Values CatLog read from this paper

Locations and functional roles of conserved lysine residues in Salmonella typhimurium orotate phosphoribosyltransferase

PMID 7545005doi:10.1021/bi00034a007Open in Browse

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CatLog read 60 records (162 values) from this paper: orotate phosphoribosyltransferase from Salmonella typhimurium.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.4.2.10

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
orotate phosphoribosyltransferase	Salmonella typhimurium	alpha-D-5-phosphoribosyl-1-pyrophosphate	—	0.049	690	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase, alpha-D-5-phosphoribosyl-1-pyrophosphate
orotate phosphoribosyltransferase	Salmonella typhimurium	imidodiphosphateanalog 0.25-20 mM	35	0.94	38	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase, imidodiphosphate
orotate phosphoribosyltransferase	Salmonella typhimurium	imidodiphosphatepyrophosphate-analog series	35	0.9	98	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase, imidodiphosphate
orotate phosphoribosyltransferase	Salmonella typhimurium	orotic acidforward reaction (OMP synthesis); 75 mM Tris-HCl; 6 mM MgCl2	34	0.026	1.3 × 10³	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase, orotic acid
orotate phosphoribosyltransferase	Salmonella typhimurium	orotic acidforward reaction	32	0.028	1.14 × 10³	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase, orotic acid
orotate phosphoribosyltransferase	Salmonella typhimurium	orotic acid 6-methyl ester	3.5	0.182	19	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase, orotic acid 6-methyl ester
orotate phosphoribosyltransferase	Salmonella typhimurium	orotidine 5'-monophosphate	18	0.005	3.6 × 10³	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase, orotidine 5'-monophosphate
orotate phosphoribosyltransferase	Salmonella typhimurium	phosphonoacetate	0.04	1.9	0.06	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase, phosphonoacetate
orotate phosphoribosyltransferase	Salmonella typhimurium	phosphonoacetic acid	0.04	1.85	0.02	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase, phosphonoacetic acid
orotate phosphoribosyltransferase	Salmonella typhimurium	pyrophosphate80 mM Tris-HCl; MgCl2 kept 1 mM in excess over PPi	—	0.033	550	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase, pyrophosphate
orotate phosphoribosyltransferase	Salmonella typhimurium	pyrophosphatepyrophosphate-analog series	18	0.033	1.4 × 10³	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase, pyrophosphate
orotate phosphoribosyltransferase	Salmonella typhimurium	uracil	0.9	3.16	0.3	30 °C	Open record: orotate phosphoribosyltransferase, uracil
orotate phosphoribosyltransferasevariant K100A	Salmonella typhimurium	alpha-D-5-phosphoribosyl-1-pyrophosphate	—	0.179	64	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K100A, alpha-D-5-phosphoribosyl-1-pyrophosphate
orotate phosphoribosyltransferasevariant K100A	Salmonella typhimurium	imidodiphosphate	0.7	0.4	4	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K100A, imidodiphosphate
orotate phosphoribosyltransferasevariant K100A	Salmonella typhimurium	orotic acid	12	0.072	160	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K100A, orotic acid
orotate phosphoribosyltransferasevariant K100A	Salmonella typhimurium	orotidine 5'-monophosphate	12	0.013	830	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K100A, orotidine 5'-monophosphate
orotate phosphoribosyltransferasevariant K100A	Salmonella typhimurium	phosphonoacetate	0.09	1.8	0.1	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K100A, phosphonoacetate
orotate phosphoribosyltransferasevariant K100A	Salmonella typhimurium	pyrophosphate80 mM Tris-HCl; MgCl2 kept 1 mM in excess over PPi	—	0.958	11	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K100A, pyrophosphate
orotate phosphoribosyltransferasevariant K100A	Salmonella typhimurium	pyrophosphatepyrophosphate-analog series	11	0.96	150	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K100A, pyrophosphate
