CatLogenzyme kinetics catalog

Values CatLog read from this paper

Engineering a domain-locking disulfide into a bacterial malate dehydrogenase produces a redox-sensitive enzyme

PMID 7647229doi:10.1016/s0006-3495(95)80430-3Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 8 records (12 values) from this paper: malate dehydrogenase (NAD-linked) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.1.1.37

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
malate dehydrogenase (NAD-linked)Escherichia coliNADH9000.0611.5 × 10⁴ calc.pH 7.5, 25 °COpen
malate dehydrogenase (NAD-linked)Escherichia colioxaloacetate—0.049—pH 7.5, 25 °COpen
malate dehydrogenase (NAD-linked)variant L305CEscherichia coliNADH1.1 × 10³0.0871.3 × 10⁴ calc.pH 7.5, 25 °COpen
malate dehydrogenase (NAD-linked)variant L305CEscherichia colioxaloacetate—0.044—pH 7.5, 25 °COpen
malate dehydrogenase (NAD-linked)variant N122C/L305CEscherichia coliNADH1.1 × 10³0.0562.0 × 10⁴ calc.pH 7.5, 25 °COpen
malate dehydrogenase (NAD-linked)variant N122C/L305CEscherichia coliNADH1300.0522.5 × 10³ calc.pH 7.5, 25 °COpen
malate dehydrogenase (NAD-linked)variant N122C/L305CEscherichia colioxaloacetate—0.34—pH 7.5, 25 °COpen
malate dehydrogenase (NAD-linked)variant N122C/L305CEscherichia colioxaloacetate—0.58—pH 7.5, 25 °COpen