Values CatLog read from this paper
Catalytic Efficiency for the Chemoenzymatic Synthesis of Core Human Milk Oligosaccharides and Analogs via Sugar Oxazolines
PMID 42200609doi:10.1021/acs.jafc.5c12942Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (28 values) from this paper: β-N-acetylhexosaminidase I (BbhI) from Bifidobacterium bifidum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| β-N-acetylhexosaminidase I (BbhI)variant D746E | Bifidobacterium bifidum | 4-nitrophenyl N-acetyl-beta-D-galactosaminide | 0.0021 | 6.8 | 3.1 × 10⁻⁴ calc. | pH 7.5, 37 °C | Open record: β-N-acetylhexosaminidase I (BbhI) variant D746E, 4-nitrophenyl N-acetyl-beta-D-galactosaminide |
| β-N-acetylhexosaminidase I (BbhI)variant D746E | Bifidobacterium bifidum | 4-nitrophenyl N-acetyl-beta-D-glucosaminide1% DMSO; 10 mM lactose; Michaelis-Menten fit | 1.03 | 0.78 | 1.3 calc. | pH 7.5, 37 °C | Open record: β-N-acetylhexosaminidase I (BbhI) variant D746E, 4-nitrophenyl N-acetyl-beta-D-glucosaminide |
| β-N-acetylhexosaminidase I (BbhI)variant D746E | Bifidobacterium bifidum | 4-nitrophenyl N-acetyl-beta-D-glucosaminide10% DMSO; 50 mM lactose; Michaelis-Menten fit | 0.76 | 3.07 | 0.25 calc. | pH 7.5, 37 °C | Open record: β-N-acetylhexosaminidase I (BbhI) variant D746E, 4-nitrophenyl N-acetyl-beta-D-glucosaminide |
| β-N-acetylhexosaminidase I (BbhI)variant D746E | Bifidobacterium bifidum | 4-nitrophenyl N-acetyl-beta-D-glucosaminide0% DMSO; 10 mM lactose; Lineweaver-Burk fit | 2.82 | 1.44 | 1.96 calc. | pH 7.5, 37 °C | Open record: β-N-acetylhexosaminidase I (BbhI) variant D746E, 4-nitrophenyl N-acetyl-beta-D-glucosaminide |
| β-N-acetylhexosaminidase I (BbhI)variant D746E | Bifidobacterium bifidum | 4-nitrophenyl N-acetyl-beta-D-glucosaminide1% DMSO; 10 mM lactose; Lineweaver-Burk fit | 1.18 | 1.06 | 1.11 calc. | pH 7.5, 37 °C | Open record: β-N-acetylhexosaminidase I (BbhI) variant D746E, 4-nitrophenyl N-acetyl-beta-D-glucosaminide |
| β-N-acetylhexosaminidase I (BbhI)variant D746E | Bifidobacterium bifidum | 4-nitrophenyl N-acetyl-beta-D-glucosaminide10% DMSO; 50 mM lactose; Lineweaver-Burk fit | 0.5 | 1.56 | 0.32 calc. | pH 7.5, 37 °C | Open record: β-N-acetylhexosaminidase I (BbhI) variant D746E, 4-nitrophenyl N-acetyl-beta-D-glucosaminide |
| β-N-acetylhexosaminidase I (BbhI)variant D746E | Bifidobacterium bifidum | 4-nitrophenyl N-acetyl-β-D-galactosaminide | 0.0019 | 5.8 | 3.3 × 10⁻⁴ | pH 7.5, 37 °C | Open record: β-N-acetylhexosaminidase I (BbhI) variant D746E, 4-nitrophenyl N-acetyl-β-D-galactosaminide |
| β-N-acetylhexosaminidase I (BbhI)variant D746E | Bifidobacterium bifidum | 4-nitrophenyl N-acetyl-β-D-glucosaminide10 mM lactose (acceptor); no DMSO | 2.01 | 0.77 | 2.6 | pH 7.5, 37 °C | Open record: β-N-acetylhexosaminidase I (BbhI) variant D746E, 4-nitrophenyl N-acetyl-β-D-glucosaminide |
| β-N-acetylhexosaminidase I (BbhI)variant D746E | Bifidobacterium bifidum | 4-nitrophenyl N-acetyl-β-D-glucosaminide50 mM lactose (acceptor); 10% DMSO | — | — | 0.32 | pH 7.5, 37 °C | Open record: β-N-acetylhexosaminidase I (BbhI) variant D746E, 4-nitrophenyl N-acetyl-β-D-glucosaminide |
| β-N-acetylhexosaminidase I (BbhI)variant D746E | Bifidobacterium bifidum | N-acetylgalactosamine 1,2-oxazoline | 56 | 25.3 | 2.2 | pH 7.5, 37 °C | Open record: β-N-acetylhexosaminidase I (BbhI) variant D746E, N-acetylgalactosamine 1,2-oxazoline |
| β-N-acetylhexosaminidase I (BbhI)variant D746E | Bifidobacterium bifidum | N-acetylglucosamine 1,2-oxazoline | 277 | 8.3 | 33.4 | pH 7.5, 37 °C | Open record: β-N-acetylhexosaminidase I (BbhI) variant D746E, N-acetylglucosamine 1,2-oxazoline |
| β-N-acetylhexosaminidase I (BbhI)variant D746E | Bifidobacterium bifidum | N-acetylmannosamine 1,2-oxazoline | 10.7 | 36.6 | 0.29 | pH 7.5, 37 °C | Open record: β-N-acetylhexosaminidase I (BbhI) variant D746E, N-acetylmannosamine 1,2-oxazoline |