Values CatLog read from this paper
Expression of recombinant human phenylalanine hydroxylase as fusion protein in Escherichia coli circumvents proteolytic degradation by host cell proteases. Isolation and characterization of the…
PMID 7887915doi:10.1042/bj3060589Open in Browse
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CatLog read 11 records (11 values) from this paper: phenylalanine hydroxylase (hPAH) from Homo sapiens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| phenylalanine hydroxylase (hPAH) | Homo sapiens | (6R)-L-erythro-tetrahydrobiopterintetrameric form (365 kDa) from size-exclusion chromatography | — | 0.031 | — | pH 7, 25 °C | Open record: phenylalanine hydroxylase (hPAH), (6R)-L-erythro-tetrahydrobiopterin |
| phenylalanine hydroxylase (hPAH) | Homo sapiens | (6R)-L-erythro-tetrahydrobiopterinTable 4 | — | 0.025 | — | pH 7, 25 °C | Open record: phenylalanine hydroxylase (hPAH), (6R)-L-erythro-tetrahydrobiopterin |
| phenylalanine hydroxylase (hPAH) | Homo sapiens | (6R)-L-erythro-tetrahydrobiopterinTable 4 | — | 0.029 | — | pH 7, 25 °C | Open record: phenylalanine hydroxylase (hPAH), (6R)-L-erythro-tetrahydrobiopterin |
| phenylalanine hydroxylase (hPAH) | Homo sapiens | (6R)-L-erythro-tetrahydrobiopterinapproximate Km; in the text | — | 0.018 | — | pH 7, 25 °C | Open record: phenylalanine hydroxylase (hPAH), (6R)-L-erythro-tetrahydrobiopterin |
| phenylalanine hydroxylase (hPAH) | Homo sapiens | 6-methyl-5,6,7,8-tetrahydropterintetrameric form (365 kDa) from size-exclusion chromatography | — | 0.073 | — | pH 7, 25 °C | Open record: phenylalanine hydroxylase (hPAH), 6-methyl-5,6,7,8-tetrahydropterin |
| phenylalanine hydroxylase (hPAH) | Homo sapiens | 6-methyl-5,6,7,8-tetrahydropterin | — | 0.088 | — | pH 7, 25 °C | Open record: phenylalanine hydroxylase (hPAH), 6-methyl-5,6,7,8-tetrahydropterin |
| phenylalanine hydroxylase (hPAH) | Homo sapiens | L-phenylalanine75 µM (6R)-tetrahydrobiopterin (BH4); cosubstrate BH4 | — | 0.194 | — | pH 7, 25 °C | Open record: phenylalanine hydroxylase (hPAH), L-phenylalanine |
| phenylalanine hydroxylase (hPAH) | Homo sapiens | L-phenylalanine500 µM 6-methyltetrahydropterin (6-MPH4); cosubstrate 6-MPH4 | — | 0.504 | — | pH 7, 25 °C | Open record: phenylalanine hydroxylase (hPAH), L-phenylalanine |
| phenylalanine hydroxylase (hPAH) | Homo sapiens | L-phenylalanine75 µM (6R)-tetrahydrobiopterin (BH4); cosubstrate BH4 | — | 0.236 | — | pH 7, 25 °C | Open record: phenylalanine hydroxylase (hPAH), L-phenylalanine |
| phenylalanine hydroxylase (hPAH) | Homo sapiens | L-phenylalanine75 µM (6R)-tetrahydrobiopterin (BH4); cosubstrate BH4 | — | 0.175 | — | pH 7, 25 °C | Open record: phenylalanine hydroxylase (hPAH), L-phenylalanine |
| phenylalanine hydroxylase (hPAH) | Homo sapiens | L-phenylalanine500 µM 6-methyltetrahydropterin (6-MPH4); cosubstrate 6-MPH4 | — | 0.382 | — | pH 7, 25 °C | Open record: phenylalanine hydroxylase (hPAH), L-phenylalanine |