Values CatLog read from this paper

Sequence and Structural Motifs Controlling the Broad Substrate Specificity of the Mycobacterial Hormone-Sensitive Lipase LipN

PMID 37065048doi:10.1021/acsomega.3c00534Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 93 records (271 values) from this paper: MmLipN, LipN (MmLipN) from Mycobacterium marinum.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.1.1.79

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
LipN (MmLipN)	Mycobacterium marinum	fluorogenic ester substrate 1 (acetyl ester)	—	—	460	pH 7.4	Open record: LipN (MmLipN), fluorogenic ester substrate 1 (acetyl ester)
LipN (MmLipN)	Mycobacterium marinum	fluorogenic ester substrate 20 (oxazole ester)	—	—	91	pH 7.4	Open record: LipN (MmLipN), fluorogenic ester substrate 20 (oxazole ester)
LipN (MmLipN)	Mycobacterium marinum	fluorogenic ester substrate 27 (tertiary ester)	—	—	7.4	pH 7.4	Open record: LipN (MmLipN), fluorogenic ester substrate 27 (tertiary ester)
LipN (MmLipN)	Mycobacterium marinum	fluorogenic ester substrate 29 (tertiary ester)	—	—	2.6	pH 7.4	Open record: LipN (MmLipN), fluorogenic ester substrate 29 (tertiary ester)
MmLipN	Mycobacterium marinum	Fluorescein bis((((1-(2-pyridinyl))cyclopropyl)carboxymethyl ether)	2.6 × 10⁻⁵	4.9 × 10⁻⁵	0.53	pH 7.4	Open record: MmLipN, Fluorescein bis((((1-(2-pyridinyl))cyclopropyl)carboxymethyl ether)
MmLipN	Mycobacterium marinum	Fluorescein bis(((1-cyano)cyclopropyl)carboxymethyl ether)	0.0012	0.0017	0.67	pH 7.4	Open record: MmLipN, Fluorescein bis(((1-cyano)cyclopropyl)carboxymethyl ether)
MmLipN	Mycobacterium marinum	Fluorescein bis(((1-hydroxy)cyclopropyl)carboxymethyl ether))	0.0029	4 × 10⁻⁴	7.4	pH 7.4	Open record: MmLipN, Fluorescein bis(((1-hydroxy)cyclopropyl)carboxymethyl ether))
MmLipN	Mycobacterium marinum	Fluorescein bis(((1-methanesulfonyl)cyclopropyl)carboxymethyl ether)	1.4 × 10⁻⁵	8.4 × 10⁻⁵	0.17	pH 7.4	Open record: MmLipN, Fluorescein bis(((1-methanesulfonyl)cyclopropyl)carboxymethyl ether)
MmLipN	Mycobacterium marinum	Fluorescein bis(((1-phenyl)cyclopropyl)carboxymethyl ether)	5.8 × 10⁻⁵	0.013	0.0044	pH 7.4	Open record: MmLipN, Fluorescein bis(((1-phenyl)cyclopropyl)carboxymethyl ether)
MmLipN	Mycobacterium marinum	Fluorescein bis(((1-trifluoromethyl)cyclopropyl)carboxymethyl ether)	3.7 × 10⁻⁵	3.7 × 10⁻⁴	0.098	pH 7.4	Open record: MmLipN, Fluorescein bis(((1-trifluoromethyl)cyclopropyl)carboxymethyl ether)
MmLipN	Mycobacterium marinum	Fluorescein bis(((2,2-dichloro-1-methyl)cyclopropyl)carboxymethyl ether)	1.1 × 10⁻⁵	8.7 × 10⁻⁴	0.012	pH 7.4	Open record: MmLipN, Fluorescein bis(((2,2-dichloro-1-methyl)cyclopropyl)carboxymethyl ether)
MmLipN	Mycobacterium marinum	Fluorescein bis(((2,2-dimethyl)cyclopropyl)carboxymethyl ether)	0.0011	0.0017	0.66	pH 7.4	Open record: MmLipN, Fluorescein bis(((2,2-dimethyl)cyclopropyl)carboxymethyl ether)
MmLipN	Mycobacterium marinum	Fluorescein bis((2-methyl)propionyloxymethyl ether)	0.017	0.0095	1.8	pH 7.4	Open record: MmLipN, Fluorescein bis((2-methyl)propionyloxymethyl ether)
MmLipN	Mycobacterium marinum	Fluorescein bis((3-methyl)propargyloxymethyl ether)	0.0061	0.0018	3.3	pH 7.4	Open record: MmLipN, Fluorescein bis((3-methyl)propargyloxymethyl ether)
