CatLogenzyme kinetics catalog

EC 4.2.1.3: aconitate hydratase

LyasesOpen in Browse

CatLog read 64 records for EC 4.2.1.3 from 5 papers, for 4 organisms. Names in these papers: aconitase B (SyAcnB), aconitase B (AcnB), aconitase A (AcnA), aconitase.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 51 records for EC 4.2.1.3 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.2.1.3 in Browse.

Papers

PaperOrganismsRecords
Biochemical elucidation of citrate accumulation in Synechocystis sp. PCC 6803 via kinetic analysis of aconitaseSynechocystis sp. PCC 680330
Biochemical and spectroscopic characterization of Escherichia coli aconitases (AcnA and AcnB)Escherichia coli24
Purification and characterization of the first archaeal aconitase from the thermoacidophilic Sulfolobus acidocaldariusSulfolobus acidocaldarius5
Biochemical characterisation of aconitase from Corynebacterium glutamicumCorynebacterium glutamicum3
Kinetic compartmentalization by unnatural reaction for itaconate productionEscherichia coli2

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
aconitaseCorynebacterium glutamicumcis-aconitate710.01853.84 × 10³pH 8, 30 °CPMID 20647021Open
aconitaseCorynebacterium glutamicumcitrate19.60.4840.8pH 8, 30 °CPMID 20647021Open
aconitaseCorynebacterium glutamicumD,L-isocitrate28.90.55252.4pH 8, 30 °CPMID 20647021Open
aconitaseSulfolobus acidocaldariuscis-aconitate—0.108—pH 7.4, 75 °CPMID 11248696Open
aconitaseSulfolobus acidocaldariuscis-aconitate—0.053—pH 8.5, 70 °CPMID 11248696Open
aconitaseSulfolobus acidocaldariuscitrate—2.9—pH 7.4, 75 °CPMID 11248696Open
aconitaseSulfolobus acidocaldariuscitrate—0.358—pH 8.5, 70 °CPMID 11248696Open
aconitaseSulfolobus acidocaldariusisocitrate—0.37—pH 7.4, 75 °CPMID 11248696Open
aconitase A (AcnA)Escherichia colicis-aconitate—0.058—pH 7.4, 20 °CPMID 10585860Open
aconitase A (AcnA)Escherichia colicis-aconitate—0.053—pH 7.4, 20 °CPMID 10585860Open
aconitase A (AcnA)Escherichia colicis-aconitate—0.06—pH 7.4, 20 °CPMID 10585860Open
aconitase A (AcnA)Escherichia colicitrate—1.16—pH 7.4, 20 °CPMID 10585860Open
aconitase A (AcnA)Escherichia colicitrate—1.15—pH 7.4, 20 °CPMID 10585860Open
aconitase A (AcnA)Escherichia colicitrate—1.5—pH 7.4, 20 °CPMID 10585860Open
aconitase A (AcnA)Escherichia coliisocitrate—0.014—pH 7.4, 20 °CPMID 10585860Open
aconitase A (AcnA)Escherichia coliisocitrate—1.77—pH 7.4, 20 °CPMID 10585860Open
aconitase A (AcnA)Escherichia coliisocitratelike another record—0.015—pH 7.4, 20 °CPMID 10585860Open
aconitase A (AcnA)Escherichia coliisocitrate—1.24—pH 7.4, 20 °CPMID 10585860Open
aconitase A (AcnA)Escherichia coliisocitratelike another record—0.015—pH 7.4, 20 °CPMID 10585860Open
aconitase A (AcnA)Escherichia coliisocitrate—1.9—pH 7.4, 20 °CPMID 10585860Open
aconitase B (AcnB)Escherichia colicis-aconitate—0.016—pH 7.4, 20 °CPMID 10585860Open
aconitase B (AcnB)Escherichia colicis-aconitate—0.018—pH 7.4, 20 °CPMID 10585860Open
aconitase B (AcnB)Escherichia colicis-aconitate—0.02—pH 7.4, 20 °CPMID 10585860Open
aconitase B (AcnB)Escherichia colicitrate—11—pH 7.4, 20 °CPMID 10585860Open
aconitase B (AcnB)Escherichia colicitratelike another record—11.2—pH 7.4, 20 °CPMID 10585860Open
aconitase B (AcnB)Escherichia colicitratelike another record—11.2—pH 7.4, 20 °CPMID 10585860Open
aconitase B (AcnB)Escherichia colicitric acid44893.32140337 °CPMID 36097012Open
aconitase B (AcnB)Escherichia coliisocitratelike another record—0.051—pH 7.4, 20 °CPMID 10585860Open
aconitase B (AcnB)Escherichia coliisocitrate—19.7—pH 7.4, 20 °CPMID 10585860Open
aconitase B (AcnB)Escherichia coliisocitrate—0.052—pH 7.4, 20 °CPMID 10585860Open
