Values CatLog read from this paper
Evidence for lysine 80 as general base catalyst of leucine dehydrogenase
PMID 8262941doi:10.1016/s0021-9258(19)74215-xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 25 records (32 values) from this paper: leucine dehydrogenase from Bacillus stearothermophilus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| leucine dehydrogenase | Bacillus stearothermophilus | 4-methyl-2-oxopentanoate | 280 | 0.88 | 320 calc. | pH 9.7, 25 °C | Open record: leucine dehydrogenase, 4-methyl-2-oxopentanoate |
| leucine dehydrogenase | Bacillus stearothermophilus | ammonia | — | 74.8 | — | 25 °C | Open record: leucine dehydrogenase, ammonia |
| leucine dehydrogenase | Bacillus stearothermophilus | L-leucineoxidative deamination; 10 mM L-leucine/1.25 mM NAD+ standard | 50 | 5.1 | 9.8 calc. | pH 10.5, 25 °C | Open record: leucine dehydrogenase, L-leucine |
| leucine dehydrogenase | Bacillus stearothermophilus | L-leucineL-leucine varied 1.5-10 mM; in H2O | 39.1 | — | — | pH 10.5, 25 °C | Open record: leucine dehydrogenase, L-leucine |
| leucine dehydrogenase | Bacillus stearothermophilus | L-leucine | 29.7 | — | — | 25 °C | Open record: leucine dehydrogenase, L-leucine |
| leucine dehydrogenase | Bacillus stearothermophilus | NAD+ | — | 0.063 | — | pH 10.5, 25 °C | Open record: leucine dehydrogenase, NAD+ |
| leucine dehydrogenase | Bacillus stearothermophilus | NADH | — | 0.035 | — | pH 9.7, 25 °C | Open record: leucine dehydrogenase, NADH |
| leucine dehydrogenasevariant K80A | Bacillus stearothermophilus | 4-methyl-2-oxopentanoate | 250 | 9.8 | 26 calc. | pH 9.7, 25 °C | Open record: leucine dehydrogenase variant K80A, 4-methyl-2-oxopentanoate |
| leucine dehydrogenasevariant K80A | Bacillus stearothermophilus | L-leucineoxidative deamination; 10 mM L-leucine/1.25 mM NAD+ standard | 0.78 | — | — | pH 10.5, 25 °C | Open record: leucine dehydrogenase variant K80A, L-leucine |
| leucine dehydrogenasevariant K80A | Bacillus stearothermophilus | L-leucineoxidative deamination; 10 mM L-leucine/1.25 mM NAD+ standard | — | 3.7 | — | pH 10.5, 25 °C | Open record: leucine dehydrogenase variant K80A, L-leucine |
| leucine dehydrogenasevariant K80A | Bacillus stearothermophilus | L-leucineoxidative deamination; 10 mM L-leucine/1.25 mM NAD+ standard | 9.7 | — | — | pH 10.5, 25 °C | Open record: leucine dehydrogenase variant K80A, L-leucine |
| leucine dehydrogenasevariant K80A | Bacillus stearothermophilus | L-leucineoxidative deamination; 10 mM L-leucine/1.25 mM NAD+ standard | — | 1.2 | — | pH 10.5, 25 °C | Open record: leucine dehydrogenase variant K80A, L-leucine |
| leucine dehydrogenasevariant K80A | Bacillus stearothermophilus | L-leucineL-leucine varied 1.5-10 mM; in H2O; apparent kcat | 1.17 | — | — | pH 10.5, 25 °C | Open record: leucine dehydrogenase variant K80A, L-leucine |
| leucine dehydrogenasevariant K80A | Bacillus stearothermophilus | L-leucine | 0.368 | — | — | 25 °C | Open record: leucine dehydrogenase variant K80A, L-leucine |
| leucine dehydrogenasevariant K80A | Bacillus stearothermophilus | NAD+ | — | 0.14 | — | pH 10.5, 25 °C | Open record: leucine dehydrogenase variant K80A, NAD+ |
| leucine dehydrogenasevariant K80A | Bacillus stearothermophilus | NAD+in the presence of 1 M ammonia (Table I footnote a) | — | 0.041 | — | pH 10.5, 25 °C | Open record: leucine dehydrogenase variant K80A, NAD+ |
| leucine dehydrogenasevariant K80A | Bacillus stearothermophilus | NADH | — | 0.022 | — | pH 9.7, 25 °C | Open record: leucine dehydrogenase variant K80A, NADH |
| leucine dehydrogenasevariant K80Q | Bacillus stearothermophilus | 4-methyl-2-oxopentanoate | 66 | 25 | 2.6 calc. | pH 9.7, 25 °C | Open record: leucine dehydrogenase variant K80Q, 4-methyl-2-oxopentanoate |
| leucine dehydrogenasevariant K80Q | Bacillus stearothermophilus | L-leucine | 0.54 | 17 | 0.032 calc. | pH 10.5, 25 °C | Open record: leucine dehydrogenase variant K80Q, L-leucine |
| leucine dehydrogenasevariant K80Q | Bacillus stearothermophilus | NAD+ | — | 0.034 | — | pH 10.5, 25 °C | Open record: leucine dehydrogenase variant K80Q, NAD+ |
| leucine dehydrogenasevariant K80Q | Bacillus stearothermophilus | NADH | — | 0.045 | — | pH 9.7, 25 °C | Open record: leucine dehydrogenase variant K80Q, NADH |
| leucine dehydrogenasevariant K80R | Bacillus stearothermophilus | 4-methyl-2-oxopentanoate | 1.7 | 0.99 | 1.7 calc. | pH 9.7, 25 °C | Open record: leucine dehydrogenase variant K80R, 4-methyl-2-oxopentanoate |
| leucine dehydrogenasevariant K80R | Bacillus stearothermophilus | L-leucine | 0.11 | 2.8 | 0.039 calc. | pH 10.5, 25 °C | Open record: leucine dehydrogenase variant K80R, L-leucine |
| leucine dehydrogenasevariant K80R | Bacillus stearothermophilus | NAD+ | — | 0.074 | — | pH 10.5, 25 °C | Open record: leucine dehydrogenase variant K80R, NAD+ |
| leucine dehydrogenasevariant K80R | Bacillus stearothermophilus | NADH | — | 0.037 | — | pH 9.7, 25 °C | Open record: leucine dehydrogenase variant K80R, NADH |