CatLogenzyme kinetics catalog

Values CatLog read from this paper

Nucleotide binding as an allosteric regulatory mechanism for Akkermansia muciniphila β-N-acetylhexosaminidase Am2136

PMID 36394293doi:10.1080/19490976.2022.2143221Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 40 records (56 values) from this paper: beta-N-acetylhexosaminidase Am2136 from Akkermansia muciniphila.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.2.1.52

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
beta-N-acetylhexosaminidase Am2136Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminideTable 2—1.46—pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminideTable 2——2.6pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM ATP (allosteric activator); Table 2—0.85—pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM ATP (allosteric activator); Table 2——4.3pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM ADP (allosteric activator); Table 2—0.86—pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM ADP (allosteric activator); Table 2——4.08pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM GTP (allosteric activator); Table 2—0.82—pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM GTP (allosteric activator); Table 2——4.57pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM GDP (allosteric activator); Table 2—0.7—pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM GDP (allosteric activator); Table 2——5.27pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM UTP (allosteric activator); Table 2—0.84—pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM UTP (allosteric activator); Table 2——4.35pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM UDP (allosteric activator); Table 2—0.91—pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM UDP (allosteric activator); Table 2——3.93pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM CTP (allosteric activator); Table 2—0.84—pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM CTP (allosteric activator); Table 2——4.33pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM CDP (allosteric activator); Table 2—0.81—pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM CDP (allosteric activator); Table 2——4.48pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminideEDTA-treated (5 mM EDTA) and dialyzed protein; Table 7—1.53—pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminideEDTA-treated (5 mM EDTA) and dialyzed protein; Table 7——2.58pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminideEDTA-treated (5 mM EDTA) and dialyzed protein; 2 mM MgCl2—1.44—pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminideEDTA-treated (5 mM EDTA) and dialyzed protein; 2 mM MgCl2——2.52pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminideEDTA-treated (5 mM EDTA) and dialyzed protein; 2 mM CaCl2—1.74—pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminideEDTA-treated (5 mM EDTA) and dialyzed protein; 2 mM CaCl2——2.13pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136variant D57A/E59A/Q60AAkkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminide—1.412.55pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136variant E649AAkkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminide—1.452.5pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136variant E649AAkkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM GDP (allosteric activator)—1.392.72pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136variant P595A/H597AAkkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminide—1.582.18pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136variant P595A/H597AAkkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM GDP (allosteric activator)—0.864.33pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136variant R256AAkkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminide—1.492.5pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136variant R256AAkkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM GDP (allosteric activator)—1.083.58pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136variant R592AAkkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminide—1.482.5pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136variant R592AAkkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM GDP (allosteric activator)—0.754.92pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136variant T545A/G546AAkkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminide—3.660.6pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136variant Y327F/Y331FAkkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminide—1.422.55pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136variant Y327F/Y331FAkkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM GDP (allosteric activator)—1.213.25pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136variant Y415AAkkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminide—2.321.43pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136variant Y550AAkkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminide—1.522.38pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136variant Y550AAkkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM GDP (allosteric activator)—1.243.12pH 8, 37 °COpen
beta-N-acetylhexosaminidase Am2136variant ΔD717-K727Akkermansia muciniphila4-nitrophenyl N-acetyl-beta-D-glucosaminide—6.560.18pH 8, 37 °COpen