CatLogenzyme kinetics catalog

Values CatLog read from this paper

Purification and characterization of a novel dimeric 20 alpha-hydroxysteroid dehydrogenase from Tetrahymena pyriformis

PMID 8280099doi:10.1042/bj2970195Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 18 records (18 values) from this paper: 20alpha-hydroxysteroid dehydrogenase from Tetrahymena pyriformis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.149

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
20alpha-hydroxysteroid dehydrogenaseTetrahymena pyriformis1,2-Cyclohexanedione—0.125—pH 6.5, 25 °COpen
20alpha-hydroxysteroid dehydrogenaseTetrahymena pyriformis17α,20α-Dihydroxypregn-4-en-3-one—0.013—pH 10, 25 °COpen
20alpha-hydroxysteroid dehydrogenaseTetrahymena pyriformis17α,21-Dihydroxy-5β-pregnane-3,11,20-trione—0.02—pH 6.5, 25 °COpen
20alpha-hydroxysteroid dehydrogenaseTetrahymena pyriformis17α,21-Dihydroxy-5β-pregnane-3,20-dione—0.022—pH 6.5, 25 °COpen
20alpha-hydroxysteroid dehydrogenaseTetrahymena pyriformis17α-hydroxypregn-4-ene-3,20-dione—0.0029—pH 6.5, 25 °COpen
20alpha-hydroxysteroid dehydrogenaseTetrahymena pyriformis2,4-Pentanedione—0.21—pH 6.5, 25 °COpen
20alpha-hydroxysteroid dehydrogenaseTetrahymena pyriformis20α-Hydroxy-5β-pregnan-3-one—0.073—pH 10, 25 °COpen
20alpha-hydroxysteroid dehydrogenaseTetrahymena pyriformis3β,17α-dihydroxypregn-5-en-20-one—0.0034—pH 6.5, 25 °COpen
20alpha-hydroxysteroid dehydrogenaseTetrahymena pyriformis4-Nitrobenzaldehyde—0.34—pH 6.5, 25 °COpen
20alpha-hydroxysteroid dehydrogenaseTetrahymena pyriformisCamphorquinone—0.078—pH 6.5, 25 °COpen
20alpha-hydroxysteroid dehydrogenaseTetrahymena pyriformisD-Glyceraldehyde—0.67—pH 6.5, 25 °COpen
20alpha-hydroxysteroid dehydrogenaseTetrahymena pyriformisEthyl acetoacetate—0.067—pH 6.5, 25 °COpen
20alpha-hydroxysteroid dehydrogenaseTetrahymena pyriformisIsatin—0.064—pH 6.5, 25 °COpen
20alpha-hydroxysteroid dehydrogenaseTetrahymena pyriformisNADP+—0.008—pH 7, 25 °COpen
20alpha-hydroxysteroid dehydrogenaseTetrahymena pyriformisNADPH—2 × 10⁻⁴—pH 7, 25 °COpen
20alpha-hydroxysteroid dehydrogenaseTetrahymena pyriformisPhenylglyoxal—0.43—pH 6.5, 25 °COpen
20alpha-hydroxysteroid dehydrogenaseTetrahymena pyriformisPyridine-3-aldehyde—0.081—pH 6.5, 25 °COpen
20alpha-hydroxysteroid dehydrogenaseTetrahymena pyriformistrans-1,2-dihydroxycyclohexa-3,5-diene—2.1—pH 10, 25 °COpen