CatLogenzyme kinetics catalog

Values CatLog read from this paper

Functional roles of Trp337 and Glu632 in Clostridium glucoamylase, as determined by chemical modification, mutagenesis, and the stopped-flow method

PMID 7906268doi:10.1016/s0021-9258(17)41891-6Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 30 records (60 values) from this paper: glucoamylase (CGA) from Clostridium sp. G0005.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 30 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 3.2.1.3

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
glucoamylase (CGA)Clostridium sp. G0005Maltoheptaose60.520.24250 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)Clostridium sp. G0005Maltohexaose53.980.18300 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)Clostridium sp. G0005Maltopentaose59.240.21280 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)Clostridium sp. G0005Maltose6.922.462.81 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)Clostridium sp. G0005Maltotetraose59.470.27220 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)Clostridium sp. G0005Maltotriose45.550.41110 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)variant W321FClostridium sp. G0005Maltoheptaose29.440.2150 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)variant W321FClostridium sp. G0005Maltohexaose24.190.16150 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)variant W321FClostridium sp. G0005Maltopentaose26.540.18150 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)variant W321FClostridium sp. G0005Maltose6.83.12.2 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)variant W321FClostridium sp. G0005Maltotetraose28.560.19150 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)variant W321FClostridium sp. G0005Maltotriose24.810.3375 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)variant W337FClostridium sp. G0005Maltoheptaose0.0440.041.1 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)variant W337FClostridium sp. G0005Maltohexaose0.0410.041.0 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)variant W337FClostridium sp. G0005Maltopentaose0.0520.090.58 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)variant W337FClostridium sp. G0005Maltose0.0060.50.012 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)variant W337FClostridium sp. G0005Maltotetraose0.0520.130.40 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)variant W337FClostridium sp. G0005Maltotriose0.0320.180.18 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)variant W433FClostridium sp. G0005Maltoheptaose49.220.32150 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)variant W433FClostridium sp. G0005Maltohexaose38.490.25150 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)variant W433FClostridium sp. G0005Maltopentaose45.530.24190 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)variant W433FClostridium sp. G0005Maltose4.222.591.63 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)variant W433FClostridium sp. G0005Maltotetraose45.750.33140 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)variant W433FClostridium sp. G0005Maltotriose33.540.4771 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)variant W569FClostridium sp. G0005Maltoheptaose65.380.29230 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)variant W569FClostridium sp. G0005Maltohexaose55.710.22250 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)variant W569FClostridium sp. G0005Maltopentaose60.170.24250 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)variant W569FClostridium sp. G0005Maltose6.672.562.61 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)variant W569FClostridium sp. G0005Maltotetraose61.660.3210 calc.pH 4.5, 25 °COpen
glucoamylase (CGA)variant W569FClostridium sp. G0005Maltotriose46.610.37130 calc.pH 4.5, 25 °COpen