Values CatLog read from this paper
Structural and Biochemical Investigations of the [4Fe-4S] Cluster-Containing Fumarate Hydratase from Leishmania major
PMID 31743022doi:10.1021/acs.biochem.9b00923Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (48 values) from this paper: LmFH-2, LmFH-1 from Leishmania major.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| LmFH-1 | Leishmania major | (S)-malate | 364 | 13 | 28 | pH 9, 23 °C | Open record: LmFH-1, (S)-malate |
| LmFH-1 | Leishmania major | fumarate | 1159 | 4.2 | 280 | pH 9, 23 °C | Open record: LmFH-1, fumarate |
| LmFH-2 | Leishmania major | (S)-malate | 537 | 11.5 | 47 | pH 9, 23 °C | Open record: LmFH-2, (S)-malate |
| LmFH-2 | Leishmania major | fumarate | 1.5 × 10³ | 4.6 | 330 | pH 9, 23 °C | Open record: LmFH-2, fumarate |
| LmFH-2variant D135A | Leishmania major | (S)-malate | 0.075 | 5.3 | 0.014 | pH 9, 23 °C | Open record: LmFH-2 variant D135A, (S)-malate |
| LmFH-2variant D135A | Leishmania major | fumarate | 0.23 | 1.8 | 0.13 | pH 9, 23 °C | Open record: LmFH-2 variant D135A, fumarate |
| LmFH-2variant H334A | Leishmania major | (S)-malate | 57.6 | 4.6 | 12.5 | pH 9, 23 °C | Open record: LmFH-2 variant H334A, (S)-malate |
| LmFH-2variant H334A | Leishmania major | fumarate | 196 | 4.2 | 47 | pH 9, 23 °C | Open record: LmFH-2 variant H334A, fumarate |
| LmFH-2variant R173A | Leishmania major | (S)-malate | 0.033 | 4.7 | 0.007 | pH 9, 23 °C | Open record: LmFH-2 variant R173A, (S)-malate |
| LmFH-2variant R173A | Leishmania major | fumarate | 0.15 | 2.6 | 0.06 | pH 9, 23 °C | Open record: LmFH-2 variant R173A, fumarate |
| LmFH-2variant R421A | Leishmania major | (S)-malate | 0.144 | 4.1 | 0.035 | pH 9, 23 °C | Open record: LmFH-2 variant R421A, (S)-malate |
| LmFH-2variant R421A | Leishmania major | fumarate | 0.72 | 4.9 | 0.15 | pH 9, 23 °C | Open record: LmFH-2 variant R421A, fumarate |
| LmFH-2variant R471A | Leishmania major | (S)-malate | 0.038 | 4.3 | 0.009 | pH 9, 23 °C | Open record: LmFH-2 variant R471A, (S)-malate |
| LmFH-2variant R471A | Leishmania major | fumarate | 0.12 | 2.3 | 0.05 | pH 9, 23 °C | Open record: LmFH-2 variant R471A, fumarate |
| LmFH-2variant T467A | Leishmania major | (S)-malate | 0.052 | 4.4 | 0.012 | pH 9, 23 °C | Open record: LmFH-2 variant T467A, (S)-malate |
| LmFH-2variant T467A | Leishmania major | fumarate | 0.2 | 3.1 | 0.06 | pH 9, 23 °C | Open record: LmFH-2 variant T467A, fumarate |