Values CatLog read from this paper

Ancestral sequence reconstruction produces thermally stable enzymes with mesophilic enzyme-like catalytic properties

PMID 32968141doi:10.1038/s41598-020-72418-4Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 26 records (42 values) from this paper: 3-isopropylmalate dehydrogenase ancIPMDH-IQ, 3-isopropylmalate dehydrogenase ancIPMDH-ML, 3-isopropylmalate dehydrogenase from Bacillus subtilis, Saccharomyces cerevisiae.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 16 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.1.1.85

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
3-isopropylmalate dehydrogenase	Bacillus subtilis	D-3-isopropylmalate	—	7.8 × 10⁻⁴	—	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase, D-3-isopropylmalate
3-isopropylmalate dehydrogenase	Bacillus subtilis	nicotinamide adenine dinucleotide (oxidized)	2.8	0.11	25 calc.	pH 8, 25 °C	Open record: 3-isopropylmalate dehydrogenase, nicotinamide adenine dinucleotide (oxidized)
3-isopropylmalate dehydrogenase	Bacillus subtilis	nicotinamide adenine dinucleotide (oxidized)	9.4	0.42	22 calc.	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase, nicotinamide adenine dinucleotide (oxidized)
3-isopropylmalate dehydrogenase	Saccharomyces cerevisiae	D-3-isopropylmalate	—	0.0045	—	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase, D-3-isopropylmalate
3-isopropylmalate dehydrogenase	Saccharomyces cerevisiae	nicotinamide adenine dinucleotide (oxidized)	5.1	0.13	39 calc.	pH 8, 25 °C	Open record: 3-isopropylmalate dehydrogenase, nicotinamide adenine dinucleotide (oxidized)
3-isopropylmalate dehydrogenase	Saccharomyces cerevisiae	nicotinamide adenine dinucleotide (oxidized)	20	0.65	31 calc.	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase, nicotinamide adenine dinucleotide (oxidized)
3-isopropylmalate dehydrogenase ancIPMDH-IQ	—	D-3-isopropylmalate	—	0.0053	—	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase ancIPMDH-IQ, D-3-isopropylmalate
3-isopropylmalate dehydrogenase ancIPMDH-IQ	—	D-3-isopropylmalate	—	0.01	—	pH 8, 70 °C	Open record: 3-isopropylmalate dehydrogenase ancIPMDH-IQ, D-3-isopropylmalate
3-isopropylmalate dehydrogenase ancIPMDH-IQ	—	nicotinamide adenine dinucleotide (oxidized)	1.7	0.045	38 calc.	pH 8, 25 °C	Open record: 3-isopropylmalate dehydrogenase ancIPMDH-IQ, nicotinamide adenine dinucleotide (oxidized)
3-isopropylmalate dehydrogenase ancIPMDH-IQ	—	nicotinamide adenine dinucleotide (oxidized)	4.6	0.067	69 calc.	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase ancIPMDH-IQ, nicotinamide adenine dinucleotide (oxidized)
3-isopropylmalate dehydrogenase ancIPMDH-IQ	—	nicotinamide adenine dinucleotide (oxidized)	22	0.96	23 calc.	pH 8, 70 °C	Open record: 3-isopropylmalate dehydrogenase ancIPMDH-IQ, nicotinamide adenine dinucleotide (oxidized)
3-isopropylmalate dehydrogenase ancIPMDH-IQvariant V277A/H278G	—	D-3-isopropylmalate	—	0.005	—	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase ancIPMDH-IQ variant V277A/H278G, D-3-isopropylmalate
