Values CatLog read from this paper

Enhancing the activity and succinyl-CoA specificity of 3-ketoacyl-CoA thiolase Tfu_0875 through rational binding pocket engineering

PMID 38694995doi:10.1016/j.synbio.2024.04.014Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 16 records (48 values) from this paper: 3-ketoacyl-CoA thiolase Tfu_0875 from Thermobifida fusca.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.3.1.9

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
3-ketoacyl-CoA thiolase Tfu_0875	Thermobifida fusca	acetyl-CoA	0.0035	2.28	0.0015	pH 7.4, 37 °C	Open record: 3-ketoacyl-CoA thiolase Tfu_0875, acetyl-CoA
3-ketoacyl-CoA thiolase Tfu_0875	Thermobifida fusca	glutaryl-CoA	0.0035	2.33	0.0015	pH 7.4, 37 °C	Open record: 3-ketoacyl-CoA thiolase Tfu_0875, glutaryl-CoA
3-ketoacyl-CoA thiolase Tfu_0875	Thermobifida fusca	malonyl-CoA	0.0032	3.32	0.001	pH 7.4, 37 °C	Open record: 3-ketoacyl-CoA thiolase Tfu_0875, malonyl-CoA
3-ketoacyl-CoA thiolase Tfu_0875	Thermobifida fusca	succinyl-CoA	0.0053	2.1	0.0025	pH 7.4, 37 °C	Open record: 3-ketoacyl-CoA thiolase Tfu_0875, succinyl-CoA
3-ketoacyl-CoA thiolase Tfu_0875variant N249W	Thermobifida fusca	acetyl-CoA	0.0052	1.15	0.0045	pH 7.4, 37 °C	Open record: 3-ketoacyl-CoA thiolase Tfu_0875 variant N249W, acetyl-CoA
3-ketoacyl-CoA thiolase Tfu_0875variant N249W	Thermobifida fusca	glutaryl-CoA	0.0033	2.27	0.0015	pH 7.4, 37 °C	Open record: 3-ketoacyl-CoA thiolase Tfu_0875 variant N249W, glutaryl-CoA
3-ketoacyl-CoA thiolase Tfu_0875variant N249W	Thermobifida fusca	malonyl-CoA	0.0035	2.89	0.0012	pH 7.4, 37 °C	Open record: 3-ketoacyl-CoA thiolase Tfu_0875 variant N249W, malonyl-CoA
3-ketoacyl-CoA thiolase Tfu_0875variant N249W	Thermobifida fusca	succinyl-CoA	0.0055	1.37	0.004	pH 7.4, 37 °C	Open record: 3-ketoacyl-CoA thiolase Tfu_0875 variant N249W, succinyl-CoA
3-ketoacyl-CoA thiolase Tfu_0875variant N249W/L163H	Thermobifida fusca	acetyl-CoA	0.0037	2.19	0.0017	pH 7.4, 37 °C	Open record: 3-ketoacyl-CoA thiolase Tfu_0875 variant N249W/L163H, acetyl-CoA
3-ketoacyl-CoA thiolase Tfu_0875variant N249W/L163H	Thermobifida fusca	glutaryl-CoA	0.0033	2.28	0.0015	pH 7.4, 37 °C	Open record: 3-ketoacyl-CoA thiolase Tfu_0875 variant N249W/L163H, glutaryl-CoA
3-ketoacyl-CoA thiolase Tfu_0875variant N249W/L163H	Thermobifida fusca	malonyl-CoA	0.0033	2.77	0.0012	pH 7.4, 37 °C	Open record: 3-ketoacyl-CoA thiolase Tfu_0875 variant N249W/L163H, malonyl-CoA
3-ketoacyl-CoA thiolase Tfu_0875variant N249W/L163H	Thermobifida fusca	succinyl-CoA	0.0065	1.14	0.0057	pH 7.4, 37 °C	Open record: 3-ketoacyl-CoA thiolase Tfu_0875 variant N249W/L163H, succinyl-CoA
3-ketoacyl-CoA thiolase Tfu_0875variant N249W/L163H/E217L	Thermobifida fusca	acetyl-CoA	0.0043	2.18	0.002	pH 7.4, 37 °C	Open record: 3-ketoacyl-CoA thiolase Tfu_0875 variant N249W/L163H/E217L, acetyl-CoA
3-ketoacyl-CoA thiolase Tfu_0875variant N249W/L163H/E217L	Thermobifida fusca	glutaryl-CoA	0.005	2.2	0.0023	pH 7.4, 37 °C	Open record: 3-ketoacyl-CoA thiolase Tfu_0875 variant N249W/L163H/E217L, glutaryl-CoA
3-ketoacyl-CoA thiolase Tfu_0875variant N249W/L163H/E217L	Thermobifida fusca	malonyl-CoA	0.003	2.57	0.0012	pH 7.4, 37 °C	Open record: 3-ketoacyl-CoA thiolase Tfu_0875 variant N249W/L163H/E217L, malonyl-CoA
3-ketoacyl-CoA thiolase Tfu_0875variant N249W/L163H/E217L	Thermobifida fusca	succinyl-CoA	0.0098	0.59	0.0167	pH 7.4, 37 °C	Open record: 3-ketoacyl-CoA thiolase Tfu_0875 variant N249W/L163H/E217L, succinyl-CoA