EC 3.2.1.52: beta-N-acetylhexosaminidase

HydrolasesOpen in Browse

CatLog read 139 records for EC 3.2.1.52 from 11 papers, for 11 organisms. Names in these papers: beta-N-acetylhexosaminidase Am2136, StrH, hexosaminidase D (HexD), β-N-acetylhexosaminidase I (BbhI) and 10 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 196 records for EC 3.2.1.52 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.2.1.52 in Browse.

Papers

Papers with records for EC 3.2.1.52
Paper	Organisms	Records
Nucleotide binding as an allosteric regulatory mechanism for Akkermansia muciniphila β-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	40
Structural basis for the substrate specificity of a novel β-N-acetylhexosaminidase StrH protein from Streptococcus pneumoniae R6	Streptococcus pneumoniae	22
Mechanism of Human Nucleocytoplasmic Hexosaminidase D	Homo sapiens	14
doi:10.1271/bbb1961.51.1679	Bombyx mori	14
Catalytic Efficiency for the Chemoenzymatic Synthesis of Core Human Milk Oligosaccharides and Analogs via Sugar Oxazolines	Bifidobacterium bifidum	12
Role of active-site residues of dispersin B, a biofilm-releasing beta-hexosaminidase from a periodontal pathogen, in substrate hydrolysis	Aggregatibacter actinomycetemcomitans	11
Structural determinants of an insect beta-N-Acetyl-D-hexosaminidase specialized as a chitinolytic enzyme	Ostrinia furnacalis	11
Characterization of glycosyl hydrolase family 3 beta-N-acetylglucosaminidases from Thermotoga maritima and Thermotoga neapolitana	Thermotoga maritima, Thermotoga neapolitana	6
Characterization of a beta-N-acetylhexosaminidase and a beta-N-acetylglucosaminidase/beta-glucosidase from Cellulomonas fimi	Cellulomonas fimi	4
A novel beta-N-acetyl-D-hexosaminidase from the insect Ostrinia furnacalis (Guenée)	Ostrinia furnacalis	3
The identification and molecular characterization of the first archaeal bifunctional exo-β-glucosidase/N-acetyl-β-glucosaminidase demonstrate that family GH116 is made of three functionally distinct…	Sulfolobus solfataricus	2

Records

Records for EC 3.2.1.52 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
beta-N-acetyl-D-hexosaminidase OfHex1	Ostrinia furnacalis	4-nitrophenyl N-acetyl-beta-D-galactosaminide	766	0.43	1805	pH 6.8, 25 °C	PMID 18959754	Open record: beta-N-acetyl-D-hexosaminidase OfHex1, 4-nitrophenyl N-acetyl-beta-D-galactosaminide
beta-N-acetyl-D-hexosaminidase OfHex1	Ostrinia furnacalis	4-nitrophenyl N-acetyl-beta-D-glucosaminide	708	0.29	2482	pH 6.8, 25 °C	PMID 18959754	Open record: beta-N-acetyl-D-hexosaminidase OfHex1, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetyl-D-hexosaminidase OfHex1	Ostrinia furnacalis	N,N'-diacetylchitobiose	934	0.3	3069	25 °C	PMID 18959754	Open record: beta-N-acetyl-D-hexosaminidase OfHex1, N,N'-diacetylchitobiose
beta-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminideTable 2	—	1.46	—	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminideTable 2	—	—	2.6	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM ATP (allosteric activator); Table 2	—	0.85	—	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM ATP (allosteric activator); Table 2	—	—	4.3	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM ADP (allosteric activator); Table 2	—	0.86	—	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM ADP (allosteric activator); Table 2	—	—	4.08	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM GTP (allosteric activator); Table 2	—	0.82	—	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM GTP (allosteric activator); Table 2	—	—	4.57	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM GDP (allosteric activator); Table 2	—	0.7	—	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM GDP (allosteric activator); Table 2	—	—	5.27	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM UTP (allosteric activator); Table 2	—	0.84	—	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM UTP (allosteric activator); Table 2	—	—	4.35	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM UDP (allosteric activator); Table 2	—	0.91	—	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM UDP (allosteric