Values CatLog read from this paper
Improved specificity toward substrates with positively charged side chains by site-directed mutagenesis of the L-lactate dehydrogenase of Bacillus stearothermophilus
PMID 7703235doi:10.1021/bi00013a011Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 25 records (75 values) from this paper: L-lactate dehydrogenase (BSLDH) from Bacillus stearothermophilus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| L-lactate dehydrogenase (BSLDH) | Bacillus stearothermophilus | 2-oxoadipate | 5.3 | 1.6 | 3.3 | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH), 2-oxoadipate |
| L-lactate dehydrogenase (BSLDH) | Bacillus stearothermophilus | 4-amino-2-oxobutanoate | 0.3 | 38 | 0.0079 | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH), 4-amino-2-oxobutanoate |
| L-lactate dehydrogenase (BSLDH) | Bacillus stearothermophilus | 6-amino-2-oxohexanoate | 0.008 | 22 | 3.6 × 10⁻⁴ | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH), 6-amino-2-oxohexanoate |
| L-lactate dehydrogenase (BSLDH)variant Q102D | Bacillus stearothermophilus | 2-oxoadipate | 3.6 | 3 | 1.2 | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH) variant Q102D, 2-oxoadipate |
| L-lactate dehydrogenase (BSLDH)variant Q102D | Bacillus stearothermophilus | 2-oxobutanoate | 42 | 5.1 | 8.2 | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH) variant Q102D, 2-oxobutanoate |
| L-lactate dehydrogenase (BSLDH)variant Q102D | Bacillus stearothermophilus | 2-oxoglutarate | 2.3 | 10.9 | 0.21 | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH) variant Q102D, 2-oxoglutarate |
| L-lactate dehydrogenase (BSLDH)variant Q102D | Bacillus stearothermophilus | 2-oxohexanoate | 16 | 3 | 5.3 | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH) variant Q102D, 2-oxohexanoate |
| L-lactate dehydrogenase (BSLDH)variant Q102D | Bacillus stearothermophilus | 2-oxomalonate | 9.5 | 11.4 | 0.83 | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH) variant Q102D, 2-oxomalonate |
| L-lactate dehydrogenase (BSLDH)variant Q102D | Bacillus stearothermophilus | 2-oxopentanoate | 16.2 | 7.3 | 2.2 | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH) variant Q102D, 2-oxopentanoate |
| L-lactate dehydrogenase (BSLDH)variant Q102D | Bacillus stearothermophilus | 4-amino-2-oxobutanoate | 0.6 | 19 | 0.032 | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH) variant Q102D, 4-amino-2-oxobutanoate |
| L-lactate dehydrogenase (BSLDH)variant Q102D | Bacillus stearothermophilus | 6-amino-2-oxohexanoate | 0.03 | 14 | 0.0021 | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH) variant Q102D, 6-amino-2-oxohexanoate |
| L-lactate dehydrogenase (BSLDH)variant Q102D | Bacillus stearothermophilus | hydroxypyruvate | 27.2 | 11 | 2.5 | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH) variant Q102D, hydroxypyruvate |
| L-lactate dehydrogenase (BSLDH)variant Q102D | Bacillus stearothermophilus | phenylpyruvate | 78 | 2.1 | 37 | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH) variant Q102D, phenylpyruvate |
| L-lactate dehydrogenase (BSLDH)variant Q102D | Bacillus stearothermophilus | pyruvate | 50 | 3.5 | 14 | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH) variant Q102D, pyruvate |
| L-lactate dehydrogenase (BSLDH)variant Q102E | Bacillus stearothermophilus | 2-oxoadipate | 3.2 | 4.7 | 0.68 | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH) variant Q102E, 2-oxoadipate |
| L-lactate dehydrogenase (BSLDH)variant Q102E | Bacillus stearothermophilus | 2-oxobutanoate | 53 | 3.2 | 17 | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH) variant Q102E, 2-oxobutanoate |
| L-lactate dehydrogenase (BSLDH)variant Q102E | Bacillus stearothermophilus | 2-oxoglutarate | 3 | 12.1 | 0.25 | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH) variant Q102E, 2-oxoglutarate |
| L-lactate dehydrogenase (BSLDH)variant Q102E | Bacillus stearothermophilus | 2-oxohexanoate | 23 | 4.7 | 4.9 | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH) variant Q102E, 2-oxohexanoate |
| L-lactate dehydrogenase (BSLDH)variant Q102E | Bacillus stearothermophilus | 2-oxomalonate | 28 | 10.2 | 2.8 | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH) variant Q102E, 2-oxomalonate |
| L-lactate dehydrogenase (BSLDH)variant Q102E | Bacillus stearothermophilus | 2-oxopentanoate | 13.6 | 6.6 | 2.1 | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH) variant Q102E, 2-oxopentanoate |
| L-lactate dehydrogenase (BSLDH)variant Q102E | Bacillus stearothermophilus | 4-amino-2-oxobutanoate | 1.5 | 10 | 0.15 | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH) variant Q102E, 4-amino-2-oxobutanoate |
| L-lactate dehydrogenase (BSLDH)variant Q102E | Bacillus stearothermophilus | 6-amino-2-oxohexanoate | 0.1 | 11 | 0.009 | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH) variant Q102E, 6-amino-2-oxohexanoate |
| L-lactate dehydrogenase (BSLDH)variant Q102E | Bacillus stearothermophilus | hydroxypyruvate | 52.5 | 3.2 | 16 | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH) variant Q102E, hydroxypyruvate |
| L-lactate dehydrogenase (BSLDH)variant Q102E | Bacillus stearothermophilus | phenylpyruvate | 46 | 1.5 | 31 | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH) variant Q102E, phenylpyruvate |
| L-lactate dehydrogenase (BSLDH)variant Q102E | Bacillus stearothermophilus | pyruvate | 150 | 0.65 | 230 | pH 6, 25 °C | Open record: L-lactate dehydrogenase (BSLDH) variant Q102E, pyruvate |