Values CatLog read from this paper
A highly active decarboxylating dehydrogenase with rationally inverted coenzyme specificity
PMID 8524825doi:10.1073/pnas.92.25.11666Open in Browse
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CatLog read 16 records (42 values) from this paper: isocitrate dehydrogenase (IDH) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| isocitrate dehydrogenase (IDH)variant K344D/Y345I | Escherichia coli | NAD | 2.59 | 3.3 | 0.78 | pH 7.5, 21 °C | Open record: isocitrate dehydrogenase (IDH) variant K344D/Y345I, NAD |
| isocitrate dehydrogenase (IDH)variant K344D/Y345I | Escherichia coli | NADP | 6.3 | 7.3 | 0.86 | pH 7.5, 21 °C | Open record: isocitrate dehydrogenase (IDH) variant K344D/Y345I, NADP |
| isocitrate dehydrogenase (IDH)variant K344D/Y345I/V351A | Escherichia coli | NAD | 9.39 | 0.85 | 11 | pH 7.5, 21 °C | Open record: isocitrate dehydrogenase (IDH) variant K344D/Y345I/V351A, NAD |
| isocitrate dehydrogenase (IDH)variant K344D/Y345I/V351A | Escherichia coli | NADP | 18 | 6.4 | 2.8 | pH 7.5, 21 °C | Open record: isocitrate dehydrogenase (IDH) variant K344D/Y345I/V351A, NADP |
| isocitrate dehydrogenase (IDH)variant K344D/Y345I/V351A/Y391G/R395G | Escherichia coli | NAD | 0.284 | 1.4 | 0.2 | pH 7.5, 21 °C | Open record: isocitrate dehydrogenase (IDH) variant K344D/Y345I/V351A/Y391G/R395G, NAD |
| isocitrate dehydrogenase (IDH)variant K344D/Y345I/V351A/Y391K/R395S | Escherichia coli | NAD | 6 | 0.29 | 21 | pH 7.5, 21 °C | Open record: isocitrate dehydrogenase (IDH) variant K344D/Y345I/V351A/Y391K/R395S, NAD |
| isocitrate dehydrogenase (IDH)variant K344D/Y345I/V351A/Y391K/R395S | Escherichia coli | NADP | 2 | 11.3 | 0.18 | pH 7.5, 21 °C | Open record: isocitrate dehydrogenase (IDH) variant K344D/Y345I/V351A/Y391K/R395S, NADP |
| isocitrate dehydrogenase (IDH)variant K344D/Y345I/V351A/Y391K/R395S/C332Y | Escherichia coli | NAD | 11.4 | 0.108 | 106 | pH 7.5, 21 °C | Open record: isocitrate dehydrogenase (IDH) variant K344D/Y345I/V351A/Y391K/R395S/C332Y, NAD |
| isocitrate dehydrogenase (IDH)variant K344D/Y345I/V351A/Y391K/R395S/C332Y | Escherichia coli | NADP | 3.34 | 2.8 | 1.2 | pH 7.5, 21 °C | Open record: isocitrate dehydrogenase (IDH) variant K344D/Y345I/V351A/Y391K/R395S/C332Y, NADP |
| isocitrate dehydrogenase (IDH)variant K344D/Y345I/V351A/Y391K/R395S/C332Y/C201I | Escherichia coli | isocitrate | — | 0.01 | — | pH 7.5, 21 °C | Open record: isocitrate dehydrogenase (IDH) variant K344D/Y345I/V351A/Y391K/R395S/C332Y/C201I, isocitrate |
| isocitrate dehydrogenase (IDH)variant K344D/Y345I/V351A/Y391K/R395S/C332Y/C201I | Escherichia coli | NAD | 16.2 | 0.099 | 164 | pH 7.5, 21 °C | Open record: isocitrate dehydrogenase (IDH) variant K344D/Y345I/V351A/Y391K/R395S/C332Y/C201I, NAD |
| isocitrate dehydrogenase (IDH)variant K344D/Y345I/V351A/Y391K/R395S/C332Y/C201I | Escherichia coli | NADP | 4.7 | 5.8 | 0.81 | pH 7.5, 21 °C | Open record: isocitrate dehydrogenase (IDH) variant K344D/Y345I/V351A/Y391K/R395S/C332Y/C201I, NADP |
| isocitrate dehydrogenase (IDH)variant K344D/Y345I/V351A/Y391K/R395S/Q316L/V317P/G319S/I320L | Escherichia coli | NAD | — | — | 0.96 | pH 7.5, 21 °C | Open record: isocitrate dehydrogenase (IDH) variant K344D/Y345I/V351A/Y391K/R395S/Q316L/V317P/G319S/I320L, NAD |
| isocitrate dehydrogenase (IDH)variant K344D/Y345I/V351A/Y391K/R395S/Q316L/V317P/G319S/I320L | Escherichia coli | NADP | — | — | 0.01 | pH 7.5, 21 °C | Open record: isocitrate dehydrogenase (IDH) variant K344D/Y345I/V351A/Y391K/R395S/Q316L/V317P/G319S/I320L, NADP |
| isocitrate dehydrogenase (IDH)variant K344D/Y345I/V351A/Y391K/R395S/R292D | Escherichia coli | NAD | 9.74 | 0.924 | 11 | pH 7.5, 21 °C | Open record: isocitrate dehydrogenase (IDH) variant K344D/Y345I/V351A/Y391K/R395S/R292D, NAD |
| isocitrate dehydrogenase (IDH)variant K344D/Y345I/V351A/Y391K/R395S/R292D | Escherichia coli | NADP | 0.39 | 32.2 | 0.012 | pH 7.5, 21 °C | Open record: isocitrate dehydrogenase (IDH) variant K344D/Y345I/V351A/Y391K/R395S/R292D, NADP |