Values CatLog read from this paper

Opposites Attract: Escherichia coli Heptosyltransferase I Conformational Changes Induced by Interactions between the Substrate and Positively Charged Residues

PMID 32011131doi:10.1021/acs.biochem.9b01005Open in Browse

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CatLog read 17 records (51 values) from this paper: heptosyltransferase I (HepI) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.4.99.23

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
heptosyltransferase I (HepI)	Escherichia coli	O-deacylated Kdo2-lipid A	0.36	0.0029	124	pH 7.5, 37 °C	Open record: heptosyltransferase I (HepI), O-deacylated Kdo2-lipid A
heptosyltransferase I (HepI)variant K192A	Escherichia coli	O-deacylated Kdo2-lipid A	0.14	0.014	11	pH 7.5, 37 °C	Open record: heptosyltransferase I (HepI) variant K192A, O-deacylated Kdo2-lipid A
heptosyltransferase I (HepI)variant K64A	Escherichia coli	O-deacylated Kdo2-lipid A	0.33	0.008	41	pH 7.5, 37 °C	Open record: heptosyltransferase I (HepI) variant K64A, O-deacylated Kdo2-lipid A
heptosyltransferase I (HepI)variant K64A/R63A	Escherichia coli	O-deacylated Kdo2-lipid A	0.25	0.04	7	pH 7.5, 37 °C	Open record: heptosyltransferase I (HepI) variant K64A/R63A, O-deacylated Kdo2-lipid A
heptosyltransferase I (HepI)variant K64A/R63A/R60A	Escherichia coli	O-deacylated Kdo2-lipid A	0.2	0.02	10	pH 7.5, 37 °C	Open record: heptosyltransferase I (HepI) variant K64A/R63A/R60A, O-deacylated Kdo2-lipid A
heptosyltransferase I (HepI)variant K64A/R63A/R61A	Escherichia coli	O-deacylated Kdo2-lipid A	0.21	0.037	6	pH 7.5, 37 °C	Open record: heptosyltransferase I (HepI) variant K64A/R63A/R61A, O-deacylated Kdo2-lipid A
heptosyltransferase I (HepI)variant K64A/R63A/R61A/R60A	Escherichia coli	O-deacylated Kdo2-lipid A	0.28	0.056	5	pH 7.5, 37 °C	Open record: heptosyltransferase I (HepI) variant K64A/R63A/R61A/R60A, O-deacylated Kdo2-lipid A
heptosyltransferase I (HepI)variant K98A	Escherichia coli	O-deacylated Kdo2-lipid A	0.21	0.016	13.1	pH 7.5, 37 °C	Open record: heptosyltransferase I (HepI) variant K98A, O-deacylated Kdo2-lipid A
heptosyltransferase I (HepI)variant R120A	Escherichia coli	O-deacylated Kdo2-lipid A	0.32	0.015	21	pH 7.5, 37 °C	Open record: heptosyltransferase I (HepI) variant R120A, O-deacylated Kdo2-lipid A
heptosyltransferase I (HepI)variant R120A/K64A	Escherichia coli	O-deacylated Kdo2-lipid A	0.47	0.034	14	pH 7.5, 37 °C	Open record: heptosyltransferase I (HepI) variant R120A/K64A, O-deacylated Kdo2-lipid A
heptosyltransferase I (HepI)variant R120A/R60A	Escherichia coli	O-deacylated Kdo2-lipid A	0.281	0.0014	200	pH 7.5, 37 °C	Open record: heptosyltransferase I (HepI) variant R120A/R60A, O-deacylated Kdo2-lipid A
heptosyltransferase I (HepI)variant R120A/R61A	Escherichia coli	O-deacylated Kdo2-lipid A	0.26	0.0029	90	pH 7.5, 37 °C	Open record: heptosyltransferase I (HepI) variant R120A/R61A, O-deacylated Kdo2-lipid A
heptosyltransferase I (HepI)variant R120A/R63A	Escherichia coli	O-deacylated Kdo2-lipid A	0.37	0.011	30	pH 7.5, 37 °C	Open record: heptosyltransferase I (HepI) variant R120A/R63A, O-deacylated Kdo2-lipid A
heptosyltransferase I (HepI)variant R189A	Escherichia coli	O-deacylated Kdo2-lipid A	0.116	0.006	19	pH 7.5, 37 °C	Open record: heptosyltransferase I (HepI) variant R189A, O-deacylated Kdo2-lipid A
heptosyltransferase I (HepI)variant R60A	Escherichia coli	O-deacylated Kdo2-lipid A	0.198	0.006	33	pH 7.5, 37 °C	Open record: heptosyltransferase I (HepI) variant R60A, O-deacylated Kdo2-lipid A
heptosyltransferase I (HepI)variant R61A	Escherichia coli	O-deacylated Kdo2-lipid A	0.36	0.0025	140	pH 7.5, 37 °C	Open record: heptosyltransferase I (HepI) variant R61A, O-deacylated Kdo2-lipid A
heptosyltransferase I (HepI)variant R63A	Escherichia coli	O-deacylated Kdo2-lipid A	0.41	0.01	41	pH 7.5, 37 °C	Open record: heptosyltransferase I (HepI) variant R63A, O-deacylated Kdo2-lipid A