Values CatLog read from this paper

Establishment of mesophilic-like catalytic properties in a thermophilic enzyme without affecting its thermal stability

PMID 31249343doi:10.1038/s41598-019-45560-xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 30 records (52 values) from this paper: 3-isopropylmalate dehydrogenase (TtIPMDH), 3-isopropylmalate dehydrogenase (EcIPMDH) from Thermus thermophilus, Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 22 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.1.1.85

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
3-isopropylmalate dehydrogenase (EcIPMDH)	Escherichia coli	D-3-isopropylmalate	—	0.004	—	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase (EcIPMDH), D-3-isopropylmalate
3-isopropylmalate dehydrogenase (EcIPMDH)	Escherichia coli	D-3-isopropylmalate	—	0.034	—	pH 8, 70 °C	Open record: 3-isopropylmalate dehydrogenase (EcIPMDH), D-3-isopropylmalate
3-isopropylmalate dehydrogenase (EcIPMDH)	Escherichia coli	nicotinamide adenine dinucleotide (NAD+)	13	0.062	210 calc.	pH 8, 25 °C	Open record: 3-isopropylmalate dehydrogenase (EcIPMDH), nicotinamide adenine dinucleotide (NAD+)
3-isopropylmalate dehydrogenase (EcIPMDH)	Escherichia coli	nicotinamide adenine dinucleotide (NAD+)	56	0.17	330 calc.	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase (EcIPMDH), nicotinamide adenine dinucleotide (NAD+)
3-isopropylmalate dehydrogenase (EcIPMDH)	Escherichia coli	nicotinamide adenine dinucleotide (NAD+)	190	1.2	160 calc.	pH 8, 70 °C	Open record: 3-isopropylmalate dehydrogenase (EcIPMDH), nicotinamide adenine dinucleotide (NAD+)
3-isopropylmalate dehydrogenase (TtIPMDH)	Thermus thermophilus	D-3-isopropylmalate	—	0.0023	—	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH), D-3-isopropylmalate
3-isopropylmalate dehydrogenase (TtIPMDH)	Thermus thermophilus	D-3-isopropylmalate	—	0.0037	—	pH 8, 70 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH), D-3-isopropylmalate
3-isopropylmalate dehydrogenase (TtIPMDH)	Thermus thermophilus	nicotinamide adenine dinucleotide (NAD+)	0.37	0.0022	170 calc.	pH 8, 25 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH), nicotinamide adenine dinucleotide (NAD+)
3-isopropylmalate dehydrogenase (TtIPMDH)	Thermus thermophilus	nicotinamide adenine dinucleotide (NAD+)	2.4	0.012	200 calc.	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH), nicotinamide adenine dinucleotide (NAD+)
3-isopropylmalate dehydrogenase (TtIPMDH)	Thermus thermophilus	nicotinamide adenine dinucleotide (NAD+)	79	0.21	380 calc.	pH 8, 70 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH), nicotinamide adenine dinucleotide (NAD+)
3-isopropylmalate dehydrogenase (TtIPMDH)variant A220T	Thermus thermophilus	nicotinamide adenine dinucleotide (NAD+)	0.7	0.0086	81 calc.	pH 8, 25 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH) variant A220T, nicotinamide adenine dinucleotide (NAD+)
3-isopropylmalate dehydrogenase (TtIPMDH)variant A220T/V272A/H273G	Thermus thermophilus	D-3-isopropylmalate	—	0.003	—	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH) variant A220T/V272A/H273G, D-3-isopropylmalate
3-isopropylmalate dehydrogenase (TtIPMDH)variant A220T/V272A/H273G	Thermus thermophilus	D-3-isopropylmalate	—	0.0036	—	pH 8, 70 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH) variant A220T/V272A/H273G, D-3-isopropylmalate
3-isopropylmalate dehydrogenase (TtIPMDH)variant A220T/V272A/H273G	Thermus thermophilus	nicotinamide adenine dinucleotide (NAD+)	4.3	0.1	43 calc.	pH 8, 25 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH) variant A220T/V272A/H273G, nicotinamide adenine dinucleotide (NAD+)
3-isopropylmalate dehydrogenase (TtIPMDH)variant A220T/V272A/H273G	Thermus thermophilus	nicotinamide adenine dinucleotide (NAD+)	16	0.22	73 calc.	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH) variant A220T/V272A/H273G, nicotinamide adenine dinucleotide (NAD+)
3-isopropylmalate dehydrogenase (TtIPMDH)variant A220T/V272A/H273G	Thermus thermophilus	nicotinamide adenine dinucleotide (NAD+)	100	1.6	62 calc.	pH 8, 70 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH) variant A220T/V272A/H273G, nicotinamide adenine dinucleotide (NAD+)
3-isopropylmalate dehydrogenase (TtIPMDH)variant I130C	Thermus thermophilus	nicotinamide adenine dinucleotide (NAD+)	0.66	0.016	41 calc.	pH 8, 25 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH) variant I130C, nicotinamide adenine dinucleotide (NAD+)
3-isopropylmalate dehydrogenase (TtIPMDH)variant I130C/V272A/H273G	Thermus thermophilus	D-3-isopropylmalate	—	0.0017	—	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH) variant I130C/V272A/H273G, D-3-isopropylmalate
3-isopropylmalate dehydrogenase (TtIPMDH)variant I130C/V272A/H273G	Thermus thermophilus	D-3-isopropylmalate	—	0.0033	—	pH 8, 70 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH) variant I130C/V272A/H273G, D-3-isopropylmalate
3-isopropylmalate dehydrogenase (TtIPMDH)variant I130C/V272A/H273G	Thermus thermophilus	nicotinamide adenine dinucleotide (NAD+)	4.2	0.051	82 calc.	pH 8, 25 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH) variant I130C/V272A/H273G, nicotinamide adenine dinucleotide (NAD+)
3-isopropylmalate dehydrogenase (TtIPMDH)variant I130C/V272A/H273G	Thermus thermophilus	nicotinamide adenine dinucleotide (NAD+)	15	0.11	140 calc.	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH) variant I130C/V272A/H273G, nicotinamide adenine dinucleotide (NAD+)
3-isopropylmalate dehydrogenase (TtIPMDH)variant I130C/V272A/H273G	Thermus thermophilus	nicotinamide adenine dinucleotide (NAD+)	80	1.2	67 calc.	pH 8, 70 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH) variant I130C/V272A/H273G, nicotinamide adenine dinucleotide (NAD+)
3-isopropylmalate dehydrogenase (TtIPMDH)variant V272A/H273G	Thermus thermophilus	nicotinamide adenine dinucleotide (NAD+)	3.5	0.027	130 calc.	pH 8, 25 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH) variant V272A/H273G, nicotinamide adenine dinucleotide (NAD+)
3-isopropylmalate dehydrogenase (TtIPMDH)variant mut#11	Thermus thermophilus	nicotinamide adenine dinucleotide (NAD+)	2.9	0.074	39 calc.	pH 8, 25 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH) variant mut#11, nicotinamide adenine dinucleotide (NAD+)
3-isopropylmalate dehydrogenase (TtIPMDH)variant mut#15	Thermus thermophilus	nicotinamide adenine dinucleotide (NAD+)	0.55	0.15	3.7 calc.	pH 8, 25 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH) variant mut#15, nicotinamide adenine dinucleotide (NAD+)
3-isopropylmalate dehydrogenase (TtIPMDH)variant mut#18	Thermus thermophilus	nicotinamide adenine dinucleotide (NAD+)	0.36	0.007	51 calc.	pH 8, 25 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH) variant mut#18, nicotinamide adenine dinucleotide (NAD+)
3-isopropylmalate dehydrogenase (TtIPMDH)variant mut#20	Thermus thermophilus	nicotinamide adenine dinucleotide (NAD+)	0.66	0.092	7.2 calc.	pH 8, 25 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH) variant mut#20, nicotinamide adenine dinucleotide (NAD+)
3-isopropylmalate dehydrogenase (TtIPMDH)variant mut#28	Thermus thermophilus	nicotinamide adenine dinucleotide (NAD+)	3.3	0.56	5.9 calc.	pH 8, 25 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH) variant mut#28, nicotinamide adenine dinucleotide (NAD+)
3-isopropylmalate dehydrogenase (TtIPMDH)variant mut#3	Thermus thermophilus	nicotinamide adenine dinucleotide (NAD+)	1.1	0.019	58 calc.	pH 8, 25 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH) variant mut#3, nicotinamide adenine dinucleotide (NAD+)
3-isopropylmalate dehydrogenase (TtIPMDH)variant mut#5	Thermus thermophilus	nicotinamide adenine dinucleotide (NAD+)	1.3	0.2	6.5 calc.	pH 8, 25 °C	Open record: 3-isopropylmalate dehydrogenase (TtIPMDH) variant mut#5, nicotinamide adenine dinucleotide (NAD+)