Values CatLog read from this paper
Overexpression and mutagenesis of the cDNA for rat liver 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase. Role of cysteines and tyrosines in catalysis
PMID 8175784doi:10.1016/s0021-9258(17)36860-6Open in Browse
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CatLog read 32 records (96 values) from this paper: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) from Rattus norvegicus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) | Rattus norvegicus | 3α-hydroxy-5α-androstan-17-onerecombinant | 1.26 | 0.0463 | 27 | pH 7, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD), 3α-hydroxy-5α-androstan-17-one |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) | Rattus norvegicus | 3α-hydroxy-5α-androstan-17-onepurified from rat liver (native enzyme) | 2.17 | 0.057 | 38 | pH 7, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD), 3α-hydroxy-5α-androstan-17-one |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) | Rattus norvegicus | 4-Nitroacetophenonerecombinant | 1.09 | 0.23 | 5 | pH 6, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD), 4-Nitroacetophenone |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) | Rattus norvegicus | 4-Nitroacetophenonepurified from rat liver (native enzyme) | 1.31 | 0.134 | 10 | pH 6, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD), 4-Nitroacetophenone |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) | Rattus norvegicus | 4-Nitrobenzaldehyderecombinant | 1.1 | 0.044 | 25 | pH 6, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD), 4-Nitrobenzaldehyde |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) | Rattus norvegicus | 4-Nitrobenzaldehydepurified from rat liver (native enzyme) | 2.02 | 0.0555 | 37 | pH 6, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD), 4-Nitrobenzaldehyde |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) | Rattus norvegicus | 5α-androstan-3,17-dionerecombinant | 0.28 | 0.0041 | 68 | pH 7, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD), 5α-androstan-3,17-dione |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) | Rattus norvegicus | 5α-androstan-3,17-dionepurified from rat liver (native enzyme) | 0.54 | 0.0063 | 85 | pH 7, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD), 5α-androstan-3,17-dione |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) | Rattus norvegicus | 9,10-Phenanthrenequinonerecombinant | 2.22 | 0.0013 | 1.7 × 10³ | pH 6, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD), 9,10-Phenanthrenequinone |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) | Rattus norvegicus | 9,10-Phenanthrenequinonepurified from rat liver (native enzyme) | 3.62 | 0.0019 | 1.9 × 10³ | pH 6, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD), 9,10-Phenanthrenequinone |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) | Rattus norvegicus | NAD+recombinant | 1.14 | 0.94 | 1.2 | pH 7, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD), NAD+ |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) | Rattus norvegicus | NAD+purified from rat liver (native enzyme) | 2.05 | 0.725 | 2.8 | pH 7, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD), NAD+ |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) | Rattus norvegicus | NADHrecombinant | 0.29 | 0.0267 | 12 | pH 7, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD), NADH |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) | Rattus norvegicus | NADHpurified from rat liver (native enzyme) | 0.43 | 0.0267 | 17 | pH 7, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD), NADH |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) | Rattus norvegicus | trans-1,2-dihydroxy-3,5-cyclohexadienerecombinant | 1.18 | 1.4 | 0.83 | pH 9, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD), trans-1,2-dihydroxy-3,5-cyclohexadiene |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) | Rattus norvegicus | trans-1,2-dihydroxy-3,5-cyclohexadienepurified from rat liver (native enzyme) | 1.7 | 1.4 | 1.2 | pH 9, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD), trans-1,2-dihydroxy-3,5-cyclohexadiene |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD)variant C170A | Rattus norvegicus | 3α-hydroxy-5α-androstan-17-one | 2.07 | 0.0429 | 50 | pH 7, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) variant C170A, 3α-hydroxy-5α-androstan-17-one |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD)variant C170A | Rattus norvegicus | 5α-androstan-3,17-dione | 0.45 | 0.0035 | 130 | pH 7, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) variant C170A, 5α-androstan-3,17-dione |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD)variant C170A | Rattus norvegicus | NAD+ | 1.68 | 0.86 | 2 | pH 7, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) variant C170A, NAD+ |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD)variant C170A | Rattus norvegicus | NADH | 0.42 | 0.0231 | 18 | pH 7, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) variant C170A, NADH |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD)variant C217A | Rattus norvegicus | 3α-hydroxy-5α-androstan-17-one | 1.19 | 0.0652 | 18 | pH 7, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) variant C217A, 3α-hydroxy-5α-androstan-17-one |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD)variant C217A | Rattus norvegicus | 5α-androstan-3,17-dione | 0.53 | 0.0187 | 28 | pH 7, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) variant C217A, 5α-androstan-3,17-dione |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD)variant C217A | Rattus norvegicus | NAD+ | 0.85 | 0.71 | 1.3 | pH 7, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) variant C217A, NAD+ |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD)variant C217A | Rattus norvegicus | NADH | 0.43 | 0.0505 | 8.3 | pH 7, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) variant C217A, NADH |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD)variant C242A | Rattus norvegicus | 3α-hydroxy-5α-androstan-17-one | 1.47 | 0.0298 | 50 | pH 7, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) variant C242A, 3α-hydroxy-5α-androstan-17-one |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD)variant C242A | Rattus norvegicus | 5α-androstan-3,17-dione | 0.41 | 0.0058 | 70 | pH 7, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) variant C242A, 5α-androstan-3,17-dione |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD)variant C242A | Rattus norvegicus | NAD+ | 1.59 | 1 | 1.7 | pH 7, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) variant C242A, NAD+ |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD)variant C242A | Rattus norvegicus | NADH | 0.36 | 0.0205 | 18 | pH 7, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) variant C242A, NADH |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD)variant Y205F | Rattus norvegicus | 3α-hydroxy-5α-androstan-17-one | 1.14 | 0.0292 | 40 | pH 7, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) variant Y205F, 3α-hydroxy-5α-androstan-17-one |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD)variant Y205F | Rattus norvegicus | 5α-androstan-3,17-dione | 0.27 | 0.0042 | 65 | pH 7, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) variant Y205F, 5α-androstan-3,17-dione |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD)variant Y205F | Rattus norvegicus | NAD+ | 1.43 | 0.83 | 1.7 | pH 7, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) variant Y205F, NAD+ |
| 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD)variant Y205F | Rattus norvegicus | NADH | 0.36 | 0.0303 | 12 | pH 7, 25 °C | Open record: 3α-hydroxysteroid/dihydrodiol dehydrogenase (3α-HSD) variant Y205F, NADH |