CatLogenzyme kinetics catalog

Values CatLog read from this paper

Cobamides and ribonucleotide reduction. V. A kinetic study of the ribonucleoside triphosphate reductase of Lactobacillus leichmannii

PMID 4381877doi:10.1016/s0021-9258(18)95929-6Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 15 records (15 values) from this paper: ribonucleoside triphosphate reductase from Lactobacillus leichmannii.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.17.4.2

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
ribonucleoside triphosphate reductaseLactobacillus leichmannii5'-deoxyadenosylcobalamin2 mM GTP; cobamide coenzyme varied; cosubstrate GTP—8 × 10⁻⁴—pH 7.5, 37 °COpen
ribonucleoside triphosphate reductaseLactobacillus leichmannii5'-deoxyadenosylcobalamin1.0 mM GTP; DBC coenzyme varied 0.2-2 µM; cosubstrate GTP—9 × 10⁻⁴—pH 7.5, 37 °COpen
ribonucleoside triphosphate reductaseLactobacillus leichmannii5'-deoxyadenosylcobalamin0.1 mM GTP; DBC coenzyme varied 0.2-2 µM; cosubstrate GTP—0.0028—pH 7.5, 37 °COpen
ribonucleoside triphosphate reductaseLactobacillus leichmannii5'-deoxyadenosylcobalamin2 mM CTP; no deoxyribonucleotide activator; cosubstrate CTP—0.0055—pH 7.5, 37 °COpen
ribonucleoside triphosphate reductaseLactobacillus leichmannii5'-deoxyadenosylcobalamin2 mM ATP; no deoxyribonucleotide activator; cosubstrate ATP—0.0083—pH 7.5, 37 °COpen
ribonucleoside triphosphate reductaseLactobacillus leichmannii5'-deoxyadenosylcobalamin8 µM thioredoxin; 12 µg thioredoxin reductase—4.5 × 10⁻⁴—pH 7.5, 37 °COpen
ribonucleoside triphosphate reductaseLactobacillus leichmanniiadenosine 5'-triphosphate40 µM DBC coenzyme; no deoxyribonucleotide activator—0.8—pH 7.5, 37 °COpen
ribonucleoside triphosphate reductaseLactobacillus leichmanniiadenosine 5'-triphosphate4 µM DBC coenzyme; with 1 mM dGTP (activator); ATP varied—0.22—pH 7.5, 37 °COpen
ribonucleoside triphosphate reductaseLactobacillus leichmanniiadenosyl-5'-deoxyadenosylcobamide—0.0028—pH 7.5, 37 °COpen
ribonucleoside triphosphate reductaseLactobacillus leichmanniianilide of 5'-deoxyadenosylcobalamin—0.0033—pH 7.5, 37 °COpen
ribonucleoside triphosphate reductaseLactobacillus leichmanniibenzimidazolyl-5'-deoxyadenosylcobamide—8 × 10⁻⁴—pH 7.5, 37 °COpen
ribonucleoside triphosphate reductaseLactobacillus leichmanniicytidine 5'-triphosphate40 µM DBC coenzyme; no deoxyribonucleotide activator—0.36—pH 7.5, 37 °COpen
ribonucleoside triphosphate reductaseLactobacillus leichmanniicytidine 5'-triphosphate13 µM thioredoxin; 15 µg thioredoxin reductase; CTP varied—0.13—pH 7.5, 37 °COpen
ribonucleoside triphosphate reductaseLactobacillus leichmanniiguanosine 5'-triphosphate—0.24—pH 7.5, 37 °COpen
ribonucleoside triphosphate reductaseLactobacillus leichmanniithioredoxin (E. coli)—0.004—pH 7.5, 37 °COpen