activator); Table 2	—	—	3.93	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM CTP (allosteric activator); Table 2	—	0.84	—	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM CTP (allosteric activator); Table 2	—	—	4.33	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM CDP (allosteric activator); Table 2	—	0.81	—	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM CDP (allosteric activator); Table 2	—	—	4.48	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminideEDTA-treated (5 mM EDTA) and dialyzed protein; Table 7	—	1.53	—	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminideEDTA-treated (5 mM EDTA) and dialyzed protein; Table 7	—	—	2.58	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminideEDTA-treated (5 mM EDTA) and dialyzed protein; 2 mM MgCl2	—	1.44	—	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminideEDTA-treated (5 mM EDTA) and dialyzed protein; 2 mM MgCl2	—	—	2.52	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminideEDTA-treated (5 mM EDTA) and dialyzed protein; 2 mM CaCl2	—	1.74	—	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminideEDTA-treated (5 mM EDTA) and dialyzed protein; 2 mM CaCl2	—	—	2.13	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136variant D57A/E59A/Q60A	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminide	—	1.41	2.55	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136 variant D57A/E59A/Q60A, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136variant E649A	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminide	—	1.45	2.5	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136 variant E649A, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136variant E649A	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM GDP (allosteric activator)	—	1.39	2.72	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136 variant E649A, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136variant P595A/H597A	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminide	—	1.58	2.18	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136 variant P595A/H597A, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136variant P595A/H597A	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM GDP (allosteric activator)	—	0.86	4.33	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136 variant P595A/H597A, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136variant R256A	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminide	—	1.49	2.5	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136 variant R256A, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136variant R256A	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM GDP (allosteric activator)	—	1.08	3.58	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136 variant R256A, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136variant R592A	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminide	—	1.48	2.5	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136 variant R592A, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136variant R592A	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM GDP (allosteric activator)	—	0.75	4.92	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136 variant R592A, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136variant T545A/G546A	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminide	—	3.66	0.6	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136 variant T545A/G546A, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136variant Y327F/Y331F	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminide	—	1.42	2.55	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136 variant Y327F/Y331F, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136variant Y327F/Y331F	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM GDP (allosteric activator)	—	1.21	3.25	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136 variant Y327F/Y331F, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136variant Y415A	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminide	—	2.32	1.43	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136 variant Y415A, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136variant Y550A	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminide	—	1.52	2.38	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136 variant Y550A, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136variant Y550A	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminidewith 1 mM GDP (allosteric activator)	—	1.24	3.12	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136 variant Y550A, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