orotate phosphoribosyltransferasevariant K103A	Salmonella typhimurium	alpha-D-5-phosphoribosyl-1-pyrophosphate	—	0.285	0.2	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K103A, alpha-D-5-phosphoribosyl-1-pyrophosphate
orotate phosphoribosyltransferasevariant K103A	Salmonella typhimurium	orotic acidforward reaction (OMP synthesis); 75 mM Tris-HCl; 6 mM MgCl2	0.05	0.042	1	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K103A, orotic acid
orotate phosphoribosyltransferasevariant K103A	Salmonella typhimurium	orotic acidforward reaction	0.06	0.043	1.3	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K103A, orotic acid
orotate phosphoribosyltransferasevariant K103A	Salmonella typhimurium	orotic acid 6-methyl ester	0.02	0.041	0.4	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K103A, orotic acid 6-methyl ester
orotate phosphoribosyltransferasevariant K103A	Salmonella typhimurium	orotidine 5'-monophosphate	0.05	0.02	2	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K103A, orotidine 5'-monophosphate
orotate phosphoribosyltransferasevariant K103A	Salmonella typhimurium	pyrophosphate	—	0.146	0.3	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K103A, pyrophosphate
orotate phosphoribosyltransferasevariant K103Q	Salmonella typhimurium	alpha-D-5-phosphoribosyl-1-pyrophosphate	—	0.12	1	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K103Q, alpha-D-5-phosphoribosyl-1-pyrophosphate
orotate phosphoribosyltransferasevariant K103Q	Salmonella typhimurium	imidodiphosphate	0.05	0.4	0.3	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K103Q, imidodiphosphate
orotate phosphoribosyltransferasevariant K103Q	Salmonella typhimurium	orotic acid	0.13	0.034	4	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K103Q, orotic acid
orotate phosphoribosyltransferasevariant K103Q	Salmonella typhimurium	orotidine 5'-monophosphate	0.05	0.0034	15	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K103Q, orotidine 5'-monophosphate
orotate phosphoribosyltransferasevariant K103Q	Salmonella typhimurium	pyrophosphate80 mM Tris-HCl; MgCl2 kept 1 mM in excess over PPi	—	0.144	0.3	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K103Q, pyrophosphate
orotate phosphoribosyltransferasevariant K103Q	Salmonella typhimurium	pyrophosphatepyrophosphate-analog series	0.05	0.15	2.4	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K103Q, pyrophosphate
orotate phosphoribosyltransferasevariant K19Q	Salmonella typhimurium	alpha-D-5-phosphoribosyl-1-pyrophosphate	—	0.096	320	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K19Q, alpha-D-5-phosphoribosyl-1-pyrophosphate
orotate phosphoribosyltransferasevariant K19Q	Salmonella typhimurium	orotic acid	31	0.078	400	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K19Q, orotic acid
orotate phosphoribosyltransferasevariant K19Q	Salmonella typhimurium	orotidine 5'-monophosphate	12	0.018	830	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K19Q, orotidine 5'-monophosphate
orotate phosphoribosyltransferasevariant K19Q	Salmonella typhimurium	pyrophosphate	—	0.05	300	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K19Q, pyrophosphate
orotate phosphoribosyltransferasevariant K26A	Salmonella typhimurium	alpha-D-5-phosphoribosyl-1-pyrophosphate	—	0.05	175	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K26A, alpha-D-5-phosphoribosyl-1-pyrophosphate
orotate phosphoribosyltransferasevariant K26A	Salmonella typhimurium	orotic acidforward reaction (OMP synthesis); 75 mM Tris-HCl; 6 mM MgCl2	8.7	0.07	125	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K26A, orotic acid
orotate phosphoribosyltransferasevariant K26A	Salmonella typhimurium	orotic acidforward reaction	10	0.067	150	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K26A, orotic acid
orotate phosphoribosyltransferasevariant K26A	Salmonella typhimurium	orotidine 5'-monophosphate	9.6	0.019	500	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K26A, orotidine 5'-monophosphate
