Values CatLog read from this paper
Structural basis for the synthesis of the core 1 structure by C1GalT1
PMID 35504880doi:10.1038/s41467-022-29833-0Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (36 values) from this paper: C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1), DmC1GalT1 (core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase C1GalT1) from Drosophila melanogaster.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1) | Drosophila melanogaster | Ala-Pro-Asp-Ser(alpha-D-GalNAc)-Arg-Pro-NH2 | 0.0318 | 0.2462 | 0.13 | pH 7.5, 37 °C | Open record: C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1), Ala-Pro-Asp-Ser(alpha-D-GalNAc)-Arg-Pro-NH2 |
| C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1) | Drosophila melanogaster | Ala-Pro-Asp-Thr(alpha-D-GalNAc)-Arg-Pro-NH2secreted from HEK293 cells | 0.0588 | 0.195 | 0.3 | pH 7.5, 37 °C | Open record: C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1), Ala-Pro-Asp-Thr(alpha-D-GalNAc)-Arg-Pro-NH2 |
| C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1) | Drosophila melanogaster | Ala-Pro-Asp-Thr(alpha-D-GalNAc)-Arg-Pro-NH2residues S73-Q388; mellitin signal; Endo-H treated | 0.0785 | 0.3226 | 0.243 calc. | pH 7.5, 37 °C | Open record: C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1), Ala-Pro-Asp-Thr(alpha-D-GalNAc)-Arg-Pro-NH2 |
| C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1) | Drosophila melanogaster | Phe-Ala-Ala-Thr(alpha-D-GalNAc)-Gly-Pro-Phe-NH2 | 0.0827 | 0.11 | 0.75 | pH 7.5, 37 °C | Open record: C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1), Phe-Ala-Ala-Thr(alpha-D-GalNAc)-Gly-Pro-Phe-NH2 |
| C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1) | Drosophila melanogaster | Pro-Ala-Ala-Thr(alpha-D-GalNAc)-Gly-Pro-Phe-NH2 | 0.032 | 0.05514 | 0.57 | pH 7.5, 37 °C | Open record: C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1), Pro-Ala-Ala-Thr(alpha-D-GalNAc)-Gly-Pro-Phe-NH2 |
| C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1) | Drosophila melanogaster | Pro-Ala-Ala-Thr(alpha-D-GalNAc)-Gly-Pro-Tyr-NH2 | 0.0565 | 0.05006 | 1.13 | pH 7.5, 37 °C | Open record: C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1), Pro-Ala-Ala-Thr(alpha-D-GalNAc)-Gly-Pro-Tyr-NH2 |
| C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1) | Drosophila melanogaster | Tyr-Ala-Ala-Thr(alpha-D-GalNAc)-Gly-Pro-Phe-NH2 | 0.027 | 0.08302 | 0.33 | pH 7.5, 37 °C | Open record: C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1), Tyr-Ala-Ala-Thr(alpha-D-GalNAc)-Gly-Pro-Phe-NH2 |
| C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1) | Drosophila melanogaster | Tyr-Ala-Ala-Thr(alpha-D-GalNAc)-Gly-Pro-Tyr-NH2 | 0.06 | 0.07817 | 0.77 | pH 7.5, 37 °C | Open record: C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1), Tyr-Ala-Ala-Thr(alpha-D-GalNAc)-Gly-Pro-Tyr-NH2 |
| C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1) | Drosophila melanogaster | Tyr-Ala-Asp-Thr(alpha-D-GalNAc)-Gly-Pro-Phe-NH2 | 0.0668 | 0.1396 | 0.479 calc. | pH 7.5, 37 °C | Open record: C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1), Tyr-Ala-Asp-Thr(alpha-D-GalNAc)-Gly-Pro-Phe-NH2 |
| C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1) | Drosophila melanogaster | Tyr-Ala-Glu-Thr(alpha-D-GalNAc)-Gly-Pro-Phe-NH2 | 0.086 | 0.1188 | 0.72 calc. | pH 7.5, 37 °C | Open record: C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1), Tyr-Ala-Glu-Thr(alpha-D-GalNAc)-Gly-Pro-Phe-NH2 |
| C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1) | Drosophila melanogaster | uridine diphosphate galactoseAPDT*RP fixed at 1 mM; cosubstrate APDT*RP (1000 µM) | 0.0523 | 0.08827 | 0.6 | pH 7.5, 37 °C | Open record: C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1), uridine diphosphate galactose |
| C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1) | Drosophila melanogaster | uridine diphosphate galactoseP4 fixed at 250 µM; cosubstrate P4 (PAAT*GPY, 250 µM) | 0.056 | 0.03533 | 1.58 | pH 7.5, 37 °C | Open record: C1GalT1 (core 1 beta1,3-galactosyltransferase, T-synthase; DmC1GalT1), uridine diphosphate galactose |
| DmC1GalT1 (core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase C1GalT1) | Drosophila melanogaster | Ala-Pro-Asp-Thr(alpha-GalNAc)-Arg-Pro-NH2 (MUC1 glycopeptide, GalNAc alpha-O-linked to Thr) | 0.058 | 0.195 | 0.30 calc. | pH 7.5, 37 °C | Open record: DmC1GalT1 (core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase C1GalT1), Ala-Pro-Asp-Thr(alpha-GalNAc)-Arg-Pro-NH2 (MUC1 glycopeptide, GalNAc alpha-O-linked to Thr) |
| DmC1GalT1 (core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase C1GalT1) | Drosophila melanogaster | uridine diphosphate galactose | — | 0.088 | — | pH 7.5, 37 °C | Open record: DmC1GalT1 (core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase C1GalT1), uridine diphosphate galactose |