Values CatLog read from this paper
Effect of loop-grafting on the activity, stability and regioselectivity of Priestia megaterium levansucrase using two inulosucrases as loop donors
PMID 40023420doi:10.1016/j.ijbiomac.2025.141584Open in Browse
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CatLog read 13 records (39 values) from this paper: Pm-SacB levansucrase from Priestia megaterium.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Pm-SacB levansucrase | Priestia megaterium | sucrose | 137.7 | 13.28 | 10.37 | pH 6.6, 25 °C | Open record: Pm-SacB levansucrase, sucrose |
| Pm-SacB levansucrase | Priestia megaterium | sucrose | 356.9 | 20.06 | 17.79 | pH 6.6, 37 °C | Open record: Pm-SacB levansucrase, sucrose |
| Pm-SacB levansucrasevariant Lj-Loop1 (Pm-SacB loop 1 residues 120-129 GDPKDSNDTS replaced by Lj-InuJ 301-306 NVNDNH) | Priestia megaterium | sucrose | 48.29 | 66.94 | 0.72 | pH 6.6, 25 °C | Open record: Pm-SacB levansucrase variant Lj-Loop1 (Pm-SacB loop 1 residues 120-129 GDPKDSNDTS replaced by Lj-InuJ 301-306 NVNDNH), sucrose |
| Pm-SacB levansucrasevariant Lj-Loop2 (Pm-SacB loop 2 residues 150-171 replaced by Lj-InuJ 327-336 PIFGLGTPVI) | Priestia megaterium | sucrose | 27.1 | 69.36 | 0.39 | pH 6.6, 25 °C | Open record: Pm-SacB levansucrase variant Lj-Loop2 (Pm-SacB loop 2 residues 150-171 replaced by Lj-InuJ 327-336 PIFGLGTPVI), sucrose |
| Pm-SacB levansucrasevariant Lj-Loop3 (Pm-SacB loop 3 residues 190-200 DYSGKQYGKQT replaced by Lj-InuJ 357-367 KVDTSDNNTNH) | Priestia megaterium | sucrose | 108.6 | 99.07 | 1.1 | pH 6.6, 37 °C | Open record: Pm-SacB levansucrase variant Lj-Loop3 (Pm-SacB loop 3 residues 190-200 DYSGKQYGKQT replaced by Lj-InuJ 357-367 KVDTSDNNTNH), sucrose |
| Pm-SacB levansucrasevariant Lj-Loop3* (grafted Lj-InuJ loop 3 plus S372N/K373R) | Priestia megaterium | sucrose | 78.68 | 48.11 | 1.63 | pH 6.6, 37 °C | Open record: Pm-SacB levansucrase variant Lj-Loop3* (grafted Lj-InuJ loop 3 plus S372N/K373R), sucrose |
| Pm-SacB levansucrasevariant Lj-Loop3-4 (Lj-InuJ loops 3 and 4 grafted into Pm-SacB) | Priestia megaterium | sucrose | 62.9 | 284.9 | 0.22 | pH 6.6, 37 °C | Open record: Pm-SacB levansucrase variant Lj-Loop3-4 (Lj-InuJ loops 3 and 4 grafted into Pm-SacB), sucrose |
| Pm-SacB levansucrasevariant Lj-Loop3-7** (grafted Lj-InuJ loops 3 and 7 plus S372N/K373R/H423Y) | Priestia megaterium | sucrose | 591 | 222.2 | 2.66 | pH 6.6, 37 °C | Open record: Pm-SacB levansucrase variant Lj-Loop3-7** (grafted Lj-InuJ loops 3 and 7 plus S372N/K373R/H423Y), sucrose |
| Pm-SacB levansucrasevariant Lj-Loop3-7-8** (grafted Lj-InuJ loops 3, 7 and 8 plus S372N/K373R/H423Y) | Priestia megaterium | sucrose | 588.0 | 264.7 | 2.22 | pH 6.6, 37 °C | Open record: Pm-SacB levansucrase variant Lj-Loop3-7-8** (grafted Lj-InuJ loops 3, 7 and 8 plus S372N/K373R/H423Y), sucrose |
| Pm-SacB levansucrasevariant Lj-Loop4 (Pm-SacB loop 4 residues 228-255 replaced by Lj-InuJ 403-418 YHYQTYDQWKETNKGA) | Priestia megaterium | sucrose | 222.9 | 245.7 | 0.91 | pH 6.6, 37 °C | Open record: Pm-SacB levansucrase variant Lj-Loop4 (Pm-SacB loop 4 residues 228-255 replaced by Lj-InuJ 403-418 YHYQTYDQWKETNKGA), sucrose |
| Pm-SacB levansucrasevariant Lj-Loop7 (Pm-SacB loop 7 residues 409-420 HMDLDPNDKTFT replaced by Lj-InuJ 588-598 TASVPANWRTA) | Priestia megaterium | sucrose | 151.2 | 336.4 | 0.45 | pH 6.6, 37 °C | Open record: Pm-SacB levansucrase variant Lj-Loop7 (Pm-SacB loop 7 residues 409-420 HMDLDPNDKTFT replaced by Lj-InuJ 588-598 TASVPANWRTA), sucrose |
| Pm-SacB levansucrasevariant Lj-Loop7-8 (Lj-InuJ loops 7 and 8 grafted into Pm-SacB) | Priestia megaterium | sucrose | 358.9 | 211.8 | 1.69 | pH 6.6, 37 °C | Open record: Pm-SacB levansucrase variant Lj-Loop7-8 (Lj-InuJ loops 7 and 8 grafted into Pm-SacB), sucrose |
| Pm-SacB levansucrasevariant Lj-Loop8 (Pm-SacB loop 8 residues 440-453 replaced by Lj-InuJ 625-631 EVAGKGM) | Priestia megaterium | sucrose | 433.0 | 19.94 | 21.71 | pH 6.6, 37 °C | Open record: Pm-SacB levansucrase variant Lj-Loop8 (Pm-SacB loop 8 residues 440-453 replaced by Lj-InuJ 625-631 EVAGKGM), sucrose |