Values CatLog read from this paper
Functional analysis of Gln-237 mutants of HhaI methyltransferase
PMID 7899082doi:10.1093/nar/23.4.620Open in Browse
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CatLog read 12 records (24 values) from this paper: HhaI DNA (cytosine-5) methyltransferase (M.HhaI).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| HhaI DNA (cytosine-5) methyltransferase (M.HhaI) | — | 37mer DNA duplex (oligonucleotides 11 and 12, single GCGC site) | 0.0533 | 6.9 × 10⁻⁵ | 770 | pH 7.4, 37 °C | Open record: HhaI DNA (cytosine-5) methyltransferase (M.HhaI), 37mer DNA duplex (oligonucleotides 11 and 12, single GCGC site) |
| HhaI DNA (cytosine-5) methyltransferase (M.HhaI) | — | S-adenosyl-L-methionine | — | 1.5 × 10⁻⁵ | — | pH 7.4, 37 °C | Open record: HhaI DNA (cytosine-5) methyltransferase (M.HhaI), S-adenosyl-L-methionine |
| HhaI DNA (cytosine-5) methyltransferase (M.HhaI)variant I2L/Q237A | — | 37mer DNA duplex (oligonucleotides 11 and 12, single GCGC site) | 0.0058 | 8.1 × 10⁻⁵ | 70 | pH 7.4, 37 °C | Open record: HhaI DNA (cytosine-5) methyltransferase (M.HhaI) variant I2L/Q237A, 37mer DNA duplex (oligonucleotides 11 and 12, single GCGC site) |
| HhaI DNA (cytosine-5) methyltransferase (M.HhaI)variant I2L/Q237A | — | S-adenosyl-L-methionine | — | 1.7 × 10⁻⁵ | — | pH 7.4, 37 °C | Open record: HhaI DNA (cytosine-5) methyltransferase (M.HhaI) variant I2L/Q237A, S-adenosyl-L-methionine |
| HhaI DNA (cytosine-5) methyltransferase (M.HhaI)variant I2L/Q237E | — | 37mer DNA duplex (oligonucleotides 11 and 12, single GCGC site) | 0.025 | 2.21 × 10⁻⁴ | 110 | pH 7.4, 37 °C | Open record: HhaI DNA (cytosine-5) methyltransferase (M.HhaI) variant I2L/Q237E, 37mer DNA duplex (oligonucleotides 11 and 12, single GCGC site) |
| HhaI DNA (cytosine-5) methyltransferase (M.HhaI)variant I2L/Q237E | — | S-adenosyl-L-methionine | — | 2.4 × 10⁻⁵ | — | pH 7.4, 37 °C | Open record: HhaI DNA (cytosine-5) methyltransferase (M.HhaI) variant I2L/Q237E, S-adenosyl-L-methionine |
| HhaI DNA (cytosine-5) methyltransferase (M.HhaI)variant I2L/Q237H | — | 37mer DNA duplex (oligonucleotides 11 and 12, single GCGC site) | 0.0033 | 1.41 × 10⁻⁴ | 30 | pH 7.4, 37 °C | Open record: HhaI DNA (cytosine-5) methyltransferase (M.HhaI) variant I2L/Q237H, 37mer DNA duplex (oligonucleotides 11 and 12, single GCGC site) |
| HhaI DNA (cytosine-5) methyltransferase (M.HhaI)variant I2L/Q237H | — | S-adenosyl-L-methionine | — | 1.8 × 10⁻⁵ | — | pH 7.4, 37 °C | Open record: HhaI DNA (cytosine-5) methyltransferase (M.HhaI) variant I2L/Q237H, S-adenosyl-L-methionine |
| HhaI DNA (cytosine-5) methyltransferase (M.HhaI)variant I2L/Q237L | — | 37mer DNA duplex (oligonucleotides 11 and 12, single GCGC site) | 0.0233 | 1.2 × 10⁻⁴ | 190 | pH 7.4, 37 °C | Open record: HhaI DNA (cytosine-5) methyltransferase (M.HhaI) variant I2L/Q237L, 37mer DNA duplex (oligonucleotides 11 and 12, single GCGC site) |
| HhaI DNA (cytosine-5) methyltransferase (M.HhaI)variant I2L/Q237L | — | S-adenosyl-L-methionine | — | 2.4 × 10⁻⁵ | — | pH 7.4, 37 °C | Open record: HhaI DNA (cytosine-5) methyltransferase (M.HhaI) variant I2L/Q237L, S-adenosyl-L-methionine |
| HhaI DNA (cytosine-5) methyltransferase (M.HhaI)variant I2L/Q237S | — | 37mer DNA duplex (oligonucleotides 11 and 12, single GCGC site) | 0.0038 | 1.33 × 10⁻⁴ | 28 | pH 7.4, 37 °C | Open record: HhaI DNA (cytosine-5) methyltransferase (M.HhaI) variant I2L/Q237S, 37mer DNA duplex (oligonucleotides 11 and 12, single GCGC site) |
| HhaI DNA (cytosine-5) methyltransferase (M.HhaI)variant I2L/Q237S | — | S-adenosyl-L-methionine | — | 1.7 × 10⁻⁵ | — | pH 7.4, 37 °C | Open record: HhaI DNA (cytosine-5) methyltransferase (M.HhaI) variant I2L/Q237S, S-adenosyl-L-methionine |