CatLogenzyme kinetics catalog

EC 2.4.1.10: levansucrase

TransferasesOpen in Browse

CatLog read 87 records for EC 2.4.1.10 from 8 papers, for 11 organisms. Names in these papers: levansucrase (LS), Pm-SacB levansucrase, levansucrase, levansucrase (oligosaccharide producing levansucrase, OPL) and 9 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 77 records for EC 2.4.1.10 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.4.1.10 in Browse.

Papers

PaperOrganismsRecords
Computational design of Bacillus licheniformis RN-01 levansucrase for control of the chain length of levan-type fructooligosaccharidesBacillus licheniformis43
Effect of loop-grafting on the activity, stability and regioselectivity of Priestia megaterium levansucrase using two inulosucrases as loop donorsPriestia megaterium13
Selected mutations in Bacillus subtilis levansucrase semi-conserved regions affecting its biochemical propertiesBacillus subtilis9
Computational design of oligosaccharide producing levansucrase from Bacillus licheniformis RN-01 to improve its thermostability for production of levan-type fructooligosaccharides from sucroseBacillus licheniformis8
doi:10.1039/c9cy00135bBeijerinckia indica subsp. indica, Gluconobacter oxydans and 4 more6
The molecular basis of the nonprocessive elongation mechanism in levansucrasesBacillus subtilis4
Enhancing prebiotic production by engineering the levanbiose-binding site of Erwinia tasmaniensis levansucraseErwinia tasmaniensis3
doi:10.1016/j.bej.2022.108524Bacillus amyloliquefaciens1

