Values CatLog read from this paper
Defining substrate specificities of human RNA capping methyltransferases through quantitative assessment of independent yet cooperative activities
PMID 41432309doi:10.1002/pro.70428Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (11 values) from this paper: CMTR1 (cap-1 methyltransferase), CMTR1, CMTR2 (cap-2 methyltransferase) and 3 more from Homo sapiens, SARS-CoV-2.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
EC 2.1.1.56, EC 2.1.1.57, EC 2.1.1.296
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| CMTR1 | Homo sapiens | 5'-[(N7 methylated)G]pppACCCCC RNA (6 transcribed nucleotides) | 0.00383 | — | — | pH 8, 23 °C | Open record: CMTR1, 5'-[(N7 methylated)G]pppACCCCC RNA (6 transcribed nucleotides) |
| CMTR1 | Homo sapiens | 5'-[(N7 methylated)G]pppAUUAAA RNA (m7GpppAUUAAA) | 0.00361 | — | — | pH 8, 23 °C | Open record: CMTR1, 5'-[(N7 methylated)G]pppAUUAAA RNA (m7GpppAUUAAA) |
| CMTR1 (cap-1 methyltransferase) | Homo sapiens | 5'-[(N7 methylated)G]pppA[C(2'-O-ribose methylated)]CCCCCCCC-biotinylated 3' RNA | 0.0025 | — | — | Open record: CMTR1 (cap-1 methyltransferase), 5'-[(N7 methylated)G]pppA[C(2'-O-ribose methylated)]CCCCCCCC-biotinylated 3' RNA | |
| CMTR1 (cap-1 methyltransferase) | Homo sapiens | 5'-[(N7 methylated)G]pppACCCCCCCCC-biotinylated 3' RNA | 0.00283 | — | — | Open record: CMTR1 (cap-1 methyltransferase), 5'-[(N7 methylated)G]pppACCCCCCCCC-biotinylated 3' RNA | |
| CMTR1 (cap-1 methyltransferase) | Homo sapiens | 5'-GpppACCCCCCCCC-biotinylated 3' RNA | 0.0031 | — | — | Open record: CMTR1 (cap-1 methyltransferase), 5'-GpppACCCCCCCCC-biotinylated 3' RNA | |
| CMTR2 (cap-2 methyltransferase) | Homo sapiens | 5'-[(N7 methylated)G]ppp[A(2'-O-ribose methylated)]CCCCCCCCC-biotinylated 3' RNA | 9.4 × 10⁻⁴ | — | — | Open record: CMTR2 (cap-2 methyltransferase), 5'-[(N7 methylated)G]ppp[A(2'-O-ribose methylated)]CCCCCCCCC-biotinylated 3' RNA | |
| CMTR2 (cap-2 methyltransferase) | Homo sapiens | 5'-Gppp[A(2'-O-ribose methylated)]CCCCCCCCC-biotinylated 3' RNA | 8.6 × 10⁻⁵ | — | — | Open record: CMTR2 (cap-2 methyltransferase), 5'-Gppp[A(2'-O-ribose methylated)]CCCCCCCCC-biotinylated 3' RNA | |
| nsp10-nsp16 complex | SARS-CoV-2 | 5'-[(N7 methylated)G]pppA[C(2'-O-ribose methylated)]CCCCCCCC-biotinylated 3' RNA | 1.9 × 10⁻⁴ | — | — | Open record: nsp10-nsp16 complex, 5'-[(N7 methylated)G]pppA[C(2'-O-ribose methylated)]CCCCCCCC-biotinylated 3' RNA | |
| nsp14 | SARS-CoV-2 | 5'-Gppp[A(2'-O-ribose methylated)]CCCCCCCCC-biotinylated 3' RNA | 0.0336 | 7.4 × 10⁻⁵ | 450 calc. | Open record: nsp14, 5'-Gppp[A(2'-O-ribose methylated)]CCCCCCCCC-biotinylated 3' RNA | |
| RNMT (RNA guanine-N7 methyltransferase) | Homo sapiens | 5'-Gppp[A(2'-O-ribose methylated)]CCCCCCCCC-biotinylated 3' RNA | 0.0606 | — | — | Open record: RNMT (RNA guanine-N7 methyltransferase), 5'-Gppp[A(2'-O-ribose methylated)]CCCCCCCCC-biotinylated 3' RNA |