MmLipN	Mycobacterium marinum	Fluorescein bis((5-oxazolyl)carboxymethyl ether)	0.003	3.3 × 10⁻⁵	91	pH 7.4	Open record: MmLipN, Fluorescein bis((5-oxazolyl)carboxymethyl ether)
MmLipN	Mycobacterium marinum	Fluorescein bis(3-(2-methoxyethoxy)propionyloxymethyl ether)	5 × 10⁻⁴	0.021	0.026	pH 7.4	Open record: MmLipN, Fluorescein bis(3-(2-methoxyethoxy)propionyloxymethyl ether)
MmLipN	Mycobacterium marinum	Fluorescein bis(crotonyloxymethyl ether)	0.0029	5.1 × 10⁻⁵	5.7	pH 7.4	Open record: MmLipN, Fluorescein bis(crotonyloxymethyl ether)
MmLipN	Mycobacterium marinum	Fluorescein bis(cyanoacetyloxymethyl ether)	0.015	0.0031	4.9	pH 7.4	Open record: MmLipN, Fluorescein bis(cyanoacetyloxymethyl ether)
MmLipN	Mycobacterium marinum	Fluorescein bis(cyclopropyl)carboxymethyl ether)	9 × 10⁻⁴	3.7 × 10⁻⁴	2.6	pH 7.4	Open record: MmLipN, Fluorescein bis(cyclopropyl)carboxymethyl ether)
MmLipN	Mycobacterium marinum	fluorogenic ester substrate 1 (alkyl ester; acetyl ester)	0.1	2.2 × 10⁻⁴	460	pH 7.4	Open record: MmLipN, fluorogenic ester substrate 1 (alkyl ester; acetyl ester)
MmLipN	Mycobacterium marinum	fluorogenic ester substrate 12 (a/b unsaturated ester)	0.02	0.0034	6.1	pH 7.4	Open record: MmLipN, fluorogenic ester substrate 12 (a/b unsaturated ester)
MmLipN	Mycobacterium marinum	fluorogenic ester substrate 13 (a/b unsaturated ester)	0.0093	3.2 × 10⁻⁵	29	pH 7.4	Open record: MmLipN, fluorogenic ester substrate 13 (a/b unsaturated ester)
MmLipN	Mycobacterium marinum	fluorogenic ester substrate 16 (cycloalkyl ester)	0.025	0.003	8.3	pH 7.4	Open record: MmLipN, fluorogenic ester substrate 16 (cycloalkyl ester)
MmLipN	Mycobacterium marinum	fluorogenic ester substrate 17 (cycloalkyl ester; cyclopentyl ester)	0.0012	0.0339	0.029	pH 7.4	Open record: MmLipN, fluorogenic ester substrate 17 (cycloalkyl ester; cyclopentyl ester)
MmLipN	Mycobacterium marinum	fluorogenic ester substrate 18 (cycloalkyl ester)	7 × 10⁻⁴	0.029	0.025	pH 7.4	Open record: MmLipN, fluorogenic ester substrate 18 (cycloalkyl ester)
MmLipN	Mycobacterium marinum	fluorogenic ester substrate 19 (cycloalkyl ester; furan ester)	0.003	8.4 × 10⁻⁴	3.5	pH 7.4	Open record: MmLipN, fluorogenic ester substrate 19 (cycloalkyl ester; furan ester)
MmLipN	Mycobacterium marinum	fluorogenic ester substrate 2 (alkyl ester; propionyl ester)	0.18	0.0042	42	pH 7.4	Open record: MmLipN, fluorogenic ester substrate 2 (alkyl ester; propionyl ester)
MmLipN	Mycobacterium marinum	fluorogenic ester substrate 21 (cycloalkyl ester)	3 × 10⁻⁴	0.0012	0.31	pH 7.4	Open record: MmLipN, fluorogenic ester substrate 21 (cycloalkyl ester)
MmLipN	Mycobacterium marinum	fluorogenic ester substrate 22 (tertiary ester; pivalic tertiary ester)	2.4 × 10⁻⁵	7.1 × 10⁻⁴	0.034	pH 7.4	Open record: MmLipN, fluorogenic ester substrate 22 (tertiary ester; pivalic tertiary ester)
MmLipN	Mycobacterium marinum	fluorogenic ester substrate 3 (alkyl ester; butyl ester)	0.062	0.0062	10	pH 7.4	Open record: MmLipN, fluorogenic ester substrate 3 (alkyl ester; butyl ester)
