CatLogenzyme kinetics catalog

Values CatLog read from this paper

The role of glutamate 87 in the kinetic mechanism of Thermus thermophilus isopropylmalate dehydrogenase

PMID 8535253doi:10.1002/pro.5560041022Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 26 records (38 values) from this paper: isopropylmalate dehydrogenase (IMDH) from Thermus thermophilus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 12 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.1.1.85

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
isopropylmalate dehydrogenase (IMDH)Thermus thermophilus(2R)-2-hydroxybutanedioatecosubstrate NAD10.591.517.01 calc.pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)Thermus thermophilus(2R)-2-hydroxybutanedioatecosubstrate NADP4.6931.570.149 calc.pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)Thermus thermophilus(2R,3S)-2,3-dihydroxybutanedioate (meso-tartrate)cosubstrate NAD0.190.04074.7 calc.pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)Thermus thermophilus(2R,3S)-2,3-dihydroxybutanedioate (meso-tartrate)cosubstrate NADP0.10.8170.12 calc.pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)Thermus thermophilus(2R,3S)-2-hydroxy-3-(propan-2-yl)butanedioatecosubstrate NAD; Table 10.241.7 × 10⁻⁵1.4 × 10⁴ calc.pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)Thermus thermophilus(2R,3S)-2-hydroxy-3-(propan-2-yl)butanedioatecosubstrate NADP; Table 11.660.00298557 calc.pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)Thermus thermophilus(2R,3S)-2-hydroxy-3-(propan-2-yl)butanedioatecosubstrate NAD; in the text——1.4 × 10⁴pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)Thermus thermophilusNAD+cosubstrate 2R,3S-isopropylmalate—0.00321—pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)Thermus thermophilusNAD+cosubstrate 2R,3S-tartrate—0.0454—pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)Thermus thermophilusNAD+cosubstrate 2R-malate—0.1065—pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)Thermus thermophilusNADP+cosubstrate 2R,3S-isopropylmalate—1.836—pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)Thermus thermophilusNADP+cosubstrate 2R,3S-tartrate—1.573—pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)Thermus thermophilusNADP+cosubstrate 2R-malate—6.579—pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)variant E87GThermus thermophilus(2R)-2-hydroxybutanedioate0.2635.590.0073 calc.pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)variant E87GThermus thermophilus(2R,3S)-2,3-dihydroxybutanedioate (meso-tartrate)0.211.210.17 calc.pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)variant E87GThermus thermophilus(2R,3S)-2-hydroxy-3-(propan-2-yl)butanedioate0.24.8 × 10⁻⁴420 calc.pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)variant E87GThermus thermophilusNAD+cosubstrate 2R,3S-isopropylmalate—0.131—pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)variant E87GThermus thermophilusNAD+cosubstrate 2R,3S-tartrate—0.386—pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)variant E87GThermus thermophilusNAD+cosubstrate 2R-malate—2.584—pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)variant E87QThermus thermophilus(2R)-2-hydroxybutanedioateTable 10.1932.60.0058 calc.pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)variant E87QThermus thermophilus(2R)-2-hydroxybutanedioatein the text——0.006pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)variant E87QThermus thermophilus(2R,3S)-2,3-dihydroxybutanedioate (meso-tartrate)0.260.7610.34 calc.pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)variant E87QThermus thermophilus(2R,3S)-2-hydroxy-3-(propan-2-yl)butanedioate0.210.005340 calc.pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)variant E87QThermus thermophilusNAD+cosubstrate 2R,3S-isopropylmalate—0.778—pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)variant E87QThermus thermophilusNAD+cosubstrate 2R,3S-tartrate—0.181—pH 7.3, 21 °COpen
isopropylmalate dehydrogenase (IMDH)variant E87QThermus thermophilusNAD+cosubstrate 2R-malate—1.853—pH 7.3, 21 °COpen