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
levansucraseBacillus subtilissucrose164.6820.3pH 6, 37 °CPMID 18596022Open
levansucrasevariant G361FBacillus subtilissucrose57.4297.30.19pH 6, 37 °CPMID 18596022Open
levansucrasevariant H243LBacillus subtilissucrose141.510.513.5pH 6, 37 °CPMID 18596022Open
levansucrasevariant I341VBacillus subtilissucrose166.37.422.6pH 6, 37 °CPMID 18596022Open
levansucrasevariant R360KBacillus subtilissucrose170.2305.6pH 6, 37 °CPMID 18596022Open
levansucrasevariant R360SBacillus subtilissucrose13.51540.09pH 6, 37 °CPMID 18596022Open
levansucrasevariant R433ABacillus subtilissucrose87.529.32.98pH 6, 37 °CPMID 18596022Open
levansucrasevariant S164ABacillus subtilissucrose6.42.52.5pH 6, 37 °CPMID 18596022Open
levansucrasevariant Y429NBacillus subtilissucrose6.3319.40.02pH 6, 37 °CPMID 18596022Open
levansucrase (EtLsc)Erwinia tasmaniensissucrose621768.2pH 6.5, 37 °CPMID 41769041Open
levansucrase (EtLsc)variant G379PErwinia tasmaniensissucrose833670.2pH 6.5, 37 °CPMID 41769041Open
levansucrase (EtLsc)variant R377AErwinia tasmaniensissucrose619837.5pH 6.5, 37 °CPMID 41769041Open
levansucrase (Lev)Bacillus amyloliquefacienssucrose102214.7pH 6, 37 °Cdoi:10.1016/j.bej.2022.108524Open
levansucrase (LS)Bacillus licheniformissucrosetotal activity (glucose release, glucose oxidase assay)373.2——pH 6, 50 °CPMID 31437510Open
levansucrase (LS)Bacillus licheniformissucrosetotal activity (glucose release, glucose oxidase assay)—44.46—pH 6, 50 °CPMID 31437510Open
levansucrase (LS)Bacillus licheniformissucrosetotal activity (glucose release, glucose oxidase assay)——8.45pH 6, 50 °CPMID 31437510Open
levansucrase (LS)Bacillus licheniformissucrosetotal activity (glucose release, glucose oxidase assay)478.8——pH 6, 50 °CPMID 31437510Open
levansucrase (LS)Bacillus licheniformissucroseapparent kcat207.1——pH 6, 50 °CPMID 31437510Open
levansucrase (LS)Bacillus licheniformissucroseapparent Km—19.6—pH 6, 50 °CPMID 31437510Open
levansucrase (LS)Bacillus licheniformissucroseapparent kcat/Km——10.57pH 6, 50 °CPMID 31437510Open
levansucrase (LS)Bacillus licheniformissucroseapparent kcat; kcat from a Hill fit, n = 1.64197.3——pH 6, 50 °CPMID 31437510Open
levansucrase (LS)Bacillus licheniformissucroseapparent kcat672.4——pH 6, 50 °CPMID 31437510Open
levansucrase (LS)Bacillus licheniformissucroseapparent Km—1463—pH 6, 50 °CPMID 31437510Open
levansucrase (LS)Bacillus licheniformissucroseapparent kcat/Km——0.46pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant K372ABacillus licheniformissucrosetotal activity (glucose release, glucose oxidase assay)156.9193.40.81pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant K372ABacillus licheniformissucrose41.886.690.48pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant K372ABacillus licheniformissucrose99.69113.10.88pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant K372YBacillus licheniformissucrosetotal activity (glucose release, glucose oxidase assay)326.6——pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant K372YBacillus licheniformissucrosetotal activity (glucose release, glucose oxidase assay)—77.91—pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant K372YBacillus licheniformissucrosetotal activity (glucose release, glucose oxidase assay)——4.19pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant K372YBacillus licheniformissucrosetotal activity (glucose release, glucose oxidase assay)472.1——pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant K372YBacillus licheniformissucroseapparent kcat144.7——pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant K372YBacillus licheniformissucroseapparent Km—44.74—pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant K372YBacillus licheniformissucroseapparent kcat/Km——3.23pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant K372YBacillus licheniformissucroseapparent kcat; kcat from a Hill fit, n = 1.49132.3——pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant K372YBacillus licheniformissucroseapparent kcat214.0——pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant K372YBacillus licheniformissucroseapparent Km—176.7—pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant K372YBacillus licheniformissucroseapparent kcat/Km——1.21pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant N251WBacillus licheniformissucrosetotal activity (glucose release, glucose oxidase assay)1119——pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant N251WBacillus licheniformissucrosetotal activity (glucose release, glucose oxidase assay)—978.4—pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant N251WBacillus licheniformissucrosetotal activity (glucose release, glucose oxidase assay)——1.14pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant N251WBacillus licheniformissucroseapparent kcat758.8——pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant N251WBacillus licheniformissucroseapparent Km—820.8—pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant N251WBacillus licheniformissucroseapparent kcat/Km——0.92pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant N251WBacillus licheniformissucroseapparent kcat; kcat from a Hill fit, n = 1.26524.0——pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant N251WBacillus licheniformissucroseapparent kcat81.77——pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant N251WBacillus licheniformissucroseapparent Km—128.0—pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant N251WBacillus