beta-N-acetylhexosaminidase Am2136variant ΔD717-K727	Akkermansia muciniphila	4-nitrophenyl N-acetyl-beta-D-glucosaminide	—	6.56	0.18	pH 8, 37 °C	PMID 36394293	Open record: beta-N-acetylhexosaminidase Am2136 variant ΔD717-K727, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
CbsA (β-N-acetylglucosaminidase)	Thermotoga neapolitana	p-nitrophenyl di-N-acetyl β-D-chitobiose	220.8	0.44	501.2	pH 7.5, 70 °C	PMID 19914575	Open record: CbsA (β-N-acetylglucosaminidase), p-nitrophenyl di-N-acetyl β-D-chitobiose
CbsA (β-N-acetylglucosaminidase)	Thermotoga neapolitana	p-nitrophenyl N-acetyl β-D-glucosaminide	273.4	0.36	759.4	pH 7.5, 70 °C	PMID 19914575	Open record: CbsA (β-N-acetylglucosaminidase), p-nitrophenyl N-acetyl β-D-glucosaminide
CbsA (β-N-acetylglucosaminidase)	Thermotoga neapolitana	p-nitrophenyl tri-N-acetyl β-D-chitotriose	147.2	0.3	490.7	pH 7.5, 70 °C	PMID 19914575	Open record: CbsA (β-N-acetylglucosaminidase), p-nitrophenyl tri-N-acetyl β-D-chitotriose
dispersin B (DspB)	Aggregatibacter actinomycetemcomitans	4-methylumbelliferyl-beta-D-N-acetylglucosamine	0.0235	0.39	0.0602	pH 5.8, 37 °C	PMID 17949435	Open record: dispersin B (DspB), 4-methylumbelliferyl-beta-D-N-acetylglucosamine
dispersin B (DspB)	Aggregatibacter actinomycetemcomitans	p-nitrophenyl-beta-D-N-acetylglucosamine	0.927	46.2	0.02	pH 5.8, 37 °C	PMID 17949435	Open record: dispersin B (DspB), p-nitrophenyl-beta-D-N-acetylglucosamine
dispersin B (DspB)variant D183N	Aggregatibacter actinomycetemcomitans	p-nitrophenyl-beta-D-N-acetylglucosamine	3.3 × 10⁻⁵	19.4	1.5 × 10⁻⁶	pH 5.8, 37 °C	PMID 17949435	Open record: dispersin B (DspB) variant D183N, p-nitrophenyl-beta-D-N-acetylglucosamine
dispersin B (DspB)variant E184Q	Aggregatibacter actinomycetemcomitans	p-nitrophenyl-beta-D-N-acetylglucosamine	0.00122	3.7	2.8 × 10⁻⁴	pH 5.8, 37 °C	PMID 17949435	Open record: dispersin B (DspB) variant E184Q, p-nitrophenyl-beta-D-N-acetylglucosamine
dispersin B (DspB)variant E332Q	Aggregatibacter actinomycetemcomitans	p-nitrophenyl-beta-D-N-acetylglucosamine	8.3 × 10⁻⁵	6.5	1 × 10⁻⁵	pH 5.8, 37 °C	PMID 17949435	Open record: dispersin B (DspB) variant E332Q, p-nitrophenyl-beta-D-N-acetylglucosamine
dispersin B (DspB)variant R27A	Aggregatibacter actinomycetemcomitans	p-nitrophenyl-beta-D-N-acetylglucosamine	3.8 × 10⁻⁴	14.5	8.3 × 10⁻⁶	pH 5.8, 37 °C	PMID 17949435	Open record: dispersin B (DspB) variant R27A, p-nitrophenyl-beta-D-N-acetylglucosamine
dispersin B (DspB)variant R27K	Aggregatibacter actinomycetemcomitans	p-nitrophenyl-beta-D-N-acetylglucosamine	8.3 × 10⁻⁴	76.2	1.2 × 10⁻⁵	pH 5.8, 37 °C	PMID 17949435	Open record: dispersin B (DspB) variant R27K, p-nitrophenyl-beta-D-N-acetylglucosamine
dispersin B (DspB)variant Y187A	Aggregatibacter actinomycetemcomitans	4-methylumbelliferyl-beta-D-N-acetylglucosamine	0.0072	0.59	0.0122	pH 5.8, 37 °C	PMID 17949435	Open record: dispersin B (DspB) variant Y187A, 4-methylumbelliferyl-beta-D-N-acetylglucosamine
dispersin B (DspB)variant Y187A	Aggregatibacter actinomycetemcomitans	p-nitrophenyl-beta-D-N-acetylglucosamine	0.06	101.1	5 × 10⁻⁴	pH 5.8, 37 °C	PMID 17949435	Open record: dispersin B (DspB) variant Y187A, p-nitrophenyl-beta-D-N-acetylglucosamine
dispersin B (DspB)variant Y278A	Aggregatibacter actinomycetemcomitans	4-methylumbelliferyl-beta-D-N-acetylglucosamine	0.0015	1.13	0.0013	pH 5.8, 37 °C	PMID 17949435	Open record: dispersin B (DspB) variant Y278A, 4-methylumbelliferyl-beta-D-N-acetylglucosamine