3-isopropylmalate dehydrogenase ancIPMDH-IQvariant V277A/H278G	—	D-3-isopropylmalate	—	0.013	—	pH 8, 70 °C	Open record: 3-isopropylmalate dehydrogenase ancIPMDH-IQ variant V277A/H278G, D-3-isopropylmalate
3-isopropylmalate dehydrogenase ancIPMDH-IQvariant V277A/H278G	—	nicotinamide adenine dinucleotide (oxidized)	2	0.077	26 calc.	pH 8, 25 °C	Open record: 3-isopropylmalate dehydrogenase ancIPMDH-IQ variant V277A/H278G, nicotinamide adenine dinucleotide (oxidized)
3-isopropylmalate dehydrogenase ancIPMDH-IQvariant V277A/H278G	—	nicotinamide adenine dinucleotide (oxidized)	8.5	0.23	37 calc.	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase ancIPMDH-IQ variant V277A/H278G, nicotinamide adenine dinucleotide (oxidized)
3-isopropylmalate dehydrogenase ancIPMDH-IQvariant V277A/H278G	—	nicotinamide adenine dinucleotide (oxidized)	34	4.1	8.3 calc.	pH 8, 70 °C	Open record: 3-isopropylmalate dehydrogenase ancIPMDH-IQ variant V277A/H278G, nicotinamide adenine dinucleotide (oxidized)
3-isopropylmalate dehydrogenase ancIPMDH-ML	—	D-3-isopropylmalate	—	0.0047	—	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase ancIPMDH-ML, D-3-isopropylmalate
3-isopropylmalate dehydrogenase ancIPMDH-ML	—	D-3-isopropylmalate	—	0.01	—	pH 8, 70 °C	Open record: 3-isopropylmalate dehydrogenase ancIPMDH-ML, D-3-isopropylmalate
3-isopropylmalate dehydrogenase ancIPMDH-ML	—	nicotinamide adenine dinucleotide (oxidized)	1.2	0.037	32 calc.	pH 8, 25 °C	Open record: 3-isopropylmalate dehydrogenase ancIPMDH-ML, nicotinamide adenine dinucleotide (oxidized)
3-isopropylmalate dehydrogenase ancIPMDH-ML	—	nicotinamide adenine dinucleotide (oxidized)	4.3	0.054	80 calc.	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase ancIPMDH-ML, nicotinamide adenine dinucleotide (oxidized)
3-isopropylmalate dehydrogenase ancIPMDH-ML	—	nicotinamide adenine dinucleotide (oxidized)	22	0.73	30 calc.	pH 8, 70 °C	Open record: 3-isopropylmalate dehydrogenase ancIPMDH-ML, nicotinamide adenine dinucleotide (oxidized)
3-isopropylmalate dehydrogenase ancIPMDH-MLvariant V277A/H278G	—	D-3-isopropylmalate	—	0.005	—	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase ancIPMDH-ML variant V277A/H278G, D-3-isopropylmalate
3-isopropylmalate dehydrogenase ancIPMDH-MLvariant V277A/H278G	—	D-3-isopropylmalate	—	0.011	—	pH 8, 70 °C	Open record: 3-isopropylmalate dehydrogenase ancIPMDH-ML variant V277A/H278G, D-3-isopropylmalate
3-isopropylmalate dehydrogenase ancIPMDH-MLvariant V277A/H278G	—	nicotinamide adenine dinucleotide (oxidized)	1.6	0.033	48 calc.	pH 8, 25 °C	Open record: 3-isopropylmalate dehydrogenase ancIPMDH-ML variant V277A/H278G, nicotinamide adenine dinucleotide (oxidized)
3-isopropylmalate dehydrogenase ancIPMDH-MLvariant V277A/H278G	—	nicotinamide adenine dinucleotide (oxidized)	5	0.24	21 calc.	pH 8, 40 °C	Open record: 3-isopropylmalate dehydrogenase ancIPMDH-ML variant V277A/H278G, nicotinamide adenine dinucleotide (oxidized)
3-isopropylmalate dehydrogenase ancIPMDH-MLvariant V277A/H278G	—	nicotinamide adenine dinucleotide (oxidized)	24	3.3	7.3 calc.	pH 8, 70 °C	Open record: 3-isopropylmalate dehydrogenase ancIPMDH-ML variant V277A/H278G, nicotinamide adenine dinucleotide (oxidized)