Values CatLog read from this paper
Hydrophobic interactions of a substrate with L193 on the flexible substrate-binding loop contribute to 3α-hydroxysteroid dehydrogenase/carbonyl reductase catalytic efficiency
PMID 41940686doi:10.1002/pro.70555Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 63 records (87 values) from this paper: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR), 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) from Comamonas testosteroni.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193A | Comamonas testosteroni | 2-decalol | — | — | 0.066 | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193A, 2-decalol |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193A | Comamonas testosteroni | androsterone | — | — | 1.8 × 10³ | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193A, androsterone |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193A | Comamonas testosteroni | cyclohexanol | — | — | 0.0025 | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193A, cyclohexanol |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193F | Comamonas testosteroni | 2-decalol | 0.9 | 0.906 | 1.1 | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193F, 2-decalol |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193F | Comamonas testosteroni | androsterone | 508 | 0.0065 | 8 × 10⁴ | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193F, androsterone |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193F | Comamonas testosteroni | cyclohexanol | 1 | 30 | 0.032 | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193F, cyclohexanol |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193F | Comamonas testosteroni | nicotinamide adenine dinucleotide (oxidized) | — | 0.127 | — | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193F, nicotinamide adenine dinucleotide (oxidized) |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193G | Comamonas testosteroni | 2-decalol | — | — | 0.017 | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193G, 2-decalol |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193G | Comamonas testosteroni | androsterone | — | — | 88 | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193G, androsterone |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193G | Comamonas testosteroni | cyclohexanol | — | — | 0.0041 | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193G, cyclohexanol |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193I | Comamonas testosteroni | 2-decalol | 1.3 | 1.87 | 0.7 | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193I, 2-decalol |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193I | Comamonas testosteroni | androsterone | — | — | 1.75 × 10⁴ | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193I, androsterone |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193I | Comamonas testosteroni | cyclohexanol | 2.2 | 123 | 0.018 | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193I, cyclohexanol |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193M | Comamonas testosteroni | 2-decalol | 1.2 | 1.967 | 0.6 | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193M, 2-decalol |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193M | Comamonas testosteroni | androsterone | 849 | 0.022 | 3.8 × 10⁴ | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193M, androsterone |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193M | Comamonas testosteroni | cyclohexanol | 0.9 | 20 | 0.043 | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193M, cyclohexanol |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193M | Comamonas testosteroni | nicotinamide adenine dinucleotide (oxidized) | — | 0.503 | — | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193M, nicotinamide adenine dinucleotide (oxidized) |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193P | Comamonas testosteroni | 2-decalol | — | — | 0.00119 | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193P, 2-decalol |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193P | Comamonas testosteroni | androsterone | — | — | 2.12 | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193P, androsterone |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193P | Comamonas testosteroni | cyclohexanol | — | — | 4.1 × 10⁻⁴ | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193P, cyclohexanol |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193T | Comamonas testosteroni | 2-decalol | — | — | 0.182 | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193T, 2-decalol |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193T | Comamonas testosteroni | androsterone | — | — | 5.96 × 10³ | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193T, androsterone |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193T | Comamonas testosteroni | cyclohexanol | — | — | 0.0055 | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193T, cyclohexanol |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193V | Comamonas testosteroni | 2-decalol | — | — | 0.48 | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193V, 2-decalol |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193V | Comamonas testosteroni | androsterone | 798 | 0.04 | 2 × 10⁴ | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193V, androsterone |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193V | Comamonas testosteroni | cyclohexanol | — | — | 0.0124 | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193V, cyclohexanol |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193V | Comamonas testosteroni | nicotinamide adenine dinucleotide (oxidized) | — | 0.498 | — | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193V, nicotinamide adenine dinucleotide (oxidized) |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193W | Comamonas testosteroni | 2-decalol | — | — | 0.3 | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193W, 2-decalol |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193W | Comamonas testosteroni | androsterone | 124 | 0.02 | 6.2 × 10³ | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193W, androsterone |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193W | Comamonas testosteroni | cyclohexanol | — | — | 0.0161 | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193W, cyclohexanol |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant R201A | Comamonas testosteroni | androsterone | 407 | 0.006 | 6.73 × 10⁴ | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant R201A, androsterone |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant R201A | Comamonas testosteroni | nicotinamide adenine dinucleotide (oxidized) | — | 0.311 | — | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant R201A, nicotinamide adenine dinucleotide (oxidized) |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant Y202A | Comamonas testosteroni | androsterone | 228 | 0.048 | 4.8 × 10³ | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant Y202A, androsterone |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193A | Comamonas testosteroni | 2-decalol | — | — | 0.066 | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193A, 2-decalol |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193A | Comamonas testosteroni | androsterone | — | — | 1.8 × 10³ | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193A, androsterone |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193A | Comamonas testosteroni | cyclohexanol | — | — | 0.0025 | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193A, cyclohexanol |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193F | Comamonas testosteroni | 2-decalol | — | — | 1.1 | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193F, 2-decalol |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193F | Comamonas testosteroni | androsterone | — | — | 8 × 10⁴ | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193F, androsterone |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193F | Comamonas testosteroni | cyclohexanol | — | — | 0.032 | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193F, cyclohexanol |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193G | Comamonas testosteroni | 2-decalol | — | — | 0.017 | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193G, 2-decalol |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193G | Comamonas testosteroni | androsterone | — | — | 88 | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193G, androsterone |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193G | Comamonas testosteroni | cyclohexanol | — | — | 0.0041 | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193G, cyclohexanol |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193I | Comamonas testosteroni | 2-decalol | — | — | 0.7 | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193I, 2-decalol |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193I | Comamonas testosteroni | androsterone | — | — | 1.75 × 10⁴ | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193I, androsterone |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193I | Comamonas testosteroni | cyclohexanol | — | — | 0.018 | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193I, cyclohexanol |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193M | Comamonas testosteroni | 2-decalol | — | — | 0.6 | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193M, 2-decalol |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193M | Comamonas testosteroni | androsterone | — | — | 3.8 × 10⁴ | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193M, androsterone |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193M | Comamonas testosteroni | cyclohexanol | — | — | 0.043 | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193M, cyclohexanol |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193P | Comamonas testosteroni | 2-decalol | — | — | 0.00119 | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193P, 2-decalol |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193P | Comamonas testosteroni | androsterone | — | — | 2.12 | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193P, androsterone |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193P | Comamonas testosteroni | cyclohexanol | — | — | 4.1 × 10⁻⁴ | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193P, cyclohexanol |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193T | Comamonas testosteroni | 2-decalol | — | — | 0.182 | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193T, 2-decalol |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193T | Comamonas testosteroni | androsterone | — | — | 5.96 × 10³ | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193T, androsterone |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193T | Comamonas testosteroni | cyclohexanol | — | — | 0.0055 | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193T, cyclohexanol |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193V | Comamonas testosteroni | 2-decalol | — | — | 0.48 | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193V, 2-decalol |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193V | Comamonas testosteroni | androsteroneTable 1 | — | — | 2 × 10⁴ | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193V, androsterone |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193V | Comamonas testosteroni | androsteronein the text | — | 0.04 | — | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193V, androsterone |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193V | Comamonas testosteroni | cyclohexanol | — | — | 0.0124 | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193V, cyclohexanol |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193W | Comamonas testosteroni | 2-decalol | — | — | 0.3 | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193W, 2-decalol |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193W | Comamonas testosteroni | androsterone | — | — | 6.2 × 10³ | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193W, androsterone |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant L193W | Comamonas testosteroni | cyclohexanol | — | — | 0.0161 | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant L193W, cyclohexanol |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant R201A | Comamonas testosteroni | androsterone | — | — | 6.73 × 10⁴ | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant R201A, androsterone |
| 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR)variant Y202A | Comamonas testosteroni | androsterone | — | — | 4.8 × 10³ | pH 10.5, 25 °C | Open record: 3α-hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) variant Y202A, androsterone |