CatLogenzyme kinetics catalog

Values CatLog read from this paper

Kinetic mechanism of Escherichia coli isocitrate dehydrogenase

PMID 8369299doi:10.1021/bi00087a007Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 21 records (31 values) from this paper: NADP-dependent isocitrate dehydrogenase from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 10 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.1.1.42

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
NADP-dependent isocitrate dehydrogenaseEscherichia coli(2R)-malate0.00822.10.0039 calc.pH 7.3, 21 °COpen
NADP-dependent isocitrate dehydrogenaseEscherichia coli(2R,3S)-isocitratecosubstrate NADP80.50.0117.3 × 10³ calc.pH 7.3, 21 °COpen
NADP-dependent isocitrate dehydrogenaseEscherichia coli(2R,3S)-isocitratecosubstrate NAD3.220.006540 calc.pH 7.3, 21 °COpen
NADP-dependent isocitrate dehydrogenaseEscherichia coli2-oxoglutarate14.90.5726 calc.pH 7.3, 21 °COpen
NADP-dependent isocitrate dehydrogenaseEscherichia colicarbon dioxide—3.13—pH 7.3, 21 °COpen
NADP-dependent isocitrate dehydrogenaseEscherichia coliNAD+—4.7—pH 7.3, 21 °COpen
NADP-dependent isocitrate dehydrogenaseEscherichia coliNADP+cosubstrate (2R,3S)-isocitrate—0.017—pH 7.3, 21 °COpen
NADP-dependent isocitrate dehydrogenaseEscherichia coliNADP+cosubstrate (2R)-malate—0.195—pH 7.3, 21 °COpen
NADP-dependent isocitrate dehydrogenaseEscherichia coliNADPH—0.007—pH 7.3, 21 °COpen
NADP-dependent isocitrate dehydrogenasevariant S113LEscherichia coli(2R)-malate4.7 × 10⁻⁴5.29.0 × 10⁻⁵ calc.pH 7.3, 21 °COpen
NADP-dependent isocitrate dehydrogenasevariant S113LEscherichia coli(2R,3S)-isocitratecosubstrate NADP0.360.02514 calc.pH 7.3, 21 °COpen
NADP-dependent isocitrate dehydrogenasevariant S113LEscherichia coli(2R,3S)-isocitratecosubstrate NAD6.7 × 10⁻⁴0.1030.0065 calc.pH 7.3, 21 °COpen
NADP-dependent isocitrate dehydrogenasevariant S113LEscherichia coliNAD+—4.3—pH 7.3, 21 °COpen
NADP-dependent isocitrate dehydrogenasevariant S113LEscherichia coliNADP+cosubstrate (2R,3S)-isocitrate—0.176—pH 7.3, 21 °COpen
NADP-dependent isocitrate dehydrogenasevariant S113LEscherichia coliNADP+cosubstrate (2R)-malate—0.42—pH 7.3, 21 °COpen
NADP-dependent isocitrate dehydrogenasevariant S113NEscherichia coli(2R)-malate6.7 × 10⁻⁴16.54.1 × 10⁻⁵ calc.pH 7.3, 21 °COpen
NADP-dependent isocitrate dehydrogenasevariant S113NEscherichia coli(2R,3S)-isocitratecosubstrate NADP3.850.018210 calc.pH 7.3, 21 °COpen
NADP-dependent isocitrate dehydrogenasevariant S113NEscherichia coli(2R,3S)-isocitratecosubstrate NAD0.00920.1190.077 calc.pH 7.3, 21 °COpen
NADP-dependent isocitrate dehydrogenasevariant S113NEscherichia coliNAD+—15.3—pH 7.3, 21 °COpen
NADP-dependent isocitrate dehydrogenasevariant S113NEscherichia coliNADP+cosubstrate (2R,3S)-isocitrate—0.291—pH 7.3, 21 °COpen
NADP-dependent isocitrate dehydrogenasevariant S113NEscherichia coliNADP+cosubstrate (2R)-malate—0.234—pH 7.3, 21 °COpen