CatLogenzyme kinetics catalog

Values CatLog read from this paper

Mutational analysis of the catalytic residues lysine 230 and tyrosine 160 in the NADP(+)-dependent isocitrate dehydrogenase from Escherichia coli

PMID 7819221doi:10.1021/bi00001a046Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 11 records (33 values) from this paper: NADP+-dependent isocitrate dehydrogenase from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.42

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
NADP+-dependent isocitrate dehydrogenaseEscherichia coli(2R,3S)-isocitrate (Ds-threo-isocitrate)76.20.01146.71 × 10³Open
NADP+-dependent isocitrate dehydrogenaseEscherichia coliD-2-hydroxyglutarate7.24 × 10⁻⁴32.41 × 10⁻⁴pH 7.8Open
NADP+-dependent isocitrate dehydrogenaseEscherichia colioxalosuccinate1.410.344.15Open
NADP+-dependent isocitrate dehydrogenaseEscherichia colioxalosuccinate20.60.2293.6pH 7.8Open
NADP+-dependent isocitrate dehydrogenasevariant K230MEscherichia coli(2R,3S)-isocitrate (Ds-threo-isocitrate)0.856.030.141Open
NADP+-dependent isocitrate dehydrogenasevariant K230MEscherichia coliD-2-hydroxyglutarate2.13 × 10⁻⁴12.91.65 × 10⁻⁵pH 7.8Open
NADP+-dependent isocitrate dehydrogenasevariant K230MEscherichia colioxalosuccinate0.36820.184Open
NADP+-dependent isocitrate dehydrogenasevariant Y160FEscherichia coli(2R,3S)-isocitrate (Ds-threo-isocitrate)0.3110.009632.3Open
NADP+-dependent isocitrate dehydrogenasevariant Y160FEscherichia coliD-2-hydroxyglutarate6.7 × 10⁻⁴10.66.32 × 10⁻⁵pH 7.8Open
NADP+-dependent isocitrate dehydrogenasevariant Y160FEscherichia colioxalosuccinate0.0530.5620.0943Open
NADP+-dependent isocitrate dehydrogenasevariant Y160FEscherichia colioxalosuccinate36.20.22165pH 7.8Open