Values CatLog read from this paper
Identification of 7 alpha,12 alpha-dihydroxy-5 beta-cholestan-3-one 3 alpha-hydroxysteroid dehydrogenase
PMID 3459552doi:10.1016/0005-2760(86)90131-1Open in Browse
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CatLog read 6 records (18 values) from this paper: 3alpha-hydroxysteroid dehydrogenase (7alpha,12alpha-dihydroxy-5beta-cholestan-3-one 3alpha-reductase) from Rattus norvegicus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 3alpha-hydroxysteroid dehydrogenase (7alpha,12alpha-dihydroxy-5beta-cholestan-3-one 3alpha-reductase) | Rattus norvegicus | 3-oxo-5beta-cholan-24-oic acid | 0.25 | 7 × 10⁻⁴ | 357.2 | pH 7.4 | Open record: 3alpha-hydroxysteroid dehydrogenase (7alpha,12alpha-dihydroxy-5beta-cholestan-3-one 3alpha-reductase), 3-oxo-5beta-cholan-24-oic acid |
| 3alpha-hydroxysteroid dehydrogenase (7alpha,12alpha-dihydroxy-5beta-cholestan-3-one 3alpha-reductase) | Rattus norvegicus | 5beta-cholestane-3alpha,7alpha-diol | 0.062 | 0.0213 | 2.8 | pH 7.4 | Open record: 3alpha-hydroxysteroid dehydrogenase (7alpha,12alpha-dihydroxy-5beta-cholestan-3-one 3alpha-reductase), 5beta-cholestane-3alpha,7alpha-diol |
| 3alpha-hydroxysteroid dehydrogenase (7alpha,12alpha-dihydroxy-5beta-cholestan-3-one 3alpha-reductase) | Rattus norvegicus | 7alpha,12alpha-dihydroxy-5beta-cholestan-3-one | 0.075 | 0.0045 | 16.7 | pH 7.4 | Open record: 3alpha-hydroxysteroid dehydrogenase (7alpha,12alpha-dihydroxy-5beta-cholestan-3-one 3alpha-reductase), 7alpha,12alpha-dihydroxy-5beta-cholestan-3-one |
| 3alpha-hydroxysteroid dehydrogenase (7alpha,12alpha-dihydroxy-5beta-cholestan-3-one 3alpha-reductase) | Rattus norvegicus | 7alpha-hydroxy-5beta-cholestan-3-one100 µM NADPH (100 nmol in 1.0 ml); cosubstrate NADPH | 0.235 | 0.0103 | 22.8 | pH 7.4 | Open record: 3alpha-hydroxysteroid dehydrogenase (7alpha,12alpha-dihydroxy-5beta-cholestan-3-one 3alpha-reductase), 7alpha-hydroxy-5beta-cholestan-3-one |
| 3alpha-hydroxysteroid dehydrogenase (7alpha,12alpha-dihydroxy-5beta-cholestan-3-one 3alpha-reductase) | Rattus norvegicus | 7alpha-hydroxy-5beta-cholestan-3-oneNADH in place of NADPH; cosubstrate NADH | 0.248 | 0.4665 | 0.5 | pH 7.4 | Open record: 3alpha-hydroxysteroid dehydrogenase (7alpha,12alpha-dihydroxy-5beta-cholestan-3-one 3alpha-reductase), 7alpha-hydroxy-5beta-cholestan-3-one |
| 3alpha-hydroxysteroid dehydrogenase (7alpha,12alpha-dihydroxy-5beta-cholestan-3-one 3alpha-reductase) | Rattus norvegicus | Androsterone | 1.078 | 0.0085 | 127 | pH 7.4 | Open record: 3alpha-hydroxysteroid dehydrogenase (7alpha,12alpha-dihydroxy-5beta-cholestan-3-one 3alpha-reductase), Androsterone |