Values CatLog read from this paper
Cofactor engineering for improved production of 2,4-dihydroxybutyric acid via the synthetic homoserine pathway
PMID 40051839doi:10.3389/fbioe.2025.1504785Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 6 records (18 values) from this paper: (L)-malate dehydrogenase (Ec.Mdh), engineered OHB reductase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| (L)-malate dehydrogenase (Ec.Mdh), engineered OHB reductasevariant I12V/R81A/M85Q/D86S/G179D (Ec.Mdh5Q) | Escherichia coli | 2-oxo-4-hydroxybutyrate | 74.89 | 1.84 | 44.91 | pH 7, 37 °C | Open record: (L)-malate dehydrogenase (Ec.Mdh), engineered OHB reductase variant I12V/R81A/M85Q/D86S/G179D (Ec.Mdh5Q), 2-oxo-4-hydroxybutyrate |
| (L)-malate dehydrogenase (Ec.Mdh), engineered OHB reductasevariant I12V/R81A/M85Q/D86S/G179D (Ec.Mdh5Q) | Escherichia coli | NADH | 36.48 | 0.04 | 1027 | pH 7, 37 °C | Open record: (L)-malate dehydrogenase (Ec.Mdh), engineered OHB reductase variant I12V/R81A/M85Q/D86S/G179D (Ec.Mdh5Q), NADH |
| (L)-malate dehydrogenase (Ec.Mdh), engineered OHB reductasevariant I12V/R81A/M85Q/D86S/G179D (Ec.Mdh5Q) | Escherichia coli | NADPH | 2.99 | 0.32 | 9.5 | pH 7, 37 °C | Open record: (L)-malate dehydrogenase (Ec.Mdh), engineered OHB reductase variant I12V/R81A/M85Q/D86S/G179D (Ec.Mdh5Q), NADPH |
| (L)-malate dehydrogenase (Ec.Mdh), engineered OHB reductasevariant I12V/R81A/M85Q/D86S/G179D/D34G/I35R (Ec.Mdh7Q) | Escherichia coli | 2-oxo-4-hydroxybutyrate | 83.43 | 1.08 | 80.29 | pH 7, 37 °C | Open record: (L)-malate dehydrogenase (Ec.Mdh), engineered OHB reductase variant I12V/R81A/M85Q/D86S/G179D/D34G/I35R (Ec.Mdh7Q), 2-oxo-4-hydroxybutyrate |
| (L)-malate dehydrogenase (Ec.Mdh), engineered OHB reductasevariant I12V/R81A/M85Q/D86S/G179D/D34G/I35R (Ec.Mdh7Q) | Escherichia coli | NADH | 10.79 | 0.27 | 40.35 | pH 7, 37 °C | Open record: (L)-malate dehydrogenase (Ec.Mdh), engineered OHB reductase variant I12V/R81A/M85Q/D86S/G179D/D34G/I35R (Ec.Mdh7Q), NADH |
| (L)-malate dehydrogenase (Ec.Mdh), engineered OHB reductasevariant I12V/R81A/M85Q/D86S/G179D/D34G/I35R (Ec.Mdh7Q) | Escherichia coli | NADPH | 64.03 | 0.1 | 645.5 | pH 7, 37 °C | Open record: (L)-malate dehydrogenase (Ec.Mdh), engineered OHB reductase variant I12V/R81A/M85Q/D86S/G179D/D34G/I35R (Ec.Mdh7Q), NADPH |