Values CatLog read from this paper
Human aminolevulinate synthase structure reveals a eukaryotic-specific autoinhibitory loop regulating substrate binding and product release
PMID 32499479doi:10.1038/s41467-020-16586-xOpen in Browse
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CatLog read 17 records (41 values) from this paper: erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2) from Homo sapiens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2) | Homo sapiens | glycinehsALAS2ΔN142; residues 143-587; tag-free (His6-tag removed) | — | 5.7 | 0.0087 | pH 7.4, 37 °C | Open record: erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2), glycine |
| erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2) | Homo sapiens | glycinehsALAS2ΔN142; residues 143-587; N-terminal His6-tagged | — | 5 | 0.0107 | pH 7.4, 37 °C | Open record: erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2), glycine |
| erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2) | Homo sapiens | glycinehsALAS2ΔN78; residues 79-587; N-terminal His6-tagged | — | 8.5 | 0.0068 | pH 7.4, 37 °C | Open record: erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2), glycine |
| erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2) | Homo sapiens | succinyl-coenzyme AhsALAS2ΔN142; residues 143-587; tag-free (His6-tag removed) | 0.05 | 0.039 | 1.25 | pH 7.4, 37 °C | Open record: erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2), succinyl-coenzyme A |
| erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2) | Homo sapiens | succinyl-coenzyme AhsALAS2ΔN142; residues 143-587; N-terminal His6-tagged | 0.053 | 0.042 | 1.27 | pH 7.4, 37 °C | Open record: erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2), succinyl-coenzyme A |
| erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2) | Homo sapiens | succinyl-coenzyme AhsALAS2ΔN78; residues 79-587; N-terminal His6-tagged | 0.058 | 0.032 | 1.83 | pH 7.4, 37 °C | Open record: erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2), succinyl-coenzyme A |
| erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2) | Homo sapiens | succinyl-coenzyme AN-terminally truncated hsALAS2 expressed in Sf9 insect cells | — | 0.031 | — | pH 7.4, 37 °C | Open record: erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2), succinyl-coenzyme A |
| erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2)variant Q548X | Homo sapiens | glycine | — | 12.4 | 0.0233 | pH 7.4, 37 °C | Open record: erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2) variant Q548X, glycine |
| erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2)variant Q548X | Homo sapiens | succinyl-coenzyme A | 0.288 | 0.055 | 5.2 | pH 7.4, 37 °C | Open record: erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2) variant Q548X, succinyl-coenzyme A |
| erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2)variant R511E | Homo sapiens | glycinetag-free (His6-tag removed) | — | 26 | 0.004 | pH 7.4, 37 °C | Open record: erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2) variant R511E, glycine |
| erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2)variant R511E | Homo sapiens | glycineN-terminal His6-tagged | — | 22 | 0.0048 | pH 7.4, 37 °C | Open record: erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2) variant R511E, glycine |
| erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2)variant R511E | Homo sapiens | succinyl-coenzyme Atag-free (His6-tag removed) | 0.105 | 0.053 | 2 | pH 7.4, 37 °C | Open record: erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2) variant R511E, succinyl-coenzyme A |
| erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2)variant R511E | Homo sapiens | succinyl-coenzyme AN-terminal His6-tagged | 0.107 | 0.043 | 2.5 | pH 7.4, 37 °C | Open record: erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2) variant R511E, succinyl-coenzyme A |
| erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2)variant delAT | Homo sapiens | glycine | — | 10.3 | 0.025 | pH 7.4, 37 °C | Open record: erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2) variant delAT, glycine |
| erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2)variant delAT | Homo sapiens | succinyl-coenzyme A | 0.257 | 0.056 | 4.7 | pH 7.4, 37 °C | Open record: erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2) variant delAT, succinyl-coenzyme A |
| erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2)variant delG | Homo sapiens | glycine | — | 6.7 | 0.011 | pH 7.4, 37 °C | Open record: erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2) variant delG, glycine |
| erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2)variant delG | Homo sapiens | succinyl-coenzyme A | 0.075 | 0.02 | 3.8 | pH 7.4, 37 °C | Open record: erythroid-specific 5'-aminolevulinate synthase ALAS2 (hsALAS2) variant delG, succinyl-coenzyme A |