aconitase B (AcnB)Escherichia coliisocitrate—22.8—pH 7.4, 20 °CPMID 10585860Open
aconitase B (AcnB)Escherichia coliisocitratelike another record—0.051—pH 7.4, 20 °CPMID 10585860Open
aconitase B (AcnB)Escherichia coliisocitrate—24.5—pH 7.4, 20 °CPMID 10585860Open
aconitase B (AcnB)variant W482REscherichia colicitric acid22326.34374.337 °CPMID 36097012Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateenzyme reactivated with 5 mM DTT; optimal conditions9.121.138.11pH 7.7, 45 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrate7.280.6810.68pH 7, 30 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrate70.88.79pH 8, 30 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrate4.422.281.95pH 9, 30 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateenzyme reactivated with 5 mM DTT; repeated at 45 °C8.670.979.04pH 7, 45 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateenzyme reactivated with 5 mM DTT; repeated at 45 °C8.40.8510.1pH 8, 45 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateenzyme reactivated with 5 mM DTT; repeated at 45 °C5.741.583.69pH 9, 45 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateenzyme reactivated with 5 mM DTT; GST fusion; in the text—0.7411.65pH 7.7, 45 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateenzyme reactivated with 5 mM DTT; GST fusion; in the text—3.013.76pH 7.7, 45 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateenzyme reactivated with 5 mM DTT; with 1 mM Ca2+ (CaCl2)—2.614.01pH 7.7, 45 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateGST-SyAcnB reactivated with 5 mM DTT; 100 µM Na2S; fusion9.12—8.11pH 7.7, 45 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateGST-SyAcnB reactivated with 5 mM DTT; 100 µM Na2S; fusion—0.97—pH 7, 45 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateGST-SyAcnB reactivated with 5 mM DTT; 100 µM Na2S; fusion—0.85—pH 8, 45 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateGST-SyAcnB reactivated with 5 mM DTT; 100 µM Na2S; fusion—1.58—pH 9, 45 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateGST-SyAcnB reactivated with 5 mM DTT; 100 µM Na2S; fusion—1.148.81pH 7.7, 45 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803citrateGST-SyAcnB reactivated with 5 mM DTT; 100 µM Na2S; fusion—1.825.06pH 7.7, 45 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803DL-isocitrate16.7—10.88pH 8, 53 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803DL-isocitrate—0.36—pH 7, 45 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803DL-isocitrate—0.72—pH 8, 45 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803DL-isocitrate—3.79—pH 9, 45 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803DL-isocitratewith 1 mM 2-OG (2-oxoglutarate) added—1.0814.36pH 8, 53 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803DL-isocitratewith 5 mM 2-OG (2-oxoglutarate) added—1.3610pH 8, 53 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803isocitrateoptimal conditions16.71.5410.88pH 8, 53 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803isocitrate6.360.2130.17pH 7, 30 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803isocitrate9.020.4719.13pH 8, 30 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803isocitrate5.191.653.18pH 9, 30 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803isocitrate8.160.3622.7pH 7, 45 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803isocitrate12.450.7217.41pH 8, 45 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803isocitrate9.253.792.54pH 9, 45 °CPMID 34429477Open
aconitase B (SyAcnB)Synechocystis sp. PCC 6803isocitrate—0.9114.5pH 8, 53 °CPMID 34429477Open