MmLipN	Mycobacterium marinum	fluorogenic ester substrate 31 (fluorinated ester)	0.013	0.0011	11	pH 7.4	Open record: MmLipN, fluorogenic ester substrate 31 (fluorinated ester)
MmLipN	Mycobacterium marinum	fluorogenic ester substrate 32 (fluorinated ester)	2.3 × 10⁻⁴	0.047	0.0048	pH 7.4	Open record: MmLipN, fluorogenic ester substrate 32 (fluorinated ester)
MmLipN	Mycobacterium marinum	fluorogenic ester substrate 4 (alkyl ester)	0.024	0.0038	6.4	pH 7.4	Open record: MmLipN, fluorogenic ester substrate 4 (alkyl ester)
MmLipN	Mycobacterium marinum	fluorogenic ester substrate 5 (alkyl ester)	0.0074	0.0044	1.7	pH 7.4	Open record: MmLipN, fluorogenic ester substrate 5 (alkyl ester)
MmLipN	Mycobacterium marinum	fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)	0.11	0.0026	40	pH 7.4	Open record: MmLipN, fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)
MmLipN	Mycobacterium marinum	fluorogenic ester substrate 8 (polar ester)	0.093	0.0028	34	pH 7.4	Open record: MmLipN, fluorogenic ester substrate 8 (polar ester)
MmLipN	Mycobacterium marinum	fluorogenic ester substrate 9 (polar ester)	0.12	0.003	39	pH 7.4	Open record: MmLipN, fluorogenic ester substrate 9 (polar ester)
MmLipN	Mycobacterium marinum	p-nitrophenyl acetate	94	2.4	39	pH 7.4, 25 °C	Open record: MmLipN, p-nitrophenyl acetate
MmLipN	Mycobacterium marinum	p-nitrophenyl butyrate	190	0.02	9.3 × 10³	pH 7.4, 25 °C	Open record: MmLipN, p-nitrophenyl butyrate
MmLipN	Mycobacterium marinum	p-nitrophenyl myristate	0.28	0.12	2.3	pH 7.4, 25 °C	Open record: MmLipN, p-nitrophenyl myristate
MmLipN	Mycobacterium marinum	p-nitrophenyl octanoate	1.1	0.41	2.7	pH 7.4, 25 °C	Open record: MmLipN, p-nitrophenyl octanoate
MmLipN	Mycobacterium marinum	p-nitrophenyl valerate	15	0.25	59	pH 7.4, 25 °C	Open record: MmLipN, p-nitrophenyl valerate
MmLipNvariant D215A	Mycobacterium marinum	Fluorescein bis((5-oxazolyl)carboxymethyl ether)	0.0023	4.9 × 10⁻⁴	4.6	pH 7.4	Open record: MmLipN variant D215A, Fluorescein bis((5-oxazolyl)carboxymethyl ether)
MmLipNvariant D215A	Mycobacterium marinum	fluorogenic ester substrate 1 (alkyl ester; acetyl ester)	0.038	0.0025	16	pH 7.4	Open record: MmLipN variant D215A, fluorogenic ester substrate 1 (alkyl ester; acetyl ester)
MmLipNvariant D215A	Mycobacterium marinum	fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)	0.039	0.0033	12	pH 7.4	Open record: MmLipN variant D215A, fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)
MmLipNvariant D269A	Mycobacterium marinum	Fluorescein bis((5-oxazolyl)carboxymethyl ether)	0.046	0.0057	1.7	pH 7.4	Open record: MmLipN variant D269A, Fluorescein bis((5-oxazolyl)carboxymethyl ether)
MmLipNvariant D269A	Mycobacterium marinum	fluorogenic ester substrate 1 (alkyl ester; acetyl ester)	0.0055	0.0058	0.96	pH 7.4	Open record: MmLipN variant D269A, fluorogenic ester substrate 1 (alkyl ester; acetyl ester)
MmLipNvariant D269A	Mycobacterium marinum	fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)	0.032	0.015	2.2	pH 7.4	Open record: MmLipN variant D269A, fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)
MmLipNvariant D312A	Mycobacterium marinum	Fluorescein bis((5-oxazolyl)carboxymethyl ether)	0.0039	0.0057	4.3	pH 7.4	Open record: MmLipN variant D312A, Fluorescein bis((5-oxazolyl)carboxymethyl ether)