licheniformissucroseapparent kcat/Km——0.64pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant N251W/K372YBacillus licheniformissucrosetotal activity (glucose release, glucose oxidase assay)220.3——pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant N251W/K372YBacillus licheniformissucrosetotal activity (glucose release, glucose oxidase assay)—795.2—pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant N251W/K372YBacillus licheniformissucrosetotal activity (glucose release, glucose oxidase assay)——0.28pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant N251W/K372YBacillus licheniformissucroseapparent kcat; kcat from a Hill fit, n = 2.0764.91——pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant N251W/K372YBacillus licheniformissucroseapparent kcat58.24——pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant N251W/K372YBacillus licheniformissucroseapparent Km—137.6—pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant N251W/K372YBacillus licheniformissucroseapparent kcat/Km——0.42pH 6, 50 °CPMID 31437510Open
levansucrase (LS)variant N251W/K372YBacillus licheniformissucroseapparent kcat; kcat from a Hill fit, n = 0.8169.07——pH 6, 50 °CPMID 31437510Open
levansucrase (oligosaccharide producing levansucrase, OPL)variant Y246SBacillus licheniformissucroseapparent kcat105——pH 6, 50 °CPMID 32439436Open
levansucrase (oligosaccharide producing levansucrase, OPL)variant Y246SBacillus licheniformissucroseapparent Km—20.4—pH 6, 50 °CPMID 32439436Open
levansucrase (oligosaccharide producing levansucrase, OPL)variant Y246SBacillus licheniformissucroseapparent kcat/Km——5.17pH 6, 50 °CPMID 32439436Open
levansucrase (oligosaccharide producing levansucrase, OPL)variant Y246SBacillus licheniformissucroseapparent kcat; kcat from a Hill fit, n = 0.59129——pH 6, 50 °CPMID 32439436Open
levansucrase (oligosaccharide producing levansucrase, OPL)variant Y246S/K82H/N83RBacillus licheniformissucroseapparent kcat95.9——pH 6, 50 °CPMID 32439436Open
levansucrase (oligosaccharide producing levansucrase, OPL)variant Y246S/K82H/N83RBacillus licheniformissucroseapparent Km—22.9—pH 6, 50 °CPMID 32439436Open
levansucrase (oligosaccharide producing levansucrase, OPL)variant Y246S/K82H/N83RBacillus licheniformissucroseapparent kcat/Km——4.19pH 6, 50 °CPMID 32439436Open
levansucrase (oligosaccharide producing levansucrase, OPL)variant Y246S/K82H/N83RBacillus licheniformissucroseapparent kcat; kcat from a Hill fit, n = 0.57122——pH 6, 50 °CPMID 32439436Open
levansucrase LS10Gluconobacter oxydanssucrose600——pH 6, 30 °Cdoi:10.1039/c9cy00135bOpen
levansucrase LS25Beijerinckia indica subsp. indicasucrose12230.534pH 6, 30 °Cdoi:10.1039/c9cy00135bOpen
levansucrase LS29Streptococcus salivariussucrose55.496.124pH 6, 30 °Cdoi:10.1039/c9cy00135bOpen
levansucrase LS3Novosphingobium aromaticivoranssucrose317.4832.46pH 6, 30 °Cdoi:10.1039/c9cy00135bOpen
levansucrase LS6Vibrio natriegenssucrose200379.57pH 6, 30 °Cdoi:10.1039/c9cy00135bOpen
levansucrase LS8Paraburkholderia graminissucrose7.2300.246pH 6, 30 °Cdoi:10.1039/c9cy00135bOpen
levansucrase SacBBacillus subtilissucrose236.7926.3pH 6PMID 33303628Open
levansucrase SacBvariant F182ABacillus subtilissucrose—14.5—pH 6PMID 33303628Open
levansucrase SacBvariant N242ABacillus subtilissucrose—19.9—pH 6PMID 33303628Open
levansucrase SacBvariant Y237ABacillus subtilissucrose256.3——pH 6PMID 33303628Open
Pm-SacB levansucrasePriestia megateriumsucrose137.713.2810.37pH 6.6, 25 °CPMID 40023420Open
Pm-SacB levansucrasePriestia megateriumsucrose356.920.0617.79pH 6.6, 37 °CPMID 40023420Open
Pm-SacB levansucrasevariant Lj-Loop1 (Pm-SacB loop 1 residues 120-129 GDPKDSNDTS replaced by Lj-InuJ 301-306 NVNDNH)Priestia megateriumsucrose48.2966.940.72pH 6.6, 25 °CPMID 40023420Open
Pm-SacB levansucrasevariant Lj-Loop2 (Pm-SacB loop 2 residues 150-171 replaced by Lj-InuJ 327-336 PIFGLGTPVI)Priestia megateriumsucrose27.169.360.39pH 6.6, 25 °CPMID 40023420Open
Pm-SacB levansucrasevariant Lj-Loop3 (Pm-SacB loop 3 residues 190-200 DYSGKQYGKQT replaced by Lj-InuJ 357-367 KVDTSDNNTNH)Priestia megateriumsucrose108.699.071.1pH 6.6, 37 °CPMID 40023420Open
Pm-SacB levansucrasevariant Lj-Loop3* (grafted Lj-InuJ loop 3 plus S372N/K373R)Priestia megateriumsucrose78.6848.111.63pH 6.6, 37 °CPMID 40023420Open
Pm-SacB levansucrasevariant Lj-Loop3-4 (Lj-InuJ loops 3 and 4 grafted into Pm-SacB)Priestia megateriumsucrose62.9284.90.22pH 6.6, 37 °CPMID 40023420Open
Pm-SacB levansucrasevariant Lj-Loop3-7** (grafted Lj-InuJ loops 3 and 7 plus S372N/K373R/H423Y)Priestia megateriumsucrose591222.22.66pH 6.6, 37 °CPMID 40023420Open
Pm-SacB levansucrasevariant Lj-Loop3-7-8** (grafted Lj-InuJ loops 3, 7 and 8 plus S372N/K373R/H423Y)Priestia megateriumsucrose588.0264.72.22pH 6.6, 37 °CPMID 40023420Open
Pm-SacB levansucrasevariant Lj-Loop4 (Pm-SacB loop 4 residues 228-255 replaced by Lj-InuJ 403-418 YHYQTYDQWKETNKGA)Priestia megateriumsucrose222.9245.70.91pH 6.6, 37 °CPMID 40023420Open
Pm-SacB levansucrasevariant Lj-Loop7 (Pm-SacB loop 7 residues 409-420 HMDLDPNDKTFT replaced by Lj-InuJ 588-598 TASVPANWRTA)Priestia megateriumsucrose151.2336.40.45pH 6.6, 37 °CPMID 40023420Open
Pm-SacB levansucrasevariant Lj-Loop7-8 (Lj-InuJ loops 7 and 8 grafted into Pm-SacB)Priestia megateriumsucrose358.9211.81.69pH 6.6, 37 °CPMID 40023420Open
Pm-SacB levansucrasevariant Lj-Loop8 (Pm-SacB loop 8 residues 440-453 replaced by Lj-InuJ 625-631 EVAGKGM)Priestia megateriumsucrose433.019.9421.71pH 6.6, 37 °CPMID 40023420Open