CatLogenzyme kinetics catalog

EC 2.1.1.37: DNA (cytosine-5-)-methyltransferase

TransferasesOpen in Browse

CatLog read 36 records for EC 2.1.1.37 from 3 papers, for 2 organisms. Names in these papers: DNA (cytosine-5) methyltransferase 1 (DNMT1), HhaI DNA (cytosine-5) methyltransferase (M.HhaI), DNMT3A.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 24 records for EC 2.1.1.37 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.1.1.37 in Browse.

Papers

PaperOrganismsRecords
Recombinant human DNA (cytosine-5) methyltransferase. III. Allosteric control, reaction order, and influence of plasmid topology and triplet repeat length on methylation of the fragile X CGG.CCG…Homo sapiens14
Functional analysis of Gln-237 mutants of HhaI methyltransferase—12
Live-cell DNMT3A "catalysome" mapping using engineered methyl-transfer pathwaysMus musculus10

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
DNA (cytosine-5) methyltransferase 1 (DNMT1)Homo sapiens(CGG·CCG)735.6 × 10⁻⁴1 × 10⁻⁴5.6PMID 11359795Open
DNA (cytosine-5) methyltransferase 1 (DNMT1)Homo sapienspRW3602 linear0.00170.020.083PMID 11359795Open
DNA (cytosine-5) methyltransferase 1 (DNMT1)Homo sapienspRW3602 relaxed circular5.6 × 10⁻⁴0.0030.19PMID 11359795Open
DNA (cytosine-5) methyltransferase 1 (DNMT1)Homo sapienspRW3602 supercoiled (−<σ> = 0.045)0.00280.0230.11PMID 11359795Open
DNA (cytosine-5) methyltransferase 1 (DNMT1)Homo sapiensS-adenosyl-L-methioninemean of two determinations—0.011—PMID 11359795Open
DNA (cytosine-5) methyltransferase 1 (DNMT1)Homo sapiensS-adenosyl-L-methioninemean of two determinations; pRW3602 relaxed circular—0.006—PMID 11359795Open
DNA (cytosine-5) methyltransferase 1 (DNMT1)Homo sapiensS-adenosyl-L-methioninemean of two determinations; pRW3602 linear—0.007—PMID 11359795Open
DNA (cytosine-5) methyltransferase 1 (DNMT1)Homo sapiensS-adenosyl-L-methionine(CGG·CCG)73—0.014—PMID 11359795Open
DNA (cytosine-5) methyltransferase 1 (DNMT1)variant Δ501Homo sapiens(CGG·CCG)120.00318 × 10⁻⁴3.9PMID 11359795Open
DNA (cytosine-5) methyltransferase 1 (DNMT1)variant Δ501Homo sapiens(CGG·Cm5CG)120.02642 × 10⁻⁴132PMID 11359795Open
DNA (cytosine-5) methyltransferase 1 (DNMT1)variant Δ501Homo sapienspoly(dI-dC)·poly(dI-dC)0.14537 × 10⁻⁴207.5PMID 11359795Open
DNA (cytosine-5) methyltransferase 1 (DNMT1)variant Δ501Homo sapiensS-adenosyl-L-methioninecosubstrate poly(dI-dC)·poly(dI-dC)—0.009—PMID 11359795Open
DNA (cytosine-5) methyltransferase 1 (DNMT1)variant Δ501Homo sapiensS-adenosyl-L-methioninecosubstrate (CGG·CCG)12—0.004—PMID 11359795Open
DNA (cytosine-5) methyltransferase 1 (DNMT1)variant Δ501Homo sapiensS-adenosyl-L-methioninecosubstrate (CGG·Cm5CG)12—0.004—PMID 11359795Open
DNMT3AMus musculusS-adenosyl-L-methioninecosubstrate poly(dI-dC)∙poly(dI-dC)3.3 × 10⁻⁴3.6 × 10⁻⁴0.93 calc.pH 7.4, 37 °CPMID 42770327Open
DNMT3AMus musculusS-adenosyl-L-methioninecosubstrate poly(dG-dC)∙poly(dG-dC)8.1 × 10⁻⁵3 × 10⁻⁴0.27 calc.pH 7.4, 37 °CPMID 42770327Open
DNMT3Avariant R887AMus musculusAdo-6-azidecosubstrate poly(dI-dC)∙poly(dI-dC)2.8 × 10⁻⁵4.4 × 10⁻⁴0.063 calc.pH 7.4, 37 °CPMID 42770327Open
DNMT3Avariant R887AMus musculusAdo-6-azidecosubstrate poly(dG-dC)∙poly(dG-dC)2.1 × 10⁻⁶3.2 × 10⁻⁴0.0066 calc.pH 7.4, 37 °CPMID 42770327Open
DNMT3Avariant R887AMus musculusS-adenosyl-L-methioninecosubstrate poly(dI-dC)∙poly(dI-dC)3.3 × 10⁻⁴3 × 10⁻⁴1.1 calc.pH 7.4, 37 °CPMID 42770327Open
DNMT3Avariant R887AMus musculusS-adenosyl-L-methioninecosubstrate poly(dG-dC)∙poly(dG-dC)7.5 × 10⁻⁵3.7 × 10⁻⁴0.20 calc.pH 7.4, 37 °CPMID 42770327Open
DNMT3Avariant R887GMus musculusAdo-6-azidecosubstrate poly(dI-dC)∙poly(dI-dC)6.1 × 10⁻⁵4.4 × 10⁻⁴0.14 calc.pH 7.4, 37 °CPMID 42770327Open
DNMT3Avariant R887GMus musculusAdo-6-azidecosubstrate poly(dG-dC)∙poly(dG-dC)5.17 × 10⁻⁶4.2 × 10⁻⁴0.012 calc.pH 7.4, 37 °CPMID 42770327Open
DNMT3Avariant R887GMus musculusS-adenosyl-L-methioninecosubstrate poly(dI-dC)∙poly(dI-dC)2.28 × 10⁻⁴7.2 × 10⁻⁴0.32 calc.pH 7.4, 37 °CPMID 42770327Open
DNMT3Avariant R887GMus musculusS-adenosyl-L-methioninecosubstrate poly(dG-dC)∙poly(dG-dC)2.8 × 10⁻⁵8.5 × 10⁻⁴0.033 calc.pH 7.4, 37 °CPMID 42770327Open
HhaI DNA (cytosine-5) methyltransferase (M.HhaI)—37mer DNA duplex (oligonucleotides 11 and 12, single GCGC site)0.05336.9 × 10⁻⁵770pH 7.4, 37 °CPMID 7899082Open
HhaI DNA (cytosine-5) methyltransferase (M.HhaI)—S-adenosyl-L-methionine—1.5 × 10⁻⁵—pH 7.4, 37 °CPMID 7899082Open
HhaI DNA (cytosine-5) methyltransferase (M.HhaI)variant I2L/Q237A—37mer DNA duplex (oligonucleotides 11 and 12, single GCGC site)0.00588.1 × 10⁻⁵70pH 7.4, 37 °CPMID 7899082Open
HhaI DNA (cytosine-5) methyltransferase (M.HhaI)variant I2L/Q237A—S-adenosyl-L-methionine—1.7 × 10⁻⁵—pH 7.4, 37 °CPMID 7899082Open
HhaI DNA (cytosine-5) methyltransferase (M.HhaI)variant I2L/Q237E—37mer DNA duplex (oligonucleotides 11 and 12, single GCGC site)0.0252.21 × 10⁻⁴110pH 7.4, 37 °CPMID 7899082Open
HhaI DNA (cytosine-5) methyltransferase (M.HhaI)variant I2L/Q237E—S-adenosyl-L-methionine—2.4 × 10⁻⁵—pH 7.4, 37 °CPMID 7899082Open
HhaI DNA (cytosine-5) methyltransferase (M.HhaI)variant I2L/Q237H—37mer DNA duplex (oligonucleotides 11 and 12, single GCGC site)0.00331.41 × 10⁻⁴30pH 7.4, 37 °CPMID 7899082Open
HhaI DNA (cytosine-5) methyltransferase (M.HhaI)variant I2L/Q237H—S-adenosyl-L-methionine—1.8 × 10⁻⁵—pH 7.4, 37 °CPMID 7899082Open
HhaI DNA (cytosine-5) methyltransferase (M.HhaI)variant I2L/Q237L—37mer DNA duplex (oligonucleotides 11 and 12, single GCGC site)0.02331.2 × 10⁻⁴190pH 7.4, 37 °CPMID 7899082Open
HhaI DNA (cytosine-5) methyltransferase (M.HhaI)variant I2L/Q237L—S-adenosyl-L-methionine—2.4 × 10⁻⁵—pH 7.4, 37 °CPMID 7899082Open
HhaI DNA (cytosine-5) methyltransferase (M.HhaI)variant I2L/Q237S—37mer DNA duplex (oligonucleotides 11 and 12, single GCGC site)0.00381.33 × 10⁻⁴28pH 7.4, 37 °CPMID 7899082Open
HhaI DNA (cytosine-5) methyltransferase (M.HhaI)variant I2L/Q237S—S-adenosyl-L-methionine—1.7 × 10⁻⁵—pH 7.4, 37 °CPMID 7899082Open