MmLipNvariant D312A	Mycobacterium marinum	fluorogenic ester substrate 1 (alkyl ester; acetyl ester)	0.19	0.028	6.7	pH 7.4	Open record: MmLipN variant D312A, fluorogenic ester substrate 1 (alkyl ester; acetyl ester)
MmLipNvariant D312A	Mycobacterium marinum	fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)	0.31	0.035	9	pH 7.4	Open record: MmLipN variant D312A, fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)
MmLipNvariant G144A	Mycobacterium marinum	Fluorescein bis((5-oxazolyl)carboxymethyl ether)	0.0029	0.0015	2	pH 7.4	Open record: MmLipN variant G144A, Fluorescein bis((5-oxazolyl)carboxymethyl ether)
MmLipNvariant G144A	Mycobacterium marinum	fluorogenic ester substrate 1 (alkyl ester; acetyl ester)	0.017	0.0054	3.3	pH 7.4	Open record: MmLipN variant G144A, fluorogenic ester substrate 1 (alkyl ester; acetyl ester)
MmLipNvariant G144A	Mycobacterium marinum	fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)	0.066	0.06	1.1	pH 7.4	Open record: MmLipN variant G144A, fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)
MmLipNvariant G145A	Mycobacterium marinum	Fluorescein bis((5-oxazolyl)carboxymethyl ether)	0.0019	3 × 10⁻⁴	6.2	pH 7.4	Open record: MmLipN variant G145A, Fluorescein bis((5-oxazolyl)carboxymethyl ether)
MmLipNvariant G145A	Mycobacterium marinum	fluorogenic ester substrate 1 (alkyl ester; acetyl ester)	0.045	0.0034	13	pH 7.4	Open record: MmLipN variant G145A, fluorogenic ester substrate 1 (alkyl ester; acetyl ester)
MmLipNvariant G145A	Mycobacterium marinum	fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)	0.18	0.12	1.5	pH 7.4	Open record: MmLipN variant G145A, fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)
MmLipNvariant H154A	Mycobacterium marinum	Fluorescein bis((5-oxazolyl)carboxymethyl ether)	0.0024	0.0034	0.7	pH 7.4	Open record: MmLipN variant H154A, Fluorescein bis((5-oxazolyl)carboxymethyl ether)
MmLipNvariant H154A	Mycobacterium marinum	fluorogenic ester substrate 1 (alkyl ester; acetyl ester)	0.03	0.032	0.94	pH 7.4	Open record: MmLipN variant H154A, fluorogenic ester substrate 1 (alkyl ester; acetyl ester)
MmLipNvariant H154A	Mycobacterium marinum	fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)	0.28	0.694	0.41	pH 7.4	Open record: MmLipN variant H154A, fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)
MmLipNvariant H342A	Mycobacterium marinum	Fluorescein bis((5-oxazolyl)carboxymethyl ether)	3.3 × 10⁻⁶	3.1 × 10⁻⁴	0.011	pH 7.4	Open record: MmLipN variant H342A, Fluorescein bis((5-oxazolyl)carboxymethyl ether)
MmLipNvariant H342A	Mycobacterium marinum	fluorogenic ester substrate 1 (alkyl ester; acetyl ester)	3.2 × 10⁻⁶	9.2 × 10⁻⁴	0.0035	pH 7.4	Open record: MmLipN variant H342A, fluorogenic ester substrate 1 (alkyl ester; acetyl ester)
MmLipNvariant H342A	Mycobacterium marinum	fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)	5.3 × 10⁻⁵	0.02	0.0026	pH 7.4	Open record: MmLipN variant H342A, fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)
