Values CatLog read from this paper
Probing remote residues important for catalysis in Escherichia coli ornithine transcarbamoylase
PMID 32027716doi:10.1371/journal.pone.0228487Open in Browse
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CatLog read 30 records (90 values) from this paper: ornithine transcarbamoylase (anabolic OTC) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| ornithine transcarbamoylase (anabolic OTC) | Escherichia coli | carbamoyl phosphate | 410 | 0.27 | 1.5 × 10³ | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC), carbamoyl phosphate |
| ornithine transcarbamoylase (anabolic OTC) | Escherichia coli | L-ornithine | 440 | 0.53 | 830 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC), L-ornithine |
| ornithine transcarbamoylase (anabolic OTC)variant C273A | Escherichia coli | carbamoyl phosphate | 140 | 0.26 | 560 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant C273A, carbamoyl phosphate |
| ornithine transcarbamoylase (anabolic OTC)variant C273A | Escherichia coli | L-ornithine | 170 | 0.73 | 260 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant C273A, L-ornithine |
| ornithine transcarbamoylase (anabolic OTC)variant D140N | Escherichia coli | carbamoyl phosphate | 360 | 1.4 | 290 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant D140N, carbamoyl phosphate |
| ornithine transcarbamoylase (anabolic OTC)variant D140N | Escherichia coli | L-ornithine | 460 | 17 | 30 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant D140N, L-ornithine |
| ornithine transcarbamoylase (anabolic OTC)variant D231A | Escherichia coli | carbamoyl phosphate | 0.48 | 0.15 | 3.4 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant D231A, carbamoyl phosphate |
| ornithine transcarbamoylase (anabolic OTC)variant D231A | Escherichia coli | L-ornithine | 0.7 | 0.59 | 1.4 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant D231A, L-ornithine |
| ornithine transcarbamoylase (anabolic OTC)variant E299D | Escherichia coli | carbamoyl phosphate | 120 | 0.22 | 620 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant E299D, carbamoyl phosphate |
| ornithine transcarbamoylase (anabolic OTC)variant E299D | Escherichia coli | L-ornithine | 170 | 0.9 | 200 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant E299D, L-ornithine |
| ornithine transcarbamoylase (anabolic OTC)variant E299Q | Escherichia coli | carbamoyl phosphate | 4.6 | 0.34 | 14 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant E299Q, carbamoyl phosphate |
| ornithine transcarbamoylase (anabolic OTC)variant E299Q | Escherichia coli | L-ornithine | 5.1 | 0.33 | 16 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant E299Q, L-ornithine |
| ornithine transcarbamoylase (anabolic OTC)variant H272L | Escherichia coli | carbamoyl phosphate | 4.2 | 0.93 | 5 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant H272L, carbamoyl phosphate |
| ornithine transcarbamoylase (anabolic OTC)variant H272L | Escherichia coli | L-ornithine | 4.3 | 0.68 | 7.1 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant H272L, L-ornithine |
| ornithine transcarbamoylase (anabolic OTC)variant H272N | Escherichia coli | carbamoyl phosphate | 180 | 0.5 | 360 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant H272N, carbamoyl phosphate |
| ornithine transcarbamoylase (anabolic OTC)variant H272N | Escherichia coli | L-ornithine | 95 | 0.4 | 240 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant H272N, L-ornithine |
| ornithine transcarbamoylase (anabolic OTC)variant Q104L | Escherichia coli | carbamoyl phosphate | 88 | 0.4 | 260 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant Q104L, carbamoyl phosphate |
| ornithine transcarbamoylase (anabolic OTC)variant Q104L | Escherichia coli | L-ornithine | 52 | 0.81 | 80 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant Q104L, L-ornithine |
| ornithine transcarbamoylase (anabolic OTC)variant R57A | Escherichia coli | carbamoyl phosphate | 7.5 | 0.28 | 27 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant R57A, carbamoyl phosphate |
| ornithine transcarbamoylase (anabolic OTC)variant R57A | Escherichia coli | L-ornithine | 8.2 | 0.44 | 19 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant R57A, L-ornithine |
| ornithine transcarbamoylase (anabolic OTC)variant S61A | Escherichia coli | carbamoyl phosphate | 74 | 0.3 | 250 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant S61A, carbamoyl phosphate |
| ornithine transcarbamoylase (anabolic OTC)variant S61A | Escherichia coli | L-ornithine | 240 | 0.53 | 460 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant S61A, L-ornithine |
| ornithine transcarbamoylase (anabolic OTC)variant Y160F | Escherichia coli | carbamoyl phosphate | 120 | 0.53 | 230 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant Y160F, carbamoyl phosphate |
| ornithine transcarbamoylase (anabolic OTC)variant Y160F | Escherichia coli | L-ornithine | 110 | 1 | 100 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant Y160F, L-ornithine |
| ornithine transcarbamoylase (anabolic OTC)variant Y160S | Escherichia coli | carbamoyl phosphate | 300 | 0.3 | 1 × 10³ | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant Y160S, carbamoyl phosphate |
| ornithine transcarbamoylase (anabolic OTC)variant Y160S | Escherichia coli | L-ornithine | 410 | 7.3 | 61 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant Y160S, L-ornithine |
| ornithine transcarbamoylase (anabolic OTC)variant Y229F | Escherichia coli | carbamoyl phosphate | 150 | 0.33 | 440 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant Y229F, carbamoyl phosphate |
| ornithine transcarbamoylase (anabolic OTC)variant Y229F | Escherichia coli | L-ornithine | 130 | 0.45 | 310 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant Y229F, L-ornithine |
| ornithine transcarbamoylase (anabolic OTC)variant Y229S | Escherichia coli | carbamoyl phosphate | 290 | 0.32 | 920 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant Y229S, carbamoyl phosphate |
| ornithine transcarbamoylase (anabolic OTC)variant Y229S | Escherichia coli | L-ornithine | 350 | 0.61 | 590 | pH 8.5, 20 °C | Open record: ornithine transcarbamoylase (anabolic OTC) variant Y229S, L-ornithine |