Values CatLog read from this paper
Exploring the Structure-Function Relationships in a 5-Aminolevulinic Acid Synthase and the Use of Protein Engineering to Expand its Substrate Range
PMID 39688068doi:10.1021/acs.biochem.4c00599Open in Browse
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CatLog read 21 records (51 values) from this paper: 5-aminolevulinate synthase (RpALAS) from Rhodopseudomonas palustris.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 5-aminolevulinate synthase (RpALAS) | Rhodopseudomonas palustris | succinyl-CoA | 1.2 | — | — | Open record: 5-aminolevulinate synthase (RpALAS), succinyl-CoA | |
| 5-aminolevulinate synthase (RpALAS)variant R21N | Rhodopseudomonas palustris | butyryl-CoA | 0.018 | 0.9 | 0.2 | pH 7.5, 25 °C | Open record: 5-aminolevulinate synthase (RpALAS) variant R21N, butyryl-CoA |
| 5-aminolevulinate synthase (RpALAS)variant R21N/N84L | Rhodopseudomonas palustris | butyryl-CoA | 0.049 | 1.1 | 0.044 | pH 7.5, 25 °C | Open record: 5-aminolevulinate synthase (RpALAS) variant R21N/N84L, butyryl-CoA |
| 5-aminolevulinate synthase (RpALAS)variant R21N/N84L | Rhodopseudomonas palustris | glycine | — | 3.4 | 0.014 | pH 7.5, 25 °C | Open record: 5-aminolevulinate synthase (RpALAS) variant R21N/N84L, glycine |
| 5-aminolevulinate synthase (RpALAS)variant R21N/N84L/T362V | Rhodopseudomonas palustris | butyryl-CoA | 0.37 | 0.32 | 1.2 | pH 7.5, 25 °C | Open record: 5-aminolevulinate synthase (RpALAS) variant R21N/N84L/T362V, butyryl-CoA |
| 5-aminolevulinate synthase (RpALAS)variant R21N/N84L/T362V | Rhodopseudomonas palustris | glycine | — | 0.8 | 0.5 | pH 7.5, 25 °C | Open record: 5-aminolevulinate synthase (RpALAS) variant R21N/N84L/T362V, glycine |
| 5-aminolevulinate synthase (RpALAS)variant R21N/T362V | Rhodopseudomonas palustris | butyryl-CoA | 0.099 | 1.3 | 0.076 | pH 7.5, 25 °C | Open record: 5-aminolevulinate synthase (RpALAS) variant R21N/T362V, butyryl-CoA |
| 5-aminolevulinate synthase (RpALAS)variant R21N/T362V | Rhodopseudomonas palustris | glycine | — | 6 | 0.015 | pH 7.5, 25 °C | Open record: 5-aminolevulinate synthase (RpALAS) variant R21N/T362V, glycine |
| 5-aminolevulinate synthase (RpALAS)variant R21N/T82A | Rhodopseudomonas palustris | butyryl-CoA | 0.16 | 0.33 | 0.48 | pH 7.5, 25 °C | Open record: 5-aminolevulinate synthase (RpALAS) variant R21N/T82A, butyryl-CoA |
| 5-aminolevulinate synthase (RpALAS)variant R21N/T82A | Rhodopseudomonas palustris | glycine | — | 1.7 | — | pH 7.5, 25 °C | Open record: 5-aminolevulinate synthase (RpALAS) variant R21N/T82A, glycine |
| 5-aminolevulinate synthase (RpALAS)variant R21N/T82A/T362V | Rhodopseudomonas palustris | butyryl-CoA | 0.62 | 0.15 | 4.1 | pH 7.5, 25 °C | Open record: 5-aminolevulinate synthase (RpALAS) variant R21N/T82A/T362V, butyryl-CoA |
| 5-aminolevulinate synthase (RpALAS)variant R21N/T82A/T362V | Rhodopseudomonas palustris | glycine | — | 4.4 | 0.14 | pH 7.5, 25 °C | Open record: 5-aminolevulinate synthase (RpALAS) variant R21N/T82A/T362V, glycine |
| 5-aminolevulinate synthase (RpALAS)variant R21P | Rhodopseudomonas palustris | butyryl-CoA | 0.027 | 1.4 | 0.019 | pH 7.5, 25 °C | Open record: 5-aminolevulinate synthase (RpALAS) variant R21P, butyryl-CoA |
| 5-aminolevulinate synthase (RpALAS)variant R21P/N84L | Rhodopseudomonas palustris | butyryl-CoA | 0.076 | 0.23 | 0.33 | pH 7.5, 25 °C | Open record: 5-aminolevulinate synthase (RpALAS) variant R21P/N84L, butyryl-CoA |
| 5-aminolevulinate synthase (RpALAS)variant R21P/N84L | Rhodopseudomonas palustris | glycine | — | 1.6 | 0.048 | pH 7.5, 25 °C | Open record: 5-aminolevulinate synthase (RpALAS) variant R21P/N84L, glycine |
| 5-aminolevulinate synthase (RpALAS)variant R21P/N84L/T362V | Rhodopseudomonas palustris | butyryl-CoA | 0.5 | 0.52 | 0.96 | pH 7.5, 25 °C | Open record: 5-aminolevulinate synthase (RpALAS) variant R21P/N84L/T362V, butyryl-CoA |
| 5-aminolevulinate synthase (RpALAS)variant R21P/N84L/T362V | Rhodopseudomonas palustris | glycine | — | 1.3 | 0.38 | pH 7.5, 25 °C | Open record: 5-aminolevulinate synthase (RpALAS) variant R21P/N84L/T362V, glycine |
| 5-aminolevulinate synthase (RpALAS)variant R21P/T82S/N84L | Rhodopseudomonas palustris | butyryl-CoA | 0.44 | 0.37 | 1.2 | pH 7.5, 25 °C | Open record: 5-aminolevulinate synthase (RpALAS) variant R21P/T82S/N84L, butyryl-CoA |
| 5-aminolevulinate synthase (RpALAS)variant R21P/T82S/N84L | Rhodopseudomonas palustris | glycine | — | 1 | 0.44 | pH 7.5, 25 °C | Open record: 5-aminolevulinate synthase (RpALAS) variant R21P/T82S/N84L, glycine |
| 5-aminolevulinate synthase (RpALAS)variant R21P/T82S/N84L/T362V | Rhodopseudomonas palustris | butyryl-CoA | 0.4 | 0.6 | 0.7 | pH 7.5, 25 °C | Open record: 5-aminolevulinate synthase (RpALAS) variant R21P/T82S/N84L/T362V, butyryl-CoA |
| 5-aminolevulinate synthase (RpALAS)variant R21P/T82S/N84L/T362V | Rhodopseudomonas palustris | glycine | — | 0.8 | 0.5 | pH 7.5, 25 °C | Open record: 5-aminolevulinate synthase (RpALAS) variant R21P/T82S/N84L/T362V, glycine |