orotate phosphoribosyltransferasevariant K26A	Salmonella typhimurium	pyrophosphate	—	0.074	130	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K26A, pyrophosphate
orotate phosphoribosyltransferasevariant K26A	Salmonella typhimurium	uracil	0.7	3.71	0.2	30 °C	Open record: orotate phosphoribosyltransferase variant K26A, uracil
orotate phosphoribosyltransferasevariant K26Q	Salmonella typhimurium	alpha-D-5-phosphoribosyl-1-pyrophosphate	—	0.055	56	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K26Q, alpha-D-5-phosphoribosyl-1-pyrophosphate
orotate phosphoribosyltransferasevariant K26Q	Salmonella typhimurium	imidodiphosphate	0.12	1.1	0.3	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K26Q, imidodiphosphate
orotate phosphoribosyltransferasevariant K26Q	Salmonella typhimurium	orotic acidforward reaction (OMP synthesis); 75 mM Tris-HCl; 6 mM MgCl2	3.1	0.065	48	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K26Q, orotic acid
orotate phosphoribosyltransferasevariant K26Q	Salmonella typhimurium	orotic acidforward reaction	3.3	0.07	48	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K26Q, orotic acid
orotate phosphoribosyltransferasevariant K26Q	Salmonella typhimurium	orotic acid 6-methyl ester	16	0.082	192	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K26Q, orotic acid 6-methyl ester
orotate phosphoribosyltransferasevariant K26Q	Salmonella typhimurium	orotidine 5'-monophosphate	1.3	0.037	34	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K26Q, orotidine 5'-monophosphate
orotate phosphoribosyltransferasevariant K26Q	Salmonella typhimurium	phosphonoacetate	0.13	3.6	0.09	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K26Q, phosphonoacetate
orotate phosphoribosyltransferasevariant K26Q	Salmonella typhimurium	pyrophosphate80 mM Tris-HCl; MgCl2 kept 1 mM in excess over PPi	—	0.111	11	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K26Q, pyrophosphate
orotate phosphoribosyltransferasevariant K26Q	Salmonella typhimurium	pyrophosphatepyrophosphate-analog series	1.3	0.11	30	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K26Q, pyrophosphate
orotate phosphoribosyltransferasevariant K26Q	Salmonella typhimurium	uracil	0.7	1.81	0.4	30 °C	Open record: orotate phosphoribosyltransferase variant K26Q, uracil
orotate phosphoribosyltransferasevariant K73A	Salmonella typhimurium	alpha-D-5-phosphoribosyl-1-pyrophosphate	—	0.394	0.7	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K73A, alpha-D-5-phosphoribosyl-1-pyrophosphate
orotate phosphoribosyltransferasevariant K73A	Salmonella typhimurium	orotic acid	0.3	0.029	9	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K73A, orotic acid
orotate phosphoribosyltransferasevariant K73A	Salmonella typhimurium	orotidine 5'-monophosphate	0.3	0.011	28	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K73A, orotidine 5'-monophosphate
orotate phosphoribosyltransferasevariant K73A	Salmonella typhimurium	pyrophosphate80 mM Tris-HCl; MgCl2 kept 1 mM in excess over PPi	—	0.182	2	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K73A, pyrophosphate
orotate phosphoribosyltransferasevariant K73A	Salmonella typhimurium	pyrophosphatepyrophosphate-analog series	0.3	0.18	2	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K73A, pyrophosphate
orotate phosphoribosyltransferasevariant K73Q	Salmonella typhimurium	alpha-D-5-phosphoribosyl-1-pyrophosphate	—	0.576	1	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K73Q, alpha-D-5-phosphoribosyl-1-pyrophosphate
orotate phosphoribosyltransferasevariant K73Q	Salmonella typhimurium	orotic acid	0.6	0.031	19	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K73Q, orotic acid
orotate phosphoribosyltransferasevariant K73Q	Salmonella typhimurium	orotidine 5'-monophosphate	0.3	0.013	24	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K73Q, orotidine 5'-monophosphate
orotate phosphoribosyltransferasevariant K73Q	Salmonella typhimurium	pyrophosphate	—	0.102	3	pH 8, 30 °C	Open record: orotate phosphoribosyltransferase variant K73Q, pyrophosphate