Values CatLog read from this paper
Enhanced catalytic efficiency and coenzyme affinity of leucine dehydrogenase by comprehensive screening strategy for L-tert-leucine synthesis
PMID 33929595doi:10.1007/s00253-021-11323-wOpen in Browse
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CatLog read 10 records (30 values) from this paper: leucine dehydrogenase (BcLeuDH) from Bacillus cereus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| leucine dehydrogenase (BcLeuDH) | Bacillus cereus | NADH | 33.71 | 0.28 | 118.5 | pH 8.5, 30 °C | Open record: leucine dehydrogenase (BcLeuDH), NADH |
| leucine dehydrogenase (BcLeuDH) | Bacillus cereus | trimethylpyruvate | 94.14 | 60.82 | 1.55 | pH 8.5, 30 °C | Open record: leucine dehydrogenase (BcLeuDH), trimethylpyruvate |
| leucine dehydrogenase (BcLeuDH)variant D126E | Bacillus cereus | NADH | 50.92 | 0.27 | 188.6 | pH 8.5, 30 °C | Open record: leucine dehydrogenase (BcLeuDH) variant D126E, NADH |
| leucine dehydrogenase (BcLeuDH)variant D126E | Bacillus cereus | trimethylpyruvate | 77.36 | 23.17 | 3.34 | pH 8.5, 30 °C | Open record: leucine dehydrogenase (BcLeuDH) variant D126E, trimethylpyruvate |
| leucine dehydrogenase (BcLeuDH)variant E116V | Bacillus cereus | NADH | 60.48 | 0.25 | 241.9 | pH 8.5, 30 °C | Open record: leucine dehydrogenase (BcLeuDH) variant E116V, NADH |
| leucine dehydrogenase (BcLeuDH)variant E116V | Bacillus cereus | trimethylpyruvate | 60.49 | 10.74 | 5.63 | pH 8.5, 30 °C | Open record: leucine dehydrogenase (BcLeuDH) variant E116V, trimethylpyruvate |
| leucine dehydrogenase (BcLeuDH)variant E24A/D126E | Bacillus cereus | NADH | 42.62 | 0.154 | 276.8 | pH 8.5, 30 °C | Open record: leucine dehydrogenase (BcLeuDH) variant E24A/D126E, NADH |
| leucine dehydrogenase (BcLeuDH)variant E24A/D126E | Bacillus cereus | trimethylpyruvate | 93.27 | 17.57 | 5.31 | pH 8.5, 30 °C | Open record: leucine dehydrogenase (BcLeuDH) variant E24A/D126E, trimethylpyruvate |
| leucine dehydrogenase (BcLeuDH)variant E24V/E116V | Bacillus cereus | NADH | 35.09 | 0.1 | 350.9 | pH 8.5, 30 °C | Open record: leucine dehydrogenase (BcLeuDH) variant E24V/E116V, NADH |
| leucine dehydrogenase (BcLeuDH)variant E24V/E116V | Bacillus cereus | trimethylpyruvate | 66.21 | 7.92 | 8.36 | pH 8.5, 30 °C | Open record: leucine dehydrogenase (BcLeuDH) variant E24V/E116V, trimethylpyruvate |