MmLipNvariant R246A	Mycobacterium marinum	Fluorescein bis((5-oxazolyl)carboxymethyl ether)	0.089	0.12	0.76	pH 7.4	Open record: MmLipN variant R246A, Fluorescein bis((5-oxazolyl)carboxymethyl ether)
MmLipNvariant R246A	Mycobacterium marinum	fluorogenic ester substrate 1 (alkyl ester; acetyl ester)	0.054	0.021	2.5	pH 7.4	Open record: MmLipN variant R246A, fluorogenic ester substrate 1 (alkyl ester; acetyl ester)
MmLipNvariant R246A	Mycobacterium marinum	fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)	0.1	0.15	0.68	pH 7.4	Open record: MmLipN variant R246A, fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)
MmLipNvariant S177A	Mycobacterium marinum	Fluorescein bis((5-oxazolyl)carboxymethyl ether)	0.019	0.002	9.4	pH 7.4	Open record: MmLipN variant S177A, Fluorescein bis((5-oxazolyl)carboxymethyl ether)
MmLipNvariant S177A	Mycobacterium marinum	fluorogenic ester substrate 1 (alkyl ester; acetyl ester)	1.3	0.0011	110	pH 7.4	Open record: MmLipN variant S177A, fluorogenic ester substrate 1 (alkyl ester; acetyl ester)
MmLipNvariant S177A	Mycobacterium marinum	fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)	1.8	0.0064	270	pH 7.4	Open record: MmLipN variant S177A, fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)
MmLipNvariant S216C	Mycobacterium marinum	Fluorescein bis((5-oxazolyl)carboxymethyl ether)	6.1 × 10⁻⁵	0.12	5.3 × 10⁻⁴	pH 7.4	Open record: MmLipN variant S216C, Fluorescein bis((5-oxazolyl)carboxymethyl ether)
MmLipNvariant S216C	Mycobacterium marinum	fluorogenic ester substrate 1 (alkyl ester; acetyl ester)	0.0031	0.082	0.037	pH 7.4	Open record: MmLipN variant S216C, fluorogenic ester substrate 1 (alkyl ester; acetyl ester)
MmLipNvariant S216C	Mycobacterium marinum	fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)	0.0032	0.06	0.053	pH 7.4	Open record: MmLipN variant S216C, fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)
MmLipNvariant S216G	Mycobacterium marinum	Fluorescein bis((5-oxazolyl)carboxymethyl ether)	2.1 × 10⁻⁵	0.0036	0.0057	pH 7.4	Open record: MmLipN variant S216G, Fluorescein bis((5-oxazolyl)carboxymethyl ether)
MmLipNvariant S216G	Mycobacterium marinum	fluorogenic ester substrate 1 (alkyl ester; acetyl ester)	7 × 10⁻⁴	0.021	0.034	pH 7.4	Open record: MmLipN variant S216G, fluorogenic ester substrate 1 (alkyl ester; acetyl ester)
MmLipNvariant S216G	Mycobacterium marinum	fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)	1.8 × 10⁻⁴	0.022	0.082	pH 7.4	Open record: MmLipN variant S216G, fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)
MmLipNvariant S216N	Mycobacterium marinum	Fluorescein bis((5-oxazolyl)carboxymethyl ether)	1.2 × 10⁻⁴	0.026	0.015	pH 7.4	Open record: MmLipN variant S216N, Fluorescein bis((5-oxazolyl)carboxymethyl ether)
MmLipNvariant S216N	Mycobacterium marinum	fluorogenic ester substrate 1 (alkyl ester; acetyl ester)	8.2 × 10⁻⁴	0.026	0.031	pH 7.4	Open record: MmLipN variant S216N, fluorogenic ester substrate 1 (alkyl ester; acetyl ester)
MmLipNvariant S216N	Mycobacterium marinum	fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)	0.0021	0.041	0.051	pH 7.4	Open record: MmLipN variant S216N, fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)