dispersin B (DspB)variant Y278A	Aggregatibacter actinomycetemcomitans	p-nitrophenyl-beta-D-N-acetylglucosamine	0.0042	35.9	2.2 × 10⁻⁵	pH 5.8, 37 °C	PMID 17949435	Open record: dispersin B (DspB) variant Y278A, p-nitrophenyl-beta-D-N-acetylglucosamine
Hex20 (beta-N-acetylhexosaminidase, family 20)	Cellulomonas fimi	4'-nitrophenyl beta-N-acetyl-D-galactosaminide	129	0.066	1.95 × 10³	pH 7.08, 25 °C	PMID 16762038	Open record: Hex20 (beta-N-acetylhexosaminidase, family 20), 4'-nitrophenyl beta-N-acetyl-D-galactosaminide
Hex20 (beta-N-acetylhexosaminidase, family 20)	Cellulomonas fimi	4'-nitrophenyl beta-N-acetyl-D-glucosaminide	482	0.053	9.09 × 10³	pH 7.08, 25 °C	PMID 16762038	Open record: Hex20 (beta-N-acetylhexosaminidase, family 20), 4'-nitrophenyl beta-N-acetyl-D-glucosaminide
hexosaminidase D (HexD)	Homo sapiens	3-fluoro-4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside	2.03	0.172	11.78	pH 7.4, 37 °C	PMID 27149221	Open record: hexosaminidase D (HexD), 3-fluoro-4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside
hexosaminidase D (HexD)	Homo sapiens	3-fluoro-4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside	1.54	0.852	1.82	pH 7.4, 37 °C	PMID 27149221	Open record: hexosaminidase D (HexD), 3-fluoro-4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside
hexosaminidase D (HexD)	Homo sapiens	4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranosidesubstrate 15.6 µM-2 mM	1.322	0.0721	18.17	pH 7.4, 37 °C	PMID 27149221	Open record: hexosaminidase D (HexD), 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside
hexosaminidase D (HexD)	Homo sapiens	4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranosidecontinuous assay at 400 nm in 96-well plates; 100 nM HexD	2.42	0.176	13.78	pH 7.4, 37 °C	PMID 27149221	Open record: hexosaminidase D (HexD), 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside
hexosaminidase D (HexD)	Homo sapiens	4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside	0.507	0.607	0.835	pH 7.4, 37 °C	PMID 27149221	Open record: hexosaminidase D (HexD), 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside
hexosaminidase D (HexD)	Homo sapiens	4-nitrophenyl 2-deoxy-2-difluoroacetamido-beta-D-galactopyranoside	0.27	0.102	2.65	pH 7.4, 37 °C	PMID 27149221	Open record: hexosaminidase D (HexD), 4-nitrophenyl 2-deoxy-2-difluoroacetamido-beta-D-galactopyranoside
hexosaminidase D (HexD)	Homo sapiens	4-nitrophenyl 2-deoxy-2-fluoroacetamido-beta-D-galactopyranoside	2.6	0.533	4.98	pH 7.4, 37 °C	PMID 27149221	Open record: hexosaminidase D (HexD), 4-nitrophenyl 2-deoxy-2-fluoroacetamido-beta-D-galactopyranoside
hexosaminidase D (HexD)	Homo sapiens	4-nitrophenyl 2-deoxy-2-trifluoroacetamido-beta-D-galactopyranoside	0.042	0.103	0.408	pH 7.4, 37 °C	PMID 27149221	Open record: hexosaminidase D (HexD), 4-nitrophenyl 2-deoxy-2-trifluoroacetamido-beta-D-galactopyranoside
hexosaminidase D (HexD)variant D148A	Homo sapiens	3-fluoro-4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside	0.00768	0.566	0.01358	pH 7.4, 37 °C	PMID 27149221	Open record: hexosaminidase D (HexD) variant D148A, 3-fluoro-4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside
hexosaminidase D (HexD)variant D148N	Homo sapiens	3-fluoro-4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside	0.00488	1.73	0.00282	pH 7.4, 37 °C	PMID 27149221	Open record: hexosaminidase D (HexD) variant D148N, 3-fluoro-4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside
hexosaminidase D (HexD)variant E149A	Homo sapiens	3-fluoro-4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside	2.93	0.213	13.75	pH 7.4, 37 °C	PMID 27149221	Open record: hexosaminidase D (HexD) variant E149A, 3-fluoro-4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside
hexosaminidase D (HexD)variant E149A	Homo sapiens	4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside	0.602	0.0525	11.52	pH 7.4, 37 °C	PMID 27149221	Open record: hexosaminidase D (HexD) variant E149A, 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside
hexosaminidase D (HexD)variant E149Q	Homo sapiens	4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside	1.488	0.175	8.47	pH 7.4, 37 °C	PMID 27149221	Open record: hexosaminidase D (HexD) variant E149Q, 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside
