Values CatLog read from this paper
Kinetic and crystallographic studies of Escherichia coli UDP-N-acetylmuramate:L-alanine ligase
PMID 8976565doi:10.1002/pro.5560051219Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 24 records (65 values) from this paper: UDP-N-acetylmuramate:L-alanine ligase (MurC) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| UDP-N-acetylmuramate:L-alanine ligase (MurC) | Escherichia coli | (2S)-2-amino-N-(hydroxymethyl)propanamide | 11.7 | 16 | 0.72 | pH 8, 37 °C | Open record: UDP-N-acetylmuramate:L-alanine ligase (MurC), (2S)-2-amino-N-(hydroxymethyl)propanamide |
| UDP-N-acetylmuramate:L-alanine ligase (MurC) | Escherichia coli | 1-(2-aminopropanoyl)piperidine | 6.2 | 62 | 0.092 | pH 8, 37 °C | Open record: UDP-N-acetylmuramate:L-alanine ligase (MurC), 1-(2-aminopropanoyl)piperidine |
| UDP-N-acetylmuramate:L-alanine ligase (MurC) | Escherichia coli | 2-aminobutanoic acid | 25 | 4.3 | 5.3 | pH 8, 37 °C | Open record: UDP-N-acetylmuramate:L-alanine ligase (MurC), 2-aminobutanoic acid |
| UDP-N-acetylmuramate:L-alanine ligase (MurC) | Escherichia coli | adenosine 5'-triphosphatebiosynthetic ligase reaction (UNAM + ATP + L-alanine) | — | 0.13 | — | pH 8, 37 °C | Open record: UDP-N-acetylmuramate:L-alanine ligase (MurC), adenosine 5'-triphosphate |
| UDP-N-acetylmuramate:L-alanine ligase (MurC) | Escherichia coli | adenosine 5'-triphosphatecontinuous assay (ADP formation); cosubstrate UNAM | 0.53 | 0.022 | 24 calc. | pH 8, 37 °C | Open record: UDP-N-acetylmuramate:L-alanine ligase (MurC), adenosine 5'-triphosphate |
| UDP-N-acetylmuramate:L-alanine ligase (MurC) | Escherichia coli | DL-Propargylglycine | 20 | 1.1 | 18 | pH 8, 37 °C | Open record: UDP-N-acetylmuramate:L-alanine ligase (MurC), DL-Propargylglycine |
| UDP-N-acetylmuramate:L-alanine ligase (MurC) | Escherichia coli | Glycine | 22 | 10 | 2.3 | pH 8, 37 °C | Open record: UDP-N-acetylmuramate:L-alanine ligase (MurC), Glycine |
| UDP-N-acetylmuramate:L-alanine ligase (MurC) | Escherichia coli | L-alaninamide | 18 | 7.9 | 2.3 | pH 8, 37 °C | Open record: UDP-N-acetylmuramate:L-alanine ligase (MurC), L-alaninamide |
| UDP-N-acetylmuramate:L-alanine ligase (MurC) | Escherichia coli | L-Alanine | 16.3 | 0.048 | 330 | pH 8, 37 °C | Open record: UDP-N-acetylmuramate:L-alanine ligase (MurC), L-Alanine |
| UDP-N-acetylmuramate:L-alanine ligase (MurC) | Escherichia coli | L-alanine benzyl ester | 13.5 | 0.36 | 27 | pH 8, 37 °C | Open record: UDP-N-acetylmuramate:L-alanine ligase (MurC), L-alanine benzyl ester |
| UDP-N-acetylmuramate:L-alanine ligase (MurC) | Escherichia coli | L-alanine ethyl ester | 15.2 | 0.59 | 37 | pH 8, 37 °C | Open record: UDP-N-acetylmuramate:L-alanine ligase (MurC), L-alanine ethyl ester |
| UDP-N-acetylmuramate:L-alanine ligase (MurC) | Escherichia coli | L-alanine methyl ester | 28 | 0.5 | 55 | pH 8, 37 °C | Open record: UDP-N-acetylmuramate:L-alanine ligase (MurC), L-alanine methyl ester |
| UDP-N-acetylmuramate:L-alanine ligase (MurC) | Escherichia coli | L-alanine tert-butyl ester | 22 | 0.2 | 100 | pH 8, 37 °C | Open record: UDP-N-acetylmuramate:L-alanine ligase (MurC), L-alanine tert-butyl ester |
| UDP-N-acetylmuramate:L-alanine ligase (MurC) | Escherichia coli | L-Cysteine | 20 | 3.8 | 5 | pH 8, 37 °C | Open record: UDP-N-acetylmuramate:L-alanine ligase (MurC), L-Cysteine |
| UDP-N-acetylmuramate:L-alanine ligase (MurC) | Escherichia coli | L-Isoleucine | 5.2 | 40 | 0.13 | pH 8, 37 °C | Open record: UDP-N-acetylmuramate:L-alanine ligase (MurC), L-Isoleucine |
| UDP-N-acetylmuramate:L-alanine ligase (MurC) | Escherichia coli | L-Serine | 25 | 1.2 | 20 | pH 8, 37 °C | Open record: UDP-N-acetylmuramate:L-alanine ligase (MurC), L-Serine |
| UDP-N-acetylmuramate:L-alanine ligase (MurC) | Escherichia coli | L-Threonine | 23 | 25 | 0.1 | pH 8, 37 °C | Open record: UDP-N-acetylmuramate:L-alanine ligase (MurC), L-Threonine |
| UDP-N-acetylmuramate:L-alanine ligase (MurC) | Escherichia coli | L-Valine | 22 | 70 | 0.28 | pH 8, 37 °C | Open record: UDP-N-acetylmuramate:L-alanine ligase (MurC), L-Valine |
| UDP-N-acetylmuramate:L-alanine ligase (MurC) | Escherichia coli | L-Vinylglycine | 15.3 | 0.44 | 35 | pH 8, 37 °C | Open record: UDP-N-acetylmuramate:L-alanine ligase (MurC), L-Vinylglycine |
| UDP-N-acetylmuramate:L-alanine ligase (MurC) | Escherichia coli | uridine diphosphate-N-acetylmuramatebiosynthetic ligase reaction (UNAM + ATP + L-alanine) | — | 0.044 | — | pH 8, 37 °C | Open record: UDP-N-acetylmuramate:L-alanine ligase (MurC), uridine diphosphate-N-acetylmuramate |
| UDP-N-acetylmuramate:L-alanine ligase (MurC) | Escherichia coli | uridine diphosphate-N-acetylmuramatecontinuous assay (ADP formation); cosubstrate ATP | — | 0.22 | — | pH 8, 37 °C | Open record: UDP-N-acetylmuramate:L-alanine ligase (MurC), uridine diphosphate-N-acetylmuramate |
| UDP-N-acetylmuramate:L-alanine ligase (MurC) | Escherichia coli | β-alanine | 2.5 | 0.046 | 0.92 | pH 8, 37 °C | Open record: UDP-N-acetylmuramate:L-alanine ligase (MurC), β-alanine |
| UDP-N-acetylmuramate:L-alanine ligase (MurC) | Escherichia coli | β-chloro-L-alanine | 20 | 0.14 | 150 | pH 8, 37 °C | Open record: UDP-N-acetylmuramate:L-alanine ligase (MurC), β-chloro-L-alanine |
| UDP-N-acetylmuramate:L-alanine ligase (MurC) | Escherichia coli | β-cyano-L-alanine | 27 | 0.19 | 133 | pH 8, 37 °C | Open record: UDP-N-acetylmuramate:L-alanine ligase (MurC), β-cyano-L-alanine |