Values CatLog read from this paper
Structural and mechanistic insights into the substrate specificity and hydrolysis of GH31 α-N-acetylgalactosaminidase
PMID 34826537doi:10.1016/j.biochi.2021.11.007Open in Browse
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CatLog read 10 records (30 values) from this paper: EfGH31 (GH31 α-N-acetylgalactosaminidase EfNag31A catalytic domain) from Enterococcus faecalis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| EfGH31 (GH31 α-N-acetylgalactosaminidase EfNag31A catalytic domain)variant D455A | Enterococcus faecalis | 4-nitrophenyl α-N-acetylgalactosaminide | 0.01 | 0.0734 | 0.14 | Open record: EfGH31 (GH31 α-N-acetylgalactosaminidase EfNag31A catalytic domain) variant D455A, 4-nitrophenyl α-N-acetylgalactosaminide | |
| EfGH31 (GH31 α-N-acetylgalactosaminidase EfNag31A catalytic domain)variant D455N | Enterococcus faecalis | 4-nitrophenyl α-N-acetylgalactosaminide | 0.00239 | 0.0232 | 0.1 | Open record: EfGH31 (GH31 α-N-acetylgalactosaminidase EfNag31A catalytic domain) variant D455N, 4-nitrophenyl α-N-acetylgalactosaminide | |
| EfGH31 (GH31 α-N-acetylgalactosaminidase EfNag31A catalytic domain)variant D508N | Enterococcus faecalis | 4-nitrophenyl α-N-acetylgalactosaminide | 0.00231 | 0.0172 | 0.13 | Open record: EfGH31 (GH31 α-N-acetylgalactosaminidase EfNag31A catalytic domain) variant D508N, 4-nitrophenyl α-N-acetylgalactosaminide | |
| EfGH31 (GH31 α-N-acetylgalactosaminidase EfNag31A catalytic domain)variant I542F | Enterococcus faecalis | 4-nitrophenyl α-N-acetylgalactosaminide | 2.04 | 2.23 | 0.92 | Open record: EfGH31 (GH31 α-N-acetylgalactosaminidase EfNag31A catalytic domain) variant I542F, 4-nitrophenyl α-N-acetylgalactosaminide | |
| EfGH31 (GH31 α-N-acetylgalactosaminidase EfNag31A catalytic domain)variant L492R | Enterococcus faecalis | 4-nitrophenyl α-N-acetylgalactosaminide | 0.0175 | 0.0794 | 0.22 | Open record: EfGH31 (GH31 α-N-acetylgalactosaminidase EfNag31A catalytic domain) variant L492R, 4-nitrophenyl α-N-acetylgalactosaminide | |
| EfGH31 (GH31 α-N-acetylgalactosaminidase EfNag31A catalytic domain)variant V456A | Enterococcus faecalis | 4-nitrophenyl α-N-acetylgalactosaminide | 1.4 | 0.101 | 14 | Open record: EfGH31 (GH31 α-N-acetylgalactosaminidase EfNag31A catalytic domain) variant V456A, 4-nitrophenyl α-N-acetylgalactosaminide | |
| EfGH31 (GH31 α-N-acetylgalactosaminidase EfNag31A catalytic domain)variant W221N | Enterococcus faecalis | 4-nitrophenyl α-N-acetylgalactosaminide | 3.79 | 3.86 | 1 | Open record: EfGH31 (GH31 α-N-acetylgalactosaminidase EfNag31A catalytic domain) variant W221N, 4-nitrophenyl α-N-acetylgalactosaminide | |
| EfGH31 (GH31 α-N-acetylgalactosaminidase EfNag31A catalytic domain)variant W570A | Enterococcus faecalis | 4-nitrophenyl α-N-acetylgalactosaminide | 2.89 | 4.44 | 0.65 | Open record: EfGH31 (GH31 α-N-acetylgalactosaminidase EfNag31A catalytic domain) variant W570A, 4-nitrophenyl α-N-acetylgalactosaminide | |
| EfGH31 (GH31 α-N-acetylgalactosaminidase EfNag31A catalytic domain)variant Y386A | Enterococcus faecalis | 4-nitrophenyl α-N-acetylgalactosaminide | 0.0506 | 2.36 | 0.021 | Open record: EfGH31 (GH31 α-N-acetylgalactosaminidase EfNag31A catalytic domain) variant Y386A, 4-nitrophenyl α-N-acetylgalactosaminide | |
| EfGH31 (GH31 α-N-acetylgalactosaminidase EfNag31A catalytic domain)variant Y386F | Enterococcus faecalis | 4-nitrophenyl α-N-acetylgalactosaminide | 0.0851 | 0.06 | 1.4 | Open record: EfGH31 (GH31 α-N-acetylgalactosaminidase EfNag31A catalytic domain) variant Y386F, 4-nitrophenyl α-N-acetylgalactosaminide |