CatLogenzyme kinetics catalog

Values CatLog read from this paper

Kinetic compartmentalization by unnatural reaction for itaconate production

PMID 36097012doi:10.1038/s41467-022-33033-1Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 14 records (42 values) from this paper: 2-methylcitrate dehydratase (PrpD), aconitase B (AcnB) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 4.2.1.3, EC 4.2.1.79, EC 4.2.1.99

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
2-methylcitrate dehydratase (PrpD)Escherichia coli2-methylcitrate0.0450.960.07537 °COpen
2-methylcitrate dehydratase (PrpD)Escherichia colicis-aconitic acid119.00.7170.637 °COpen
2-methylcitrate dehydratase (PrpD)Escherichia colicitric acid10,56266.39159.437 °COpen
2-methylcitrate dehydratase (PrpD)variant W110V/G111TEscherichia coli2-methylcitrate0.0231.930.01237 °COpen
2-methylcitrate dehydratase (PrpD)variant W110V/G111TEscherichia colicis-aconitic acid172.11.72100.537 °COpen
2-methylcitrate dehydratase (PrpD)variant W110V/G111TEscherichia colicitric acid609314.82410.137 °COpen
2-methylcitrate dehydratase (PrpD)variant W110V/G111T/I331LEscherichia coli2-methylcitrate0.0241.960.01337 °COpen
2-methylcitrate dehydratase (PrpD)variant W110V/G111T/I331LEscherichia colicis-aconitic acid81.830.72113.637 °COpen
2-methylcitrate dehydratase (PrpD)variant W110V/G111T/I331LEscherichia colicitric acid958635.32272.237 °COpen
2-methylcitrate dehydratase (PrpD)variant W110V/I331LEscherichia coli2-methylcitrate0.0351.720.02137 °COpen
2-methylcitrate dehydratase (PrpD)variant W110V/I331LEscherichia colicis-aconitic acid96.341.5263.6337 °COpen
2-methylcitrate dehydratase (PrpD)variant W110V/I331LEscherichia colicitric acid780515.81495.037 °COpen
aconitase B (AcnB)Escherichia colicitric acid44893.32140337 °COpen
aconitase B (AcnB)variant W482REscherichia colicitric acid22326.34374.337 °COpen