CatLogenzyme kinetics catalog

Values CatLog read from this paper

Biochemical elucidation of citrate accumulation in Synechocystis sp. PCC 6803 via kinetic analysis of aconitase

PMID 34429477doi:10.1038/s41598-021-96432-2Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 30 records (68 values) from this paper: aconitase B (SyAcnB) from Synechocystis sp. PCC 6803.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 4.2.1.3

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateenzyme reactivated with 5 mM DTT; optimal conditions9.121.138.11pH 7.7, 45 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrate7.280.6810.68pH 7, 30 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrate70.88.79pH 8, 30 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrate4.422.281.95pH 9, 30 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateenzyme reactivated with 5 mM DTT; repeated at 45 °C8.670.979.04pH 7, 45 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateenzyme reactivated with 5 mM DTT; repeated at 45 °C8.40.8510.1pH 8, 45 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateenzyme reactivated with 5 mM DTT; repeated at 45 °C5.741.583.69pH 9, 45 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateenzyme reactivated with 5 mM DTT; GST fusion; in the text—0.7411.65pH 7.7, 45 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateenzyme reactivated with 5 mM DTT; GST fusion; in the text—3.013.76pH 7.7, 45 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateenzyme reactivated with 5 mM DTT; with 1 mM Ca2+ (CaCl2)—2.614.01pH 7.7, 45 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateGST-SyAcnB reactivated with 5 mM DTT; 100 µM Na2S; fusion9.12—8.11pH 7.7, 45 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateGST-SyAcnB reactivated with 5 mM DTT; 100 µM Na2S; fusion—0.97—pH 7, 45 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateGST-SyAcnB reactivated with 5 mM DTT; 100 µM Na2S; fusion—0.85—pH 8, 45 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateGST-SyAcnB reactivated with 5 mM DTT; 100 µM Na2S; fusion—1.58—pH 9, 45 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateGST-SyAcnB reactivated with 5 mM DTT; 100 µM Na2S; fusion—1.148.81pH 7.7, 45 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateGST-SyAcnB reactivated with 5 mM DTT; 100 µM Na2S; fusion—1.825.06pH 7.7, 45 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803DL-isocitrate16.7—10.88pH 8, 53 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803DL-isocitrate—0.36—pH 7, 45 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803DL-isocitrate—0.72—pH 8, 45 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803DL-isocitrate—3.79—pH 9, 45 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803DL-isocitratewith 1 mM 2-OG (2-oxoglutarate) added—1.0814.36pH 8, 53 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803DL-isocitratewith 5 mM 2-OG (2-oxoglutarate) added—1.3610pH 8, 53 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803isocitrateoptimal conditions16.71.5410.88pH 8, 53 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803isocitrate6.360.2130.17pH 7, 30 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803isocitrate9.020.4719.13pH 8, 30 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803isocitrate5.191.653.18pH 9, 30 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803isocitrate8.160.3622.7pH 7, 45 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803isocitrate12.450.7217.41pH 8, 45 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803isocitrate9.253.792.54pH 9, 45 °COpen
aconitase B (SyAcnB)Synechocystis sp. PCC 6803isocitrate—0.9114.5pH 8, 53 °COpen