MmLipNvariant S226A	Mycobacterium marinum	Fluorescein bis((5-oxazolyl)carboxymethyl ether)	0.017	0.0011	15	pH 7.4	Open record: MmLipN variant S226A, Fluorescein bis((5-oxazolyl)carboxymethyl ether)
MmLipNvariant S226A	Mycobacterium marinum	fluorogenic ester substrate 1 (alkyl ester; acetyl ester)	3.6	0.02	180	pH 7.4	Open record: MmLipN variant S226A, fluorogenic ester substrate 1 (alkyl ester; acetyl ester)
MmLipNvariant S226A	Mycobacterium marinum	fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)	2.3	0.031	73	pH 7.4	Open record: MmLipN variant S226A, fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)
MmLipNvariant W146A	Mycobacterium marinum	Fluorescein bis((5-oxazolyl)carboxymethyl ether)	0.0013	7.1 × 10⁻⁴	1.8	pH 7.4	Open record: MmLipN variant W146A, Fluorescein bis((5-oxazolyl)carboxymethyl ether)
MmLipNvariant W146A	Mycobacterium marinum	fluorogenic ester substrate 1 (alkyl ester; acetyl ester)	0.075	0.002	37	pH 7.4	Open record: MmLipN variant W146A, fluorogenic ester substrate 1 (alkyl ester; acetyl ester)
MmLipNvariant W146A	Mycobacterium marinum	fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)	0.067	0.0011	62	pH 7.4	Open record: MmLipN variant W146A, fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)
MmLipNvariant W146C	Mycobacterium marinum	Fluorescein bis((5-oxazolyl)carboxymethyl ether)	6.1 × 10⁻⁵	0.12	5.3 × 10⁻⁴	pH 7.4	Open record: MmLipN variant W146C, Fluorescein bis((5-oxazolyl)carboxymethyl ether)
MmLipNvariant W146C	Mycobacterium marinum	fluorogenic ester substrate 1 (alkyl ester; acetyl ester)	0.013	0.023	0.57	pH 7.4	Open record: MmLipN variant W146C, fluorogenic ester substrate 1 (alkyl ester; acetyl ester)
MmLipNvariant W146C	Mycobacterium marinum	fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)	0.15	0.013	11	pH 7.4	Open record: MmLipN variant W146C, fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)
MmLipNvariant W146Q	Mycobacterium marinum	Fluorescein bis((5-oxazolyl)carboxymethyl ether)	0.002	0.0035	0.55	pH 7.4	Open record: MmLipN variant W146Q, Fluorescein bis((5-oxazolyl)carboxymethyl ether)
MmLipNvariant W146Q	Mycobacterium marinum	fluorogenic ester substrate 1 (alkyl ester; acetyl ester)	0.064	0.0014	45	pH 7.4	Open record: MmLipN variant W146Q, fluorogenic ester substrate 1 (alkyl ester; acetyl ester)
MmLipNvariant W146Q	Mycobacterium marinum	fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)	0.075	0.0022	34	pH 7.4	Open record: MmLipN variant W146Q, fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)
MmLipNvariant W146S	Mycobacterium marinum	Fluorescein bis((5-oxazolyl)carboxymethyl ether)	0.0017	0.0035	0.5	pH 7.4	Open record: MmLipN variant W146S, Fluorescein bis((5-oxazolyl)carboxymethyl ether)
MmLipNvariant W146S	Mycobacterium marinum	fluorogenic ester substrate 1 (alkyl ester; acetyl ester)	0.091	0.023	4	pH 7.4	Open record: MmLipN variant W146S, fluorogenic ester substrate 1 (alkyl ester; acetyl ester)
MmLipNvariant W146S	Mycobacterium marinum	fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)	0.17	0.0041	42	pH 7.4	Open record: MmLipN variant W146S, fluorogenic ester substrate 7 (polar ester; four-atom chain polar/alkyl ether ester)