hexosaminidase D (HexD)variant H92A	Homo sapiens	4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside	0.0515	0.196	0.263	pH 7.4, 37 °C	PMID 27149221	Open record: hexosaminidase D (HexD) variant H92A, 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside
integumental β-N-acetyl-D-glucosaminidase Ex1	Bombyx mori	hexa-N-acetylchitohexaose	55	0.1	530	pH 6, 25 °C	doi:10.1271/bbb1961.51.1679	Open record: integumental β-N-acetyl-D-glucosaminidase Ex1, hexa-N-acetylchitohexaose
integumental β-N-acetyl-D-glucosaminidase Ex1	Bombyx mori	N,N',N'',N'''-tetraacetylchitotetraose	110	0.16	710	pH 6, 25 °C	doi:10.1271/bbb1961.51.1679	Open record: integumental β-N-acetyl-D-glucosaminidase Ex1, N,N',N'',N'''-tetraacetylchitotetraose
integumental β-N-acetyl-D-glucosaminidase Ex1	Bombyx mori	N,N',N''-triacetylchitotriose	130	0.87	1.45 × 10³	pH 6, 25 °C	doi:10.1271/bbb1961.51.1679	Open record: integumental β-N-acetyl-D-glucosaminidase Ex1, N,N',N''-triacetylchitotriose
integumental β-N-acetyl-D-glucosaminidase Ex1	Bombyx mori	N,N'-diacetylchitobiose	620	0.67	930	pH 6, 25 °C	doi:10.1271/bbb1961.51.1679	Open record: integumental β-N-acetyl-D-glucosaminidase Ex1, N,N'-diacetylchitobiose
integumental β-N-acetyl-D-glucosaminidase Ex1	Bombyx mori	p-nitrophenyl 2-acetamido-2-deoxy-β-D-galactopyranoside	570	0.64	890	pH 6, 25 °C	doi:10.1271/bbb1961.51.1679	Open record: integumental β-N-acetyl-D-glucosaminidase Ex1, p-nitrophenyl 2-acetamido-2-deoxy-β-D-galactopyranoside
integumental β-N-acetyl-D-glucosaminidase Ex1	Bombyx mori	p-nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside	890	0.49	1.81 × 10³	pH 6, 25 °C	doi:10.1271/bbb1961.51.1679	Open record: integumental β-N-acetyl-D-glucosaminidase Ex1, p-nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside
integumental β-N-acetyl-D-glucosaminidase Ex1	Bombyx mori	penta-N-acetylchitopentaose	57	0.07	860	pH 6, 25 °C	doi:10.1271/bbb1961.51.1679	Open record: integumental β-N-acetyl-D-glucosaminidase Ex1, penta-N-acetylchitopentaose
integumental β-N-acetyl-D-glucosaminidase Ex2	Bombyx mori	hexa-N-acetylchitohexaose	29	0.06	500	pH 6, 25 °C	doi:10.1271/bbb1961.51.1679	Open record: integumental β-N-acetyl-D-glucosaminidase Ex2, hexa-N-acetylchitohexaose
integumental β-N-acetyl-D-glucosaminidase Ex2	Bombyx mori	N,N',N'',N'''-tetraacetylchitotetraose	42	0.03	1.4 × 10³	pH 6, 25 °C	doi:10.1271/bbb1961.51.1679	Open record: integumental β-N-acetyl-D-glucosaminidase Ex2, N,N',N'',N'''-tetraacetylchitotetraose
integumental β-N-acetyl-D-glucosaminidase Ex2	Bombyx mori	N,N',N''-triacetylchitotriose	82	0.05	1.71 × 10³	pH 6, 25 °C	doi:10.1271/bbb1961.51.1679	Open record: integumental β-N-acetyl-D-glucosaminidase Ex2, N,N',N''-triacetylchitotriose
integumental β-N-acetyl-D-glucosaminidase Ex2	Bombyx mori	N,N'-diacetylchitobiose	580	0.64	910	pH 6, 25 °C	doi:10.1271/bbb1961.51.1679	Open record: integumental β-N-acetyl-D-glucosaminidase Ex2, N,N'-diacetylchitobiose
integumental β-N-acetyl-D-glucosaminidase Ex2	Bombyx mori	p-nitrophenyl 2-acetamido-2-deoxy-β-D-galactopyranoside	320	0.31	1.05 × 10³	pH 6, 25 °C	doi:10.1271/bbb1961.51.1679	Open record: integumental β-N-acetyl-D-glucosaminidase Ex2, p-nitrophenyl 2-acetamido-2-deoxy-β-D-galactopyranoside
integumental β-N-acetyl-D-glucosaminidase Ex2	Bombyx mori	p-nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside	720	0.42	1.7 × 10³	pH 6, 25 °C	doi:10.1271/bbb1961.51.1679	Open record: integumental β-N-acetyl-D-glucosaminidase Ex2, p-nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside
integumental β-N-acetyl-D-glucosaminidase Ex2	Bombyx mori	penta-N-acetylchitopentaose	76	0.09	830	pH 6, 25 °C	doi:10.1271/bbb1961.51.1679	Open record: integumental β-N-acetyl-D-glucosaminidase Ex2, penta-N-acetylchitopentaose
Nag3 (beta-N-acetylglucosaminidase/beta-glucosidase, family 3)	Cellulomonas fimi	4'-nitrophenyl beta-D-glucopyranoside	—	—	0.076	pH 7.08, 25 °C	PMID 16762038	Open record: Nag3 (beta-N-acetylglucosaminidase/beta-glucosidase, family 3), 4'-nitrophenyl beta-D-glucopyranoside
Nag3 (beta-N-acetylglucosaminidase/beta-glucosidase, family 3)	Cellulomonas fimi	4'-nitrophenyl beta-N-acetyl-D-glucosaminide	0.18	2.7	0.067	pH 7.08, 25 °C	PMID 16762038	Open record: Nag3 (beta-N-acetylglucosaminidase/beta-glucosidase, family 3), 4'-nitrophenyl beta-N-acetyl-D-glucosaminide
NagA (β-N-acetylglucosaminidase)	Thermotoga maritima	p-nitrophenyl di-N-acetyl β-D-chitobiose	199	0.37	538	pH 7.5, 70 °C	PMID 19914575	Open record: NagA (β-N-acetylglucosaminidase), p-nitrophenyl di-N-acetyl β-D-chitobiose
NagA (β-N-acetylglucosaminidase)	Thermotoga maritima	p-nitrophenyl N-acetyl β-D-glucosaminide	246.2	0.28	879.3	pH 7.5, 70 °C	PMID 19914575	Open record: NagA (β-N-acetylglucosaminidase), p-nitrophenyl N-acetyl β-D-glucosaminide
NagA (β-N-acetylglucosaminidase)	Thermotoga maritima	p-nitrophenyl tri-N-acetyl β-D-chitotriose	146.7	0.29	505.7	pH 7.5, 70 °C	PMID 19914575	Open record: NagA (β-N-acetylglucosaminidase), p-nitrophenyl tri-N-acetyl β-D-chitotriose
OfHex1 (beta-N-acetyl-D-hexosaminidase)	Ostrinia furnacalis	4-methylumbelliferyl N-acetyl-beta-D-glucosaminide	434.7	0.107	4063	25 °C	PMID 21106526	Open record: OfHex1 (beta-N-acetyl-D-hexosaminidase), 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
OfHex1 (beta-N-acetyl-D-hexosaminidase)	Ostrinia furnacalis	N,N'-diacetylchitobiose	507.4	0.148	3428	pH 7, 25 °C	PMID 21106526	Open record: OfHex1 (beta-N-acetyl-D-hexosaminidase), N,N'-diacetylchitobiose
OfHex1 (beta-N-acetyl-D-hexosaminidase)variant E328A	Ostrinia furnacalis	4-methylumbelliferyl N-acetyl-beta-D-glucosaminide	356	0.096	3708	25 °C	PMID 21106526	Open record: OfHex1 (beta-N-acetyl-D-hexosaminidase) variant E328A, 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
OfHex1 (beta-N-acetyl-D-hexosaminidase)variant E328Q	Ostrinia furnacalis	4-methylumbelliferyl N-acetyl-beta-D-glucosaminide	349	0.105	3324	25 °C	PMID 21106526	Open record: OfHex1 (beta-N-acetyl-D-hexosaminidase) variant E328Q, 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
OfHex1 (beta-N-acetyl-D-hexosaminidase)variant H433A	Ostrinia furnacalis	4-methylumbelliferyl N-acetyl-beta-D-glucosaminide	0.313	0.095	3.295	25 °C	PMID 21106526	Open record: OfHex1 (beta-N-acetyl-D-hexosaminidase) variant H433A, 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
OfHex1 (beta-N-acetyl-D-hexosaminidase)variant V327G	Ostrinia furnacalis	4-methylumbelliferyl N-acetyl-beta-D-glucosaminide	189	0.07	2.7 × 10³	25 °C	PMID 21106526	Open record: OfHex1 (beta-N-acetyl-D-hexosaminidase) variant V327G, 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
OfHex1 (beta-N-acetyl-D-hexosaminidase)variant V327G	Ostrinia furnacalis	N,N'-diacetylchitobiose	337	0.126	2675	pH 7, 25 °C	PMID 21106526	Open record: OfHex1 (beta-N-acetyl-D-hexosaminidase) variant V327G, N,N'-diacetylchitobiose
OfHex1 (beta-N-acetyl-D-hexosaminidase)variant W448A	Ostrinia furnacalis	4-methylumbelliferyl N-acetyl-beta-D-glucosaminide	0.469	0.223	2.103	25 °C	PMID 21106526	Open record: OfHex1 (beta-N-acetyl-D-hexosaminidase) variant W448A, 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
OfHex1 (beta-N-acetyl-D-hexosaminidase)variant W448F	Ostrinia furnacalis	4-methylumbelliferyl N-acetyl-beta-D-glucosaminide	0.567	0.149	3.805	25 °C	PMID 21106526	Open record: OfHex1 (beta-N-acetyl-D-hexosaminidase) variant W448F, 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
OfHex1 (beta-N-acetyl-D-hexosaminidase)variant W490A	Ostrinia furnacalis	4-methylumbelliferyl N-acetyl-beta-D-glucosaminide	167.6	0.1	1676	25 °C	PMID 21106526	Open record: OfHex1 (beta-N-acetyl-D-hexosaminidase) variant W490A, 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
OfHex1 (beta-N-acetyl-D-hexosaminidase)variant W490A	Ostrinia furnacalis	N,N'-diacetylchitobiose	449.6	1.875	239.8	pH 7, 25 °C	PMID 21106526	Open record: OfHex1 (beta-N-acetyl-D-hexosaminidase) variant W490A, N,N'-diacetylchitobiose
SSO3039 (bifunctional β-glucosidase/β-N-acetylglucosaminidase, GH116)	Sulfolobus solfataricus	4-methylumbelliferyl N-acetyl-β-D-glucosaminide	5.4	1.1	4.9	pH 4, 60 °C	PMID 24060745	Open record: SSO3039 (bifunctional β-glucosidase/β-N-acetylglucosaminidase, GH116), 4-methylumbelliferyl N-acetyl-β-D-glucosaminide
SSO3039 (bifunctional β-glucosidase/β-N-acetylglucosaminidase, GH116)	Sulfolobus solfataricus	4-nitrophenyl N-acetyl-β-D-glucosaminide	19.2	4.15	1.6	pH 4, 60 °C	PMID 24060745	Open record: SSO3039 (bifunctional β-glucosidase/β-N-acetylglucosaminidase, GH116), 4-nitrophenyl N-acetyl-β-D-glucosaminide
StrHvariant C469Y	Streptococcus pneumoniae	4-nitrophenyl N-acetyl-beta-D-glucosaminide	5.1	0.65	7.8	pH 7.5, 37 °C	PMID 22013074	Open record: StrH variant C469Y, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
StrHvariant E361A	Streptococcus pneumoniae	4-nitrophenyl N-acetyl-beta-D-glucosaminide	0.4	0.9	0.4	pH 7.5, 37 °C	PMID 22013074	Open record: StrH variant E361A, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
StrHvariant GH20-1	Streptococcus pneumoniae	4-nitrophenyl N-acetyl-beta-D-glucosaminide	1.3	0.85	1.5	pH 7.5, 37 °C	PMID 22013074	Open record: StrH variant GH20-1, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
StrHvariant GH20-1	Streptococcus pneumoniae	NAGbeta(1,2)Man	0.025	1.03	0.0243	pH 5, 37 °C	PMID 22013074	Open record: StrH variant GH20-1, NAGbeta(1,2)Man
StrHvariant GH20-1&2	Streptococcus pneumoniae	4-nitrophenyl N-acetyl-beta-D-glucosaminide	11	0.5	22	pH 7.5, 37 °C	PMID 22013074	Open record: StrH variant GH20-1&2, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
StrHvariant GH20-1&2	Streptococcus pneumoniae	NAGbeta(1,2)Man	0.4583	0.98	0.645	pH 5, 37 °C	PMID 22013074	Open record: StrH variant GH20-1&2, NAGbeta(1,2)Man
StrHvariant GH20-1&L	Streptococcus pneumoniae	4-nitrophenyl N-acetyl-beta-D-glucosaminide	1.4	0.7	2	pH 7.5, 37 °C	PMID 22013074	Open record: StrH variant GH20-1&L, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
StrHvariant GH20-2	Streptococcus pneumoniae	4-nitrophenyl N-acetyl-beta-D-glucosaminide	4.8	0.837	5.8	pH 7.5, 37 °C	PMID 22013074	Open record: StrH variant GH20-2, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
StrHvariant GH20-2	Streptococcus pneumoniae	NAGbeta(1,2)Man	0.1017	1.31	0.0777	pH 5, 37 °C	PMID 22013074	Open record: StrH variant GH20-2, NAGbeta(1,2)Man
StrHvariant GH20-2&L	Streptococcus pneumoniae	4-nitrophenyl N-acetyl-beta-D-glucosaminide	4.9	0.787	6.3	pH 7.5, 37 °C	PMID 22013074	Open record: StrH variant GH20-2&L, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
StrHvariant W443A	Streptococcus pneumoniae	4-nitrophenyl N-acetyl-beta-D-glucosaminide	0.015	0.857	0.018	pH 7.5, 37 °C	PMID 22013074	Open record: StrH variant W443A, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
StrHvariant W443A	Streptococcus pneumoniae	NAGbeta(1,2)Man	0.0087	1.34	0.0065	pH 5, 37 °C	PMID 22013074	Open record: StrH variant W443A, NAGbeta(1,2)Man
StrHvariant W443A/W876A	Streptococcus pneumoniae	4-nitrophenyl N-acetyl-beta-D-glucosaminide	0.04	0.9	0.044	pH 7.5, 37 °C	PMID 22013074	Open record: StrH variant W443A/W876A, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
StrHvariant W443A/W876A	Streptococcus pneumoniae	NAGbeta(1,2)Man	0.0097	1.3	0.0073	pH 5, 37 °C	PMID 22013074	Open record: StrH variant W443A/W876A, NAGbeta(1,2)Man
StrHvariant W443F	Streptococcus pneumoniae	4-nitrophenyl N-acetyl-beta-D-glucosaminide	0.11	1.12	0.09	pH 7.5, 37 °C	PMID 22013074	Open record: StrH variant W443F, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
StrHvariant W876A	Streptococcus pneumoniae	4-nitrophenyl N-acetyl-beta-D-glucosaminide	0.51	1	0.51	pH 7.5, 37 °C	PMID 22013074	Open record: StrH variant W876A, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
StrHvariant W876A	Streptococcus pneumoniae	NAGbeta(1,2)Man	0.009	1.51	0.006	pH 5, 37 °C	PMID 22013074	Open record: StrH variant W876A, NAGbeta(1,2)Man
StrHvariant W876F	Streptococcus pneumoniae	4-nitrophenyl N-acetyl-beta-D-glucosaminide	0.98	1.292	0.75	pH 7.5, 37 °C	PMID 22013074	Open record: StrH variant W876F, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
StrHvariant Y482A	Streptococcus pneumoniae	4-nitrophenyl N-acetyl-beta-D-glucosaminide	0.46	1.8	0.25	pH 7.5, 37 °C	PMID 22013074	Open record: StrH variant Y482A, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
StrHvariant Y482F	Streptococcus pneumoniae	4-nitrophenyl N-acetyl-beta-D-glucosaminide	0.66	1.5	0.44	pH 7.5, 37 °C	PMID 22013074	Open record: StrH variant Y482F, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
StrHvariant Y914A	Streptococcus pneumoniae	4-nitrophenyl N-acetyl-beta-D-glucosaminide	0.033	1.56	0.022	pH 7.5, 37 °C	PMID 22013074	Open record: StrH variant Y914A, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
StrHvariant Y914F	Streptococcus pneumoniae	4-nitrophenyl N-acetyl-beta-D-glucosaminide	4.7	0.95	5	pH 7.5, 37 °C	PMID 22013074	Open record: StrH variant Y914F, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
β-N-acetylhexosaminidase I (BbhI)variant D746E	Bifidobacterium bifidum	4-nitrophenyl N-acetyl-beta-D-galactosaminide	0.0021	6.8	3.1 × 10⁻⁴ calc.	pH 7.5, 37 °C	PMID 42200609	Open record: β-N-acetylhexosaminidase I (BbhI) variant D746E, 4-nitrophenyl N-acetyl-beta-D-galactosaminide
β-N-acetylhexosaminidase I (BbhI)variant D746E	Bifidobacterium bifidum	4-nitrophenyl N-acetyl-beta-D-glucosaminide1% DMSO; 10 mM lactose; Michaelis-Menten fit	1.03	0.78	1.3 calc.	pH 7.5, 37 °C	PMID 42200609	Open record: β-N-acetylhexosaminidase I (BbhI) variant D746E, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
β-N-acetylhexosaminidase I (BbhI)variant D746E	Bifidobacterium bifidum	4-nitrophenyl N-acetyl-beta-D-glucosaminide10% DMSO; 50 mM lactose; Michaelis-Menten fit	0.76	3.07	0.25 calc.	pH 7.5, 37 °C	PMID 42200609	Open record: β-N-acetylhexosaminidase I (BbhI) variant D746E, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
β-N-acetylhexosaminidase I (BbhI)variant D746E	Bifidobacterium bifidum	4-nitrophenyl N-acetyl-beta-D-glucosaminide0% DMSO; 10 mM lactose; Lineweaver-Burk fit	2.82	1.44	1.96 calc.	pH 7.5, 37 °C	PMID 42200609	Open record: β-N-acetylhexosaminidase I (BbhI) variant D746E, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
β-N-acetylhexosaminidase I (BbhI)variant D746E	Bifidobacterium bifidum	4-nitrophenyl N-acetyl-beta-D-glucosaminide1% DMSO; 10 mM lactose; Lineweaver-Burk fit	1.18	1.06	1.11 calc.	pH 7.5, 37 °C	PMID 42200609	Open record: β-N-acetylhexosaminidase I (BbhI) variant D746E, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
β-N-acetylhexosaminidase I (BbhI)variant D746E	Bifidobacterium bifidum	4-nitrophenyl N-acetyl-beta-D-glucosaminide10% DMSO; 50 mM lactose; Lineweaver-Burk fit	0.5	1.56	0.32 calc.	pH 7.5, 37 °C	PMID 42200609	Open record: β-N-acetylhexosaminidase I (BbhI) variant D746E, 4-nitrophenyl N-acetyl-beta-D-glucosaminide
β-N-acetylhexosaminidase I (BbhI)variant D746E	Bifidobacterium bifidum	4-nitrophenyl N-acetyl-β-D-galactosaminide	0.0019	5.8	3.3 × 10⁻⁴	pH 7.5, 37 °C	PMID 42200609	Open record: β-N-acetylhexosaminidase I (BbhI) variant D746E, 4-nitrophenyl N-acetyl-β-D-galactosaminide
β-N-acetylhexosaminidase I (BbhI)variant D746E	Bifidobacterium bifidum	4-nitrophenyl N-acetyl-β-D-glucosaminide10 mM lactose (acceptor); no DMSO	2.01	0.77	2.6	pH 7.5, 37 °C	PMID 42200609	Open record: β-N-acetylhexosaminidase I (BbhI) variant D746E, 4-nitrophenyl N-acetyl-β-D-glucosaminide
β-N-acetylhexosaminidase I (BbhI)variant D746E	Bifidobacterium bifidum	4-nitrophenyl N-acetyl-β-D-glucosaminide50 mM lactose (acceptor); 10% DMSO	—	—	0.32	pH 7.5, 37 °C	PMID 42200609	Open record: β-N-acetylhexosaminidase I (BbhI) variant D746E, 4-nitrophenyl N-acetyl-β-D-glucosaminide
β-N-acetylhexosaminidase I (BbhI)variant D746E	Bifidobacterium bifidum	N-acetylgalactosamine 1,2-oxazoline	56	25.3	2.2	pH 7.5, 37 °C	PMID 42200609	Open record: β-N-acetylhexosaminidase I (BbhI) variant D746E, N-acetylgalactosamine 1,2-oxazoline
β-N-acetylhexosaminidase I (BbhI)variant D746E	Bifidobacterium bifidum	N-acetylglucosamine 1,2-oxazoline	277	8.3	33.4	pH 7.5, 37 °C	PMID 42200609	Open record: β-N-acetylhexosaminidase I (BbhI) variant D746E, N-acetylglucosamine 1,2-oxazoline
β-N-acetylhexosaminidase I (BbhI)variant D746E	Bifidobacterium bifidum	N-acetylmannosamine 1,2-oxazoline	10.7	36.6	0.29	pH 7.5, 37 °C	PMID 42200609	Open record: β-N-acetylhexosaminidase I (BbhI) variant D746E, N-acetylmannosamine 1,2-oxazoline