Values CatLog read from this paper
Use of endoproteases to identify catalytic domains, linker regions, and functional interactions in soluble peptidylglycine alpha-amidating monooxygenase
PMID 8486658doi:10.1016/s0021-9258(18)98406-1Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 9 records (16 values) from this paper: peptidylglycine alpha-hydroxylating monooxygenase (PHM) activity of PAM, peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) activity of PAM from Rattus norvegicus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) activity of PAM | Rattus norvegicus | N-acetyl-L-tyrosyl-L-valyl-alpha-hydroxyglycinesecreted by stably transfected hEK-293 cells; purified | 92 | 0.037 | 2.5 × 10³ calc. | pH 5.5, 37 °C | Open record: peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) activity of PAM, N-acetyl-L-tyrosyl-L-valyl-alpha-hydroxyglycine |
| peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) activity of PAM | Rattus norvegicus | N-acetyl-L-tyrosyl-L-valyl-alpha-hydroxyglycine | 86 | 0.034 | 2.5 × 10³ calc. | pH 5.5, 37 °C | Open record: peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) activity of PAM, N-acetyl-L-tyrosyl-L-valyl-alpha-hydroxyglycine |
| peptidylglycine alpha-hydroxylating monooxygenase (PHM) activity of PAM | Rattus norvegicus | N-acetyl-L-tyrosyl-L-valyl-glycinesecreted by stably transfected hEK-293 cells; purified | 7 | 0.014 | 500 calc. | pH 5.5, 37 °C | Open record: peptidylglycine alpha-hydroxylating monooxygenase (PHM) activity of PAM, N-acetyl-L-tyrosyl-L-valyl-glycine |
| peptidylglycine alpha-hydroxylating monooxygenase (PHM) activity of PAM | Rattus norvegicus | N-acetyl-L-tyrosyl-L-valyl-glycineTable II | 16 | 0.008 | 2.0 × 10³ calc. | pH 5.5, 37 °C | Open record: peptidylglycine alpha-hydroxylating monooxygenase (PHM) activity of PAM, N-acetyl-L-tyrosyl-L-valyl-glycine |
| peptidylglycine alpha-hydroxylating monooxygenase (PHM) activity of PAM | Rattus norvegicus | N-acetyl-L-tyrosyl-L-valyl-glycineTable II | 14 | 0.009 | 1.6 × 10³ calc. | pH 5.5, 37 °C | Open record: peptidylglycine alpha-hydroxylating monooxygenase (PHM) activity of PAM, N-acetyl-L-tyrosyl-L-valyl-glycine |
| peptidylglycine alpha-hydroxylating monooxygenase (PHM) activity of PAM | Rattus norvegicus | N-acetyl-L-tyrosyl-L-valyl-glycinerPAM-3 digested with endoprotease Lys-C (36-kDa PHM protein) | 17 | 0.011 | 1.5 × 10³ calc. | pH 5.5, 37 °C | Open record: peptidylglycine alpha-hydroxylating monooxygenase (PHM) activity of PAM, N-acetyl-L-tyrosyl-L-valyl-glycine |
| peptidylglycine alpha-hydroxylating monooxygenase (PHM) activity of PAM | Rattus norvegicus | N-acetyl-L-tyrosyl-L-valyl-glycinerPHM-s pool C; secreted by hEK-293 cells; Table II | 4 | 0.018 | 220 calc. | pH 5.5, 37 °C | Open record: peptidylglycine alpha-hydroxylating monooxygenase (PHM) activity of PAM, N-acetyl-L-tyrosyl-L-valyl-glycine |
| peptidylglycine alpha-hydroxylating monooxygenase (PHM) activity of PAM | Rattus norvegicus | N-acetyl-L-tyrosyl-L-valyl-glycinesecreted by stably transfected hEK-293 cells; purified | — | 0.018 | — | pH 5.5, 37 °C | Open record: peptidylglycine alpha-hydroxylating monooxygenase (PHM) activity of PAM, N-acetyl-L-tyrosyl-L-valyl-glycine |
| peptidylglycine alpha-hydroxylating monooxygenase (PHM) activity of PAM | Rattus norvegicus | N-acetyl-L-tyrosyl-L-valyl-glycinerPAM-3 digested with trypsin (0.1 µg trypsin/ug PAM-3) | — | 0.008 | — | pH 5.5, 37 °C | Open record: peptidylglycine alpha-hydroxylating monooxygenase (PHM) activity of PAM, N-acetyl-L-tyrosyl-L-valyl-glycine |