Values CatLog read from this paper

Threonine 246 at the active site of the L-lactate dehydrogenase of Bacillus stearothermophilus is important for catalysis but not for substrate binding

PMID 8251493doi:10.1021/bi00210a023Open in Browse

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CatLog read 35 records (97 values) from this paper: L-lactate dehydrogenase from Bacillus stearothermophilus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.27

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
L-lactate dehydrogenase	Bacillus stearothermophilus	2-oxobutanoic acid	124	0.4	310	pH 6, 25 °C	Open record: L-lactate dehydrogenase, 2-oxobutanoic acid
L-lactate dehydrogenase	Bacillus stearothermophilus	2-oxopentanoic acid	17	2.6	6.5	pH 6, 25 °C	Open record: L-lactate dehydrogenase, 2-oxopentanoic acid
L-lactate dehydrogenase	Bacillus stearothermophilus	3-hydroxy-2-oxopropanoic acid	162	0.43	380	pH 6, 25 °C	Open record: L-lactate dehydrogenase, 3-hydroxy-2-oxopropanoic acid
L-lactate dehydrogenase	Bacillus stearothermophilus	3-methyl-2-oxobutanoic acid	0.26	22	0.012	pH 6, 25 °C	Open record: L-lactate dehydrogenase, 3-methyl-2-oxobutanoic acid
L-lactate dehydrogenase	Bacillus stearothermophilus	3-phenyl-2-oxopropanoic acid	75	0.74	100	pH 6, 25 °C	Open record: L-lactate dehydrogenase, 3-phenyl-2-oxopropanoic acid
L-lactate dehydrogenase	Bacillus stearothermophilus	NADH	—	0.013	—	pH 6, 25 °C	Open record: L-lactate dehydrogenase, NADH
L-lactate dehydrogenase	Bacillus stearothermophilus	pyruvate5 mM FBP	166	0.04	4.2 × 10³	pH 6, 25 °C	Open record: L-lactate dehydrogenase, pyruvate
L-lactate dehydrogenase	Bacillus stearothermophilus	pyruvatewithout FBP (activator omitted)	247	5	49	pH 6, 25 °C	Open record: L-lactate dehydrogenase, pyruvate
L-lactate dehydrogenasevariant T246A	Bacillus stearothermophilus	2-oxobutanoic acid	1.7	0.44	3.9	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246A, 2-oxobutanoic acid
L-lactate dehydrogenasevariant T246A	Bacillus stearothermophilus	2-oxopentanoic acid	1.2	4.2	0.29	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246A, 2-oxopentanoic acid
L-lactate dehydrogenasevariant T246A	Bacillus stearothermophilus	3-hydroxy-2-oxopropanoic acid	20	1	20	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246A, 3-hydroxy-2-oxopropanoic acid
L-lactate dehydrogenasevariant T246A	Bacillus stearothermophilus	3-methyl-2-oxobutanoic acid	0.2	14	0.014	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246A, 3-methyl-2-oxobutanoic acid
L-lactate dehydrogenasevariant T246A	Bacillus stearothermophilus	3-phenyl-2-oxopropanoic acid	4.8	3.1	1.5	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246A, 3-phenyl-2-oxopropanoic acid
L-lactate dehydrogenasevariant T246A	Bacillus stearothermophilus	NADH	—	8 × 10⁻⁴	—	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246A, NADH
L-lactate dehydrogenasevariant T246A	Bacillus stearothermophilus	pyruvate5 mM FBP	5.1	0.045	110	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246A, pyruvate
L-lactate dehydrogenasevariant T246A	Bacillus stearothermophilus	pyruvatewithout FBP (activator omitted)	5.2	0.44	12	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246A, pyruvate
L-lactate dehydrogenasevariant T246L	Bacillus stearothermophilus	2-oxobutanoic acid	1	47	0.021	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246L, 2-oxobutanoic acid
L-lactate dehydrogenasevariant T246L	Bacillus stearothermophilus	2-oxopentanoic acid	0.09	50	0.0018	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246L, 2-oxopentanoic acid
L-lactate dehydrogenasevariant T246L	Bacillus stearothermophilus	3-phenyl-2-oxopropanoic acid	0.18	4.6	0.039	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246L, 3-phenyl-2-oxopropanoic acid
L-lactate dehydrogenasevariant T246L	Bacillus stearothermophilus	pyruvate	10	40	0.25	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246L, pyruvate
L-lactate dehydrogenasevariant T246S	Bacillus stearothermophilus	2-oxobutanoic acid	40	1.1	36	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246S, 2-oxobutanoic acid
L-lactate dehydrogenasevariant T246S	Bacillus stearothermophilus	2-oxopentanoic acid	9.4	3.7	2.5	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246S, 2-oxopentanoic acid
L-lactate dehydrogenasevariant T246S	Bacillus stearothermophilus	3-hydroxy-2-oxopropanoic acid	74	0.54	140	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246S, 3-hydroxy-2-oxopropanoic acid
L-lactate dehydrogenasevariant T246S	Bacillus stearothermophilus	3-methyl-2-oxobutanoic acid	0.23	25	0.0092	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246S, 3-methyl-2-oxobutanoic acid
L-lactate dehydrogenasevariant T246S	Bacillus stearothermophilus	3-phenyl-2-oxopropanoic acid	25	1.8	14	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246S, 3-phenyl-2-oxopropanoic acid
L-lactate dehydrogenasevariant T246S	Bacillus stearothermophilus	NADH	—	0.0066	—	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246S, NADH
L-lactate dehydrogenasevariant T246S	Bacillus stearothermophilus	pyruvate5 mM FBP	132	0.07	1.9 × 10³	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246S, pyruvate
L-lactate dehydrogenasevariant T246S	Bacillus stearothermophilus	pyruvatewithout FBP (activator omitted)	133	10	13	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246S, pyruvate
L-lactate dehydrogenasevariant T246V	Bacillus stearothermophilus	2-oxobutanoic acid	6.9	4.8	1.4	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246V, 2-oxobutanoic acid
L-lactate dehydrogenasevariant T246V	Bacillus stearothermophilus	2-oxopentanoic acid	0.6	6.9	0.087	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246V, 2-oxopentanoic acid
L-lactate dehydrogenasevariant T246V	Bacillus stearothermophilus	3-hydroxy-2-oxopropanoic acid	78	9	8.6	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246V, 3-hydroxy-2-oxopropanoic acid
L-lactate dehydrogenasevariant T246V	Bacillus stearothermophilus	3-phenyl-2-oxopropanoic acid	2.7	2.7	1	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246V, 3-phenyl-2-oxopropanoic acid
L-lactate dehydrogenasevariant T246V	Bacillus stearothermophilus	NADH	—	7 × 10⁻⁴	—	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246V, NADH
L-lactate dehydrogenasevariant T246V	Bacillus stearothermophilus	pyruvate5 mM FBP	10	0.09	110	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246V, pyruvate
L-lactate dehydrogenasevariant T246V	Bacillus stearothermophilus	pyruvatewithout FBP (activator omitted)	11.8	0.97	12	pH 6, 25 °C	Open record: L-lactate dehydrogenase variant T246V, pyruvate