Values CatLog read from this paper
Semi-rationally engineered variants of S-adenosylmethionine synthetase from Escherichia coli with reduced product inhibition and improved catalytic activity
PMID 31307578doi:10.1016/j.enzmictec.2019.05.012Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (15 values) from this paper: S-adenosylmethionine synthetase (MAT) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 5 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| S-adenosylmethionine synthetase (MAT) | Escherichia coli | adenosine 5'-triphosphate | — | 0.17 | — | pH 8, 37 °C | Open record: S-adenosylmethionine synthetase (MAT), adenosine 5'-triphosphate |
| S-adenosylmethionine synthetase (MAT) | Escherichia coli | L-methionine | 1.22 | 0.08 | 15 calc. | pH 8, 37 °C | Open record: S-adenosylmethionine synthetase (MAT), L-methionine |
| S-adenosylmethionine synthetase (MAT)variant I303V | Escherichia coli | adenosine 5'-triphosphate | — | 0.1 | — | pH 8, 37 °C | Open record: S-adenosylmethionine synthetase (MAT) variant I303V, adenosine 5'-triphosphate |
| S-adenosylmethionine synthetase (MAT)variant I303V | Escherichia coli | L-methionine | 1.3 | 0.42 | 3.1 calc. | pH 8, 37 °C | Open record: S-adenosylmethionine synthetase (MAT) variant I303V, L-methionine |
| S-adenosylmethionine synthetase (MAT)variant I303V/I65V | Escherichia coli | adenosine 5'-triphosphate | — | 0.05 | — | pH 8, 37 °C | Open record: S-adenosylmethionine synthetase (MAT) variant I303V/I65V, adenosine 5'-triphosphate |
| S-adenosylmethionine synthetase (MAT)variant I303V/I65V | Escherichia coli | L-methionine | 1.94 | 0.43 | 4.5 calc. | pH 8, 37 °C | Open record: S-adenosylmethionine synthetase (MAT) variant I303V/I65V, L-methionine |
| S-adenosylmethionine synthetase (MAT)variant I303V/I65V/L186V | Escherichia coli | adenosine 5'-triphosphate | — | 0.01 | — | pH 8, 37 °C | Open record: S-adenosylmethionine synthetase (MAT) variant I303V/I65V/L186V, adenosine 5'-triphosphate |
| S-adenosylmethionine synthetase (MAT)variant I303V/I65V/L186V | Escherichia coli | L-methionine | 3.28 | 0.3 | 11 calc. | pH 8, 37 °C | Open record: S-adenosylmethionine synthetase (MAT) variant I303V/I65V/L186V, L-methionine |
| S-adenosylmethionine synthetase (MAT)variant I303V/I65V/L186V/N104K | Escherichia coli | adenosine 5'-triphosphate | — | 0.01 | — | pH 8, 37 °C | Open record: S-adenosylmethionine synthetase (MAT) variant I303V/I65V/L186V/N104K, adenosine 5'-triphosphate |
| S-adenosylmethionine synthetase (MAT)variant I303V/I65V/L186V/N104K | Escherichia coli | L-methionine | 4.34 | 0.17 | 26 calc. | pH 8, 37 °C | Open record: S-adenosylmethionine synthetase (MAT) variant I303V/I65V/L186V/N104K, L-methionine |