CatLogenzyme kinetics catalog

Values CatLog read from this paper

Purification and characterization of NADP+-dependent 3 alpha-hydroxysteroid dehydrogenase from mouse liver cytosol

PMID 3170514doi:10.1093/oxfordjournals.jbchem.a122374Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 34 records (34 values) from this paper: NADP+-dependent 3α-hydroxysteroid dehydrogenase from Mus musculus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.50

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus1-Phenyl-1,2-propanedione—0.41—pH 7, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus17α,21-Dihydroxy-5β-pregnane-3,11,20-trione—0.0017—pH 7, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus17β-Hydroxy-5α-androstan-3-one—0.0024—pH 7, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus17β-Hydroxy-5β-androstan-3-one—0.0015—pH 7, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus2,3-Butanedione—0.8—pH 7, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus2,3-Heptanedione—0.077—pH 7, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus2,3-Hexanedione—0.21—pH 7, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus2,3-Pentanedione—0.43—pH 7, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus2-Nitrobenzaldehyde—0.2—pH 7, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus3-Oxo-5β-cholan-24-oic acid—7 × 10⁻⁴—pH 7, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus3α,17α,21-Trihydroxy-5β-pregnane-11,20-dione—0.167—pH 9.5, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus3α,21-Dihydroxy-5β-pregnan-20-one—0.011—pH 9.5, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus3α-hydroxy-5α-androstan-17-one—0.0095—pH 9.5, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus3α-hydroxy-5α-androstan-17-one—0.0091—pH 7, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus3α-Hydroxy-5β-androstan-17-one—0.025—pH 9.5, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus3α-hydroxy-5β-cholan-24-oic acid N-(carboxymethyl)amide—0.0052—pH 9.5, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus3α-Hydroxy-5β-pregnan-20-one—0.0079—pH 9.5, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus4-Nitroacetophenone—0.55—pH 7, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus4-Nitrobenzaldehyde—0.083—pH 7, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus5α-Androstane-3,17-dione—4.9 × 10⁻⁴—pH 7, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus5α-androstane-3,17-dione—4.2 × 10⁻⁴—pH 7, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus5α-Androstane-3α,17β-diol—0.016—pH 9.5, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus5α-Pregnane-2,20-dione—4.8 × 10⁻⁴—pH 7, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus5β-Androstane-3,17-dione—3.5 × 10⁻⁴—pH 7, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus5β-Androstane-3α,17β-diol—0.012—pH 9.5, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculus5β-Pregnane-3,20-dione—3.8 × 10⁻⁴—pH 7, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculusAcenaphthequinone—0.0012—pH 7, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculusBenzene dihydrodiol—6.7—pH 9.5, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculusCamphorquinone—0.031—pH 7, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculusNADP+—0.012—pH 9.5, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculusNADP+—0.0167—pH 7, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculusNADPH100 mM potassium phosphate buffer—9.7 × 10⁻⁴—pH 7, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculusNADPH100 mM potassium phosphate—8.5 × 10⁻⁴—pH 7, 25 °COpen
NADP+-dependent 3α-hydroxysteroid dehydrogenaseMus musculusPyridine-4-aldehyde—0.61—pH 7, 25 °COpen