoate |
| acetyl-CoA synthetase (atACS)variant V399A/W427G | Arabidopsis thaliana | octanoate | 3.04 | 6.3 | 0.48 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant V399A/W427G, octanoate |
| acetyl-CoA synthetase (atACS)variant V399A/W427G | Arabidopsis thaliana | pentanoate | 12.25 | 4 | 3.1 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant V399A/W427G, pentanoate |
| acetyl-CoA synthetase (atACS)variant V399A/W427G | Arabidopsis thaliana | propionate | 12.94 | 31 | 0.41 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant V399A/W427G, propionate |
| acetyl-CoA synthetase (atACS)variant W427A | Arabidopsis thaliana | acetate | 11 | 1.9 | 6 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant W427A, acetate |
| acetyl-CoA synthetase (atACS)variant W427A | Arabidopsis thaliana | butyrate | 10.8 | 5.1 | 2.1 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant W427A, butyrate |
| acetyl-CoA synthetase (atACS)variant W427A | Arabidopsis thaliana | propionate | 21 | 23 | 0.9 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant W427A, propionate |
| acetyl-CoA synthetase (atACS)variant W427F | Arabidopsis thaliana | acetate | 11.7 | 1.2 | 15.2 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant W427F, acetate |
| acetyl-CoA synthetase (atACS)variant W427G | Arabidopsis thaliana | acetate | 14.3 | 10.8 | 1.32 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant W427G, acetate |
| acetyl-CoA synthetase (atACS)variant W427G | Arabidopsis thaliana | adenosine 5'-triphosphate | 6.41 | 0.02 | 320 calc. | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant W427G, adenosine 5'-triphosphate |
| acetyl-CoA synthetase (atACS)variant W427G | Arabidopsis thaliana | butyrate | 9.4 | 2.9 | 3.3 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant W427G, butyrate |
| acetyl-CoA synthetase (atACS)variant W427G | Arabidopsis thaliana | coenzyme A | 9.36 | 0.14 | 67 calc. | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant W427G, coenzyme A |
| acetyl-CoA synthetase (atACS)variant W427G | Arabidopsis thaliana | heptanoate | 11 | 10 | 1.1 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant W427G, heptanoate |
| acetyl-CoA synthetase (atACS)variant W427G | Arabidopsis thaliana | hexanoate | 13 | 12.6 | 1.03 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant W427G, hexanoate |
| acetyl-CoA synthetase (atACS)variant W427G | Arabidopsis thaliana | octanoate | 5.8 | 48 | 0.12 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant W427G, octanoate |
| acetyl-CoA synthetase (atACS)variant W427G | Arabidopsis thaliana | pentanoate | 13.8 | 6.5 | 2.13 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant W427G, pentanoate |
| acetyl-CoA synthetase (atACS)variant W427G | Arabidopsis thaliana | propionate | 6.2 | 37.5 | 0.16 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant W427G, propionate |
| acetyl-CoA synthetase (atACS)variant W427H | Arabidopsis thaliana | acetate | 15 | 14 | 1.1 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant W427H, acetate |
| acetyl-CoA synthetase (atACS)variant W427S | Arabidopsis thaliana | 2-methylpropanoate | 1.5 | 1.6 | 1 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant W427S, 2-methylpropanoate |
| acetyl-CoA synthetase (atACS)variant W427S | Arabidopsis thaliana | acetate | 7.2 | 8.9 | 0.81 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant W427S, acetate |
| acetyl-CoA synthetase (atACS)variant W427V | Arabidopsis thaliana | acetate | 10.6 | 10 | 1 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant W427V, acetate |
| acetyl-CoA synthetase (atACS)variant W427V | Arabidopsis thaliana | propionate | 2.5 | 38 | 0.06 | pH 7.5, 37 °C | Open record: acetyl-CoA synthetase (atACS